We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme, characterized from the archaeon Thermococcus kodakarensis, participates in a pathway for nucleoside degradation. The enzyme can also act on deoxycytidine and uridine, but unlike EC 2.7.1.48, uridine kinase, it is most active with cytidine.
The expected taxonomic range for this enzyme is: Archaea, Eukaryota
Synonyms
TK1843,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + cytidine = ADP + CMP
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP:cytidine 5'-phosphotransferase
The enzyme, characterized from the archaeon Thermococcus kodakarensis, participates in a pathway for nucleoside degradation. The enzyme can also act on deoxycytidine and uridine, but unlike EC 2.7.1.48, uridine kinase, it is most active with cytidine.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3'-deoxycytidine + ATP
3'-dCMP + ADP
-
-
-
?
uridine + ATP
UMP + ADP
-
-
-
?
additional information
?
-
-
cytidine kinase is specific for cytidine, but various nucleoside 5'-triphosphate act as phosphate donors for the kinase albeit to different degrees, overview. Poor activity with dTTP, no activity with AMP and 4-nitrophenyl phosphate
-
-
?
cytidine + ATP
CMP + ADP
-
-
-
-
?
cytidine + ATP
CMP + ADP
-
-
-
?
cytidine + CTP
CMP + CDP
-
-
-
-
?
cytidine + CTP
CMP + CDP
30% activity with CTP compared to ATP
-
-
?
cytidine + GTP
CMP + GDP
-
preferred cofactor
-
-
?
cytidine + GTP
CMP + GDP
70% activity with GTP compared to ATP
-
-
?
cytidine + UTP
CMP + UDP
-
-
-
-
?
cytidine + UTP
CMP + UDP
30% activity with UTP compared to ATP
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cytidine + ATP
CMP + ADP
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Co2+
26% activity compared to Mg2+
KCl
300 mM, 3.6fold increase of activity
Mn2+
68% activity compared to Mg2+
additional information
ca2+, Ni2+, Zn2+, cu2+ show less than 20% avtivation
Mg2+
-
required
Mg2+
most preferred divalent metal ion
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cytidine
substrate inhibition
uridine
-
competitive inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cytidine kinase deficiency
ddC- and 3TC-bis(SATE) monophosphate prodrugs overcome cellular resistance mechanisms to HIV-1 associated with cytidine kinase deficiency.
Infections
Ehrlichia chaffeensis replication sites in adult Drosophila melanogaster.
Neoplasms
Uridine Cytidine Kinase Like-1 Enhances Tumor Cell Proliferation and Mediates Protection from Natural Killer-Mediated Killing.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.11
3'-deoxycytidine
pH and temperature not specified in the publication
2.1
ATP
-
pH 7.4, 20-40°C
5.72
ATP
pH and temperature not specified in the publication
0.073
cytidine
-
pH 7.4, 20-40°C, with ATP
1.71
cytidine
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
226
3'-deoxycytidine
pH and temperature not specified in the publication
222
ATP
pH and temperature not specified in the publication
280
cytidine
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
55
3'-deoxycytidine
pH and temperature not specified in the publication
38.8
ATP
pH and temperature not specified in the publication
164
cytidine
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
29.3
cytidine
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CYDK_THEKO
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
278
0
30419
Swiss-Prot
-
NK_METJA
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
302
0
33920
Swiss-Prot
-
NK_THEAC
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
287
0
32805
Swiss-Prot
-
A0A7G9ZC68_9EURY
319
0
35833
TrEMBL
-
A0A7G9YZU7_9EURY
293
0
33105
TrEMBL
-
A0A7G9Z4P8_9EURY
324
0
36630
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
upon freezing at -20°C all activity is lost from the cytosol within a few hours, addition of PMSF preserved kinase activities for a week at 4°C and well over 3 weeks at -80°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
partially, separation of cytosolic cytidine and uridine kinases by anion exchange chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Aono, R.; Sato, T.; Imanaka, T.; Atomi, H.
A pentose bisphosphate pathway for nucleoside degradation in Archaea
Nat. Chem. Biol.
11
355-360
2015
Thermococcus kodakarensis (Q5JEK6)
brenda
Naguib, F.N.; El Kouni, M.H.
Nucleoside kinases in adult Schistosoma mansoni: phosphorylation of pyrimidine nucleosides
Mol. Biochem. Parasitol.
194
53-55
2014
Schistosoma mansoni
brenda
Select items on the left to see more content.
html completed