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EC Tree
The taxonomic range for the selected organisms is: Homo sapiens The enzyme appears in selected viruses and cellular organisms
Synonyms
nicotinamide riboside kinase, nmrk2, nrk-2, ribosylnicotinamide kinase, nicotinamide riboside kinase 2, nmrk1, nrk2b, nicotinamide riboside kinase 1, nmr-k, nicotinamide ribose kinase,
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nicotinamide riboside kinase
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kinase, ribosylnicotinamide (phosphorylating)
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nicotinamide riboside kinase 1
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nicotinamide riboside kinase 2
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nicotinamide riboside kinase-2
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additional information
see also EC 2.7.1.173
additional information
see also EC 2.7.1.173
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phospho group transfer
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ATP:N-ribosylnicotinamide 5'-phosphotransferase
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ATP + 1-(beta-D-ribofuranosyl)-nicotinamide
ADP + beta-nicotinamide D-ribonucleotide
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GTP + 1-(beta-D-ribofuranosyl)-nicotinamide
GDP + beta-nicotinamide D-ribonucleotide
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additional information
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additional information
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enzyme NRK2 highly prefers ATP, while isozyme NRK1 also uses GTP with similar activity
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additional information
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enzyme NRK2 highly prefers ATP, while isozyme NRK1 also uses GTP with similar activity
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ATP + 1-(beta-D-ribofuranosyl)-nicotinamide
ADP + beta-nicotinamide D-ribonucleotide
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?
GTP + 1-(beta-D-ribofuranosyl)-nicotinamide
GDP + beta-nicotinamide D-ribonucleotide
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?
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Cardiomyopathy, Dilated
NMRK2 Gene Is Upregulated in Dilated Cardiomyopathy and Required for Cardiac Function and NAD Levels during Aging.
Glucose Intolerance
Tissue-specific regulation of sirtuin and nicotinamide adenine dinucleotide biosynthetic pathways identified in C57Bl/6 mice in response to high-fat feeding.
Heart Diseases
Nicotinamide riboside kinase-2 alleviates ischemia-induced heart failure through P38 signaling.
Heart Failure
Nicotinamide riboside kinase-2 alleviates ischemia-induced heart failure through P38 signaling.
ribosylnicotinamide kinase deficiency
Nicotinamide riboside kinase-2 alleviates ischemia-induced heart failure through P38 signaling.
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0.068 - 30
1-(beta-D-ribofuranosyl)-nicotinamide
0.068
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with GTP
0.088
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with ATP
0.19
1-(beta-D-ribofuranosyl)-nicotinamide
with ATP, pH and temperature not specified in the publication
30
1-(beta-D-ribofuranosyl)-nicotinamide
with GTP, pH and temperature not specified in the publication
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0.34 - 1.7
1-(beta-D-ribofuranosyl)-nicotinamide
0.34
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with GTP
0.6
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with ATP
0.75
1-(beta-D-ribofuranosyl)-nicotinamide
with ATP, pH and temperature not specified in the publication
1.7
1-(beta-D-ribofuranosyl)-nicotinamide
with GTP, pH and temperature not specified in the publication
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0.057 - 6.82
1-(beta-D-ribofuranosyl)-nicotinamide
0.057
1-(beta-D-ribofuranosyl)-nicotinamide
with GTP, pH and temperature not specified in the publication
3.95
1-(beta-D-ribofuranosyl)-nicotinamide
with ATP, pH and temperature not specified in the publication
5
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with GTP
6.82
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with ATP
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UniProt
brenda
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brenda
predominantly expresses in skeletal muscle with a trace amount expressed in healthy cardiac tissue
brenda
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and liver, highest eypression
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and kidney, highest eypression
brenda
predominantly expresses in skeletal muscle with a trace amount expressed in healthy cardiac tissue
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additional information
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presence of quinolinate phosphoribosyltransferase and nicotinamide riboside kinase in all examined tissues/cell lines
brenda
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malfunction
NRK-2 plays a critical role in heart failure progression following ischemic injury. NRK-2 deficiency promotes post-MI scar expansion, rapid LV chamber dilatation, cardiac dysfunction and fibrosis possibly due to increased p38slphs activation
metabolism
the enzyme is involved in the NAD+ biosynthesis pathway. In the initial step of the pathway, NRK activity catalyses the phosphorylation of nicotinamide riboside (NR) to nicotinamide mononucleotide (NMN). Importance of different salvage pathways involved in metabolising the vitamin B3 class of NAD+ precursor molecules, with a particular focus on the nicotinamide riboside kinase pathway at both a tissue-specific and systemic level, regulation of the NRK enzymes, overview. Alternatively, NRK activity can phosphorylate nicotinic acid riboside (NaR) to nicotinic acid mononucleotide (NaMN), see for EC 2.7.1.173
physiological function
NRK-2 plays a critical role in heart failure progression following ischemic injury. NRK-2 deficiency promotes post-MI scar expansion, rapid LV chamber dilatation, cardiac dysfunction and fibrosis possibly due to increased p38slphs activation
physiological function
NRK2 appears to play a redundant role in NAD+ biosynthesis along with NRK1, at least in unchallenged models, its highly regulated expression particularly in times of stress suggest it may have role beyond NAD+ metabolism
additional information
proposed NRK expression in disease and potential therapeutic interventions
additional information
proposed NRK expression in disease and potential therapeutic interventions
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PLAT2_HUMAN
162
1
17394
Swiss-Prot
other Location (Reliability: 3 )
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sitting-drop vapor-diffusion method at 22°C, crystal structures of NRK1 in a binary complex with the reaction product nicotinamide mononucleotide at 1.5 A resolution and in a ternary complex with ADP and tiazofurin at 2.7 A resolution. The active site is located in a groove between the central parallel beta sheet core and the LID and NMP-binding domains
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D138A
mutation has minor effects on catalysis
D36A
mutation abolishes activity
D56A
mutation abolishes activity
K16A
mutation abolishes activity
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expression in Escherichia coli
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Nmrk2 mRNA expression is substantially induced (over 80fold) in models of lethal cardiomyopathy
NRK-2 expression dramatically increases in ischemic heart
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analysis
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simple, fast and sensitive coupled fluorometric assay that enables simultaneous determination of nicotinamide phosphoribosyltransferase EC 2.4.2.12, quinolinate phosphoribosyltransferase EC 2.4.2.19, nicotinate phosphoribosyltransferase EC 6.3.4.21 and nicotinamide riboside kinase in whole-cell extracts and biological fluids yielding an overall picture of the tissue/cell-specific distribution of the activities of the various enzymes
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Khan, J.A.; Xiang, S.; Tong, L.
Crystal structure of human nicotinamide riboside kinase
Structure
15
1005-1013
2007
Homo sapiens (Q9NWW9), Homo sapiens
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Zamporlini, F.; Ruggieri, S.; Mazzola, F.; Amici, A.; Orsomando, G.; Raffaelli, N.
Novel assay for simultaneous measurement of pyridine mononucleotides synthesizing activities allows dissection of the NAD+ biosynthetic machinery in mammalian cells
FEBS J.
281
5104-5119
2014
Homo sapiens
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Ahmad, F.; Tomar, D.; Aryal A C, S.; Elmoselhi, A.B.; Thomas, M.; Elrod, J.W.; Tilley, D.G.; Force, T.
Nicotinamide riboside kinase-2 alleviates ischemia-induced heart failure through P38 signaling
Biochim. Biophys. Acta
1866
165609
2020
Mus musculus (Q9D7C9), Mus musculus, Homo sapiens (Q9NPI5), Homo sapiens, Mus musculus C57BL/6N (Q9D7C9)
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Fletcher, R.S.; Lavery, G.G.
The emergence of the nicotinamide riboside kinases in the regulation of NAD+ metabolism
J. Mol. Endocrinol.
61
R107-R121
2018
Danio rerio (F1QSN9), Mus musculus (Q91W63), Mus musculus (Q9D7C9), Homo sapiens (Q9NPI5), Homo sapiens (Q9NWW6)
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