Information on EC 2.7.1.224 - cytidine diphosphoramidate kinase

for references in articles please use BRENDA:EC2.7.1.224

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

EC Tree 2 Transferases 2.7 Transferring phosphorus-containing groups 2.7.1 Phosphotransferases with an alcohol group as acceptor 2.7.1.224 cytidine diphosphoramidate kinase

IUBMB Comments

The enzyme, characterized from the bacterium Campylobacter jejuni, is involved in formation of a unique O-methyl phosphoramidate modification on specific sugar residues within the bacterium’s capsular polysaccharides.

The expected taxonomic range for this enzyme is: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

2.7.1.224
exonuclease
polynucleotide
pnpase
endoribonuclease
exonucleolytic
polyu
5'-mononucleotides
degradosome
dis3l2

Reaction Schemes

hide

show

ATP

cytidine 5'-diphosphoramidate

ADP

cytidine 3'-phospho-5'-diphosphoramidate

Synonyms
Cj1415c, more

top print hide

show all columns Go to Synonym Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Cj1415c

2 entries

Cj1415c

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

749926

Cj1415c

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

Q0P8J9

top print hide

show all columns Go to Reaction Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

ATP + cytidine 5'-diphosphoramidate = ADP + cytidine 3'-phospho-5'-diphosphoramidate

top print hide

Go to Systematic Name Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SYSTEMATIC NAME

IUBMB Comments

ATP:cytidine 5'-diphosphoramidate 3'-phosphotransferase

The enzyme, characterized from the bacterium Campylobacter jejuni, is involved in formation of a unique O-methyl phosphoramidate modification on specific sugar residues within the bacterium's capsular polysaccharides.

top print hide

show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

ATP + 2'-deoxycytidine 5'-diphosphoramidate

ADP + 2'-deoxycytidine 3'-phospho-5'-diphosphoramidate + H+

2 entries

ATP + adenosine 5'-diphosphoramidate

ADP + adenosine 3'-phospho-5'-diphosphoramidate + H+

2 entries

ATP + CDP-methyl phosphonate

ADP + ?

2 entries

ATP + cytidine 5'-diphosphoramidate

ADP + cytidine 3'-phospho-5'-diphosphoramidate + H+

2 entries

ATP + uridine 5'-diphosphoramidate

ADP + uridine 3'-phospho-5'-diphosphoramidate + H+

2 entries

ATP + 2'-deoxycytidine 5'-diphosphoramidate

ADP + 2'-deoxycytidine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

749926

Substrates: -
Products: -

ATP + 2'-deoxycytidine 5'-diphosphoramidate

ADP + 2'-deoxycytidine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

Q0P8J9

749926

Substrates: -
Products: -

ATP + adenosine 5'-diphosphoramidate

ADP + adenosine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

749926

Substrates: poor substrate
Products: -

ATP + adenosine 5'-diphosphoramidate

ADP + adenosine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

Q0P8J9

749926

Substrates: poor substrate
Products: -

ATP + CDP-methyl phosphonate

ADP + ?

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

749926

Substrates: -
Products: -

ATP + CDP-methyl phosphonate

ADP + ?

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

Q0P8J9

749926

Substrates: -
Products: -

ATP + cytidine 5'-diphosphoramidate

ADP + cytidine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

749926

Substrates: -
Products: -

ATP + cytidine 5'-diphosphoramidate

ADP + cytidine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

Q0P8J9

749926

Substrates: -
Products: -

ATP + uridine 5'-diphosphoramidate

ADP + uridine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

749926

Substrates: poor substrate
Products: -

ATP + uridine 5'-diphosphoramidate

ADP + uridine 3'-phospho-5'-diphosphoramidate + H+

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

Q0P8J9

749926

Substrates: poor substrate
Products: -

top print hide

show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.39

2'-deoxycytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

0.7 - 1.5

ATP

2 entries

0.8

CDP-methyl phosphonate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

0.11 - 4.4

cytidine 5'-diphosphoramidate

2 entries

0.7

ATP

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

mutant S85A, pH 8.0, 25°C

749926

1.5

ATP

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

0.11

cytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

4.4

cytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

mutant S85A, pH 8.0, 25°C

749926

top print hide

show all columns Go to Turnover Number Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2.2

2'-deoxycytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

1.6 - 2.2

ATP

2 entries

1.4

CDP-methyl phosphonate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

1.6 - 2.2

cytidine 5'-diphosphoramidate

2 entries

1.6

ATP

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

mutant S85A, pH 8.0, 25°C

749926

2.2

ATP

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

1.6

cytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

mutant S85A, pH 8.0, 25°C

749926

2.2

cytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

top print hide

show all columns Go to kcat/KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5.6

2'-deoxycytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

1.5 - 2.3

ATP

2 entries

1.7

CDP-methyl phosphonate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

0.36 - 20

cytidine 5'-diphosphoramidate

2 entries

1.5

ATP

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

2.3

ATP

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

mutant S85A, pH 8.0, 25°C

749926

0.36

cytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

mutant S85A, pH 8.0, 25°C

749926

cytidine 5'-diphosphoramidate

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

wild-type, pH 8.0, 25°C

749926

top print hide

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Campylobacter jejuni subsp. jejuni serotype O:2

749926

Q0P8J9

UniProt

brenda

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

749926

Q0P8J9

UniProt

brenda

top hide

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

top print hide

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

Q0P8J9 pBLAST

CDK_CAMJE

Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

170

19884

Swiss-Prot

Show Sequence

top print hide

show all columns Go to Protein Variants Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

S85A

2 entries

S85A

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

about 5% of wild-type catalytic efficiency

749926

S85A

Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819

about 5% of wild-type catalytic efficiency

top print hide

Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli

Campylobacter jejuni subsp. jejuni serotype O:2

Q0P8J9

749926

top print hide References Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

749926

Taylor, Z.W.; Raushel, F.M.

Cytidine diphosphoramidate kinase an enzyme required for the biosynthesis of the O-methyl phosphoramidate modification in the capsular polysaccharides of Campylobacter jejuni

Biochemistry

2238-2244

2018

Campylobacter jejuni subsp. jejuni serotype O:2 (Q0P8J9), Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 (Q0P8J9)

29578334

brenda

Entries 1 - 20 of 1

Show complete table

top print hide 10 entries

EXTERNAL LINKS (specific for EC-Number 2.7.1.224)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

html completed