Information on EC 2.7.1.47 - D-ribulokinase

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The expected taxonomic range for this enzyme is: Klebsiella aerogenes

EC NUMBER
COMMENTARY hide
2.7.1.47
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RECOMMENDED NAME
GeneOntology No.
D-ribulokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-ribulose = ADP + D-ribulose 5-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-arabinose degradation II
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ribitol degradation
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degradation of pentoses
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Pentose and glucuronate interconversions
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-ribulose 5-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-40-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-arabitol
?
show the reaction diagram
-
poor substrate, not L-isomer
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-
?
ATP + D-ribulose
?
show the reaction diagram
-
inducible enzyme of ribitol catabolism
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-
?
ATP + D-ribulose
ADP + D-ribulose 5-phosphate
show the reaction diagram
ATP + ribitol
?
show the reaction diagram
-
poor substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-ribulose
?
show the reaction diagram
-
inducible enzyme of ribitol catabolism
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation, can replace Mg2+ with 23% efficiency
Mn2+
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activation, can replace Mg2+ with 54% efficiency
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
product inhibition
Mg2+
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inhibition at a Mg/ATP ratio above 2, 11% inhibition at a ratio of 3
additional information
-
no inhibition by D-ribulose 5-phosphate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.83
ATP
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pH 7.7, 27C
140
D-arabitol
-
pH 7.8, 28C
0.4 - 0.92
D-ribulose
220
ribitol
-
pH 7.8, 28C
additional information
additional information
-
kinetic study at 28C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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broad
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10.5
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pH 6.0: about 70% of maximal activity, pH 10.5: about half-maximal activity at pH 10.5
PDB
SCOP
CATH
ORGANISM
UNIPROT
Synechococcus elongatus (strain PCC 7942)
Synechococcus elongatus (strain PCC 7942)
Synechococcus elongatus (strain PCC 7942)
Synechococcus elongatus (strain PCC 7942)
Synechococcus elongatus (strain PCC 7942)
Synechococcus elongatus (strain PCC 7942)
Synechococcus elongatus (strain PCC 7942)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
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2 * 59000, SDS-PAGE
60000
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2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE
112000
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recombinant enzyme expressed in E. coli strain NC629, gel filtration
116000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
D-ribulose stabilizes
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EDTA or 2-mercaptoethanol stabilizes during purification
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in 50% v/v glycerol, in the dark, 1 month
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4C, about 10% loss of activity within 6 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli K12 strain NC629 harbouring multi-copy lambdaprbt-101 prophage which carries the rbt-operon
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