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Information on EC 2.7.1.213 - cytidine kinase

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EC Tree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.213 cytidine kinase

IUBMB Comments

The enzyme, characterized from the archaeon Thermococcus kodakarensis, participates in a pathway for nucleoside degradation. The enzyme can also act on deoxycytidine and uridine, but unlike EC 2.7.1.48, uridine kinase, it is most active with cytidine.

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2.7.1.213
uridine
nucleoside
monophosphate
pyrimidine
triphosphate
salvage
uridine-cytidine
deoxycytidine

The expected taxonomic range for this enzyme is: Archaea, Eukaryota

Reaction Schemes

Synonyms

TK1843,

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

TK1843

Thermococcus kodakarensis

Q5JEK6

locus name

734747

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

ATP + cytidine = ADP + CMP

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Go to Pathway Search

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PATHWAY SOURCE

PATHWAYS

KEGG

Pyrimidine metabolism

, Pyrimidine metabolism

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SYSTEMATIC NAME

IUBMB Comments

ATP:cytidine 5'-phosphotransferase

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

3'-deoxycytidine + ATP

3'-dCMP + ADP

Thermococcus kodakarensis

Q5JEK6

734747

cytidine + ATP

CMP + ADP

2 entries

cytidine + CTP

CMP + CDP

2 entries

cytidine + GTP

CMP + GDP

2 entries

cytidine + UTP

CMP + UDP

2 entries

uridine + ATP

UMP + ADP

Thermococcus kodakarensis

Q5JEK6

734747

additional information

Schistosoma mansoni

cytidine kinase is specific for cytidine, but various nucleoside 5'-triphosphate act as phosphate donors for the kinase albeit to different degrees, overview. Poor activity with dTTP, no activity with AMP and 4-nitrophenyl phosphate

739025

cytidine + ATP

CMP + ADP

Schistosoma mansoni

739025

cytidine + ATP

CMP + ADP

Thermococcus kodakarensis

Q5JEK6

734747

cytidine + CTP

CMP + CDP

Schistosoma mansoni

739025

cytidine + CTP

CMP + CDP

Thermococcus kodakarensis

Q5JEK6

30% activity with CTP compared to ATP

734747

cytidine + GTP

CMP + GDP

Schistosoma mansoni

preferred cofactor

739025

cytidine + GTP

CMP + GDP

Thermococcus kodakarensis

Q5JEK6

70% activity with GTP compared to ATP

734747

cytidine + UTP

CMP + UDP

Schistosoma mansoni

739025

cytidine + UTP

CMP + UDP

Thermococcus kodakarensis

Q5JEK6

30% activity with UTP compared to ATP

734747

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

cytidine + ATP

CMP + ADP

Thermococcus kodakarensis

Q5JEK6

734747

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

ATP

CTP

GTP

preferred cofactor

UTP

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Co2+

Thermococcus kodakarensis

Q5JEK6

26% activity compared to Mg2+

734747

KCl

Thermococcus kodakarensis

Q5JEK6

300 mM, 3.6fold increase of activity

734747

Mg2+

2 entries

Mn2+

Thermococcus kodakarensis

Q5JEK6

68% activity compared to Mg2+

734747

additional information

Thermococcus kodakarensis

Q5JEK6

ca2+, Ni2+, Zn2+, cu2+ show less than 20% avtivation

734747

Mg2+

Schistosoma mansoni

required

739025

Mg2+

Thermococcus kodakarensis

Q5JEK6

most preferred divalent metal ion

734747

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

cytidine

Thermococcus kodakarensis

Q5JEK6

substrate inhibition

734747

uridine

Schistosoma mansoni

competitive inhibition

739025

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DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

cytidine kinase deficiency

ddC- and 3TC-bis(SATE) monophosphate prodrugs overcome cellular resistance mechanisms to HIV-1 associated with cytidine kinase deficiency.

10432710

Infections

Ehrlichia chaffeensis replication sites in adult Drosophila melanogaster.

23306065

Neoplasms

Uridine Cytidine Kinase Like-1 Enhances Tumor Cell Proliferation and Mediates Protection from Natural Killer-Mediated Killing.

32083188

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

4.11

3'-deoxycytidine

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

2.1 - 5.72

ATP

2 entries

0.073 - 1.71

cytidine

2 entries

2.1

ATP

Schistosoma mansoni

pH 7.4, 20-40°C

739025

5.72

ATP

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

0.073

cytidine

Schistosoma mansoni

pH 7.4, 20-40°C, with ATP

739025

1.71

cytidine

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

226

3'-deoxycytidine

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

222

ATP

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

280

cytidine

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

3'-deoxycytidine

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

38.8

ATP

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

164

cytidine

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

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Ki VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

29.3

cytidine

Thermococcus kodakarensis

Q5JEK6

pH and temperature not specified in the publication

734747

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

7.4

Schistosoma mansoni

assay at

739025

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5 - 9

Thermococcus kodakarensis

Q5JEK6

734747

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

20 - 40

Schistosoma mansoni

assay at

739025

Thermococcus kodakarensis

Q5JEK6

734747

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Schistosoma mansoni

739025

brenda

Thermococcus kodakarensis

734747

Q5JEK6

UniProt

brenda

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

Q57849 pBLAST

NK_METJA

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

302

33920

Swiss-Prot

Show Sequence

Q9HJT3 pBLAST

NK_THEAC

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

287

32805

Swiss-Prot

Show Sequence

Q5JEK6 pBLAST

CYDK_THEKO

Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

278

30419

Swiss-Prot

Show Sequence

A0A7G9YZU7 pBLAST

A0A7G9YZU7_9EURY

Methanosarcinales archaeon ANME-1 ERB6

293

33105

TrEMBL

Show Sequence

A0A7G9Z4P8 pBLAST

A0A7G9Z4P8_9EURY

Methanosarcinales archaeon ANME-1 ERB7

324

36630

TrEMBL

Show Sequence

A0A7G9ZC68 pBLAST

A0A7G9ZC68_9EURY

Methanosarcinales archaeon ANME-1 ERB7

319

35833

TrEMBL

Show Sequence

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

upon freezing at -20°C all activity is lost from the cytosol within a few hours, addition of PMSF preserved kinase activities for a week at 4°C and well over 3 weeks at -80°C

Schistosoma mansoni

739025

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

partially, separation of cytosolic cytidine and uridine kinases by anion exchange chromatography

Schistosoma mansoni

739025