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Information on EC 2.7.1.24 - dephospho-CoA kinase and Organism(s) Escherichia coli and UniProt Accession P0A6I9

for references in articles please use BRENDA:EC2.7.1.24
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This record set is specific for:
Escherichia coli
UNIPROT: P0A6I9 not found.
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
coenzyme a synthase, dpck, dephospho-coa kinase, dephosphocoenzyme a, dephosphocoenzyme a kinase, dephospho-coenzyme a kinase, tk1697, gtp-dependent dpck, ehdpck1, ehdpck2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'-dephospho-CoA kinase
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dephosphocoenzyme A kinase
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dephosphocoenzyme A kinase (phosphorylating)
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kinase, dephosphocoenzyme A (phosphorylating)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 3'-dephospho-CoA = ADP + CoA
show the reaction diagram
active site, mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:3'-dephospho-CoA 3'-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-83-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + dephospho-CoA
ADP + CoA
show the reaction diagram
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-
ir
ATP + dephospho-CoA
ADP + CoA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + dephospho-CoA
ADP + CoA
show the reaction diagram
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final step of coenzyme A biosynthesis
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene caoE, formerly yacE
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22600
1 * 22600, deduced from DNA sequence
22000
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monomeric enzyme form, gel filtration
66000
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trimeric enzyme form, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
1 * 22600, deduced from DNA sequence
additional information
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enzyme is a monomer in solution, but crystallizes as a tightly packed trimer, in presence of stabilizing sulfate ions the monomeric and trimeric forms build an equilibrium in solution
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, purified enzyme in 20 mM Tris-HCl, pH 7.5, 0.2 M NaCl, 5% glycerol v/v, 10 mM DTT, from droplets of equal volume of protein solution and reservoir solution, reservoir solution: 20% w/v PEG 8K, 50 mM cacodylate, pH 6.5, 0.2 M (NH4)2SO4, 5% glycerol v/v, 21°C, within 4 days to 2 weeks, X-ray and light scattering structure determination and analysis
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from strain DL41
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene yacE, DNA sequence analysis
expressed in Escherichia coli strain BL21(DE3)
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gene coaE, expression as His-tagged protein in strain DL41
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. The DPCK method can be applied accurately to pools of CoA metabolites isolated from cellular material
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mishra, P.; Park, P.K.; Drueckhammer, D.G.
Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12
J. Bacteriol.
183
2774-2778
2001
Corynebacterium ammoniagenes, Escherichia coli (P0A6I9), Escherichia coli
Manually annotated by BRENDA team
O'Toole, N.; Barbosa, J.A.; Li, Y.; Hung, L.W.; Matte, A.; Cygler, M.
Crystal structure of a trimeric form of dephosphocoenzyme A kinase from Escherichia coli
Protein Sci.
12
327-336
2003
Escherichia coli
Manually annotated by BRENDA team
Wadler, C.; Cronan, J.E.
Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters
Anal. Biochem.
368
17-23
2007
Aquifex aeolicus, Escherichia coli, Homo sapiens
Manually annotated by BRENDA team