Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.1.20 - adenosine kinase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LZG0

for references in articles please use BRENDA:EC2.7.1.20
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
2-Aminoadenosine can also act as acceptor.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9LZG0
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
hide
ATP
+
adenosine
=
ADP
+
AMP
+
=
+
Synonyms
adenosine kinase, adk, ado kinase, adk-l, adk-s, rv2202c, atp:adenosine 5'-phosphotransferase, ldadk, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adenosine kinase (phosphorylating)
-
-
-
-
kinase, adenosine (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:adenosine 5'-phosphotransferase
2-Aminoadenosine can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-72-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + adenosine
ADP + AMP
show the reaction diagram
ATP + adenosine
ADP + AMP
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + adenosine
ADP + AMP
show the reaction diagram
the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP
-
-
?
ATP + adenosine
ADP + AMP
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
divalent cation requirement
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
geminivirus AL2
inactivation of the enzyme in vitro and in vivo via direct interaction, AL2 is a RNA geminivirus of the Begomovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview
-
geminivirus L2
inactivation of the enzyme in vitro and in vivo via direct interaction, L2 is a RNA geminivirus of the Curtovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview
-
additional information
-
enzyme activity decreases by 5.5fold in senescent cell cultures, apoptosis does not influence enzyme levels
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the activity of both isozymes is increased by 1.5-3.5fold in case of increased methylation activity, e.g. during higher cell wall synthesis rates, apoptosis does not influence enzyme levels
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
2 isozymes ADK1 and ADK2
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
enzyme activity decreases by 5.5fold in senescent cell cultures, 1.5-3.5fold increased activity of both isozymes during cell wall synthesis
Manually annotated by BRENDA team
-
high expression level of both isozymes
Manually annotated by BRENDA team
-
high expression level of both isozymes
Manually annotated by BRENDA team
-
highest expression level of both isozymes
Manually annotated by BRENDA team
-
expanding green siliques contain a 4fold higher expression of isozyme ADK1 compared to isozyme ADK2
Manually annotated by BRENDA team
additional information
-
tissue expression level analysis, the isozymes have similar expression patterns, except in case of microgametogenesis, where isozyme ADK1 is upregulated while isozyme ADK2 is not, ADK1 is the major isozyme
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
silencing of adenosine kinase leads to impaired root growth, small, crinkled rosette leaves, and reduced apical dominance. ADK-deficient tissues exhibit increased Arabidopsis Response Regulator5 and Arabidopsis Response Regulator7 expression
physiological function
-
adenosine kinase contributes to cytokinin homeostasis in vivo
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ADK2_ARATH
345
0
37846
Swiss-Prot
other Location (Reliability: 3)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
the active form of the enzyme is a monomer, which is composed of an alphabetaalpha sandwich domain and a smaller lid domain
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
ADK gene silencing induces the expression of S-adenosyl-L-homocysteine hydrolase resulting in up to 40fold higher S-adenosyl-L-homocysteine levels compared to wild-type plants, completely enzyme-deficient insertion mutants cannot be recovered, while mutants showing less than 10% residual activity are viable with altered phenotypes including small, rounded and wavy leaves and delayed senescence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partially, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, the His-tagged enzyme is expressed with or without coexpression of viral AL2 and L2 proteins in an enzyme-deficient Escherichia coli strain, enzyme expression leads to complementation of the mutant, coexpression leads to inactivation of the enzyme and no complementation, expression in yeast cells utilizing the two-hybrid system coexpressing the L2 protein of geminivirus, coexpression leads to inactivation of the enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schoor, S.; Moffatt, B.A.
Applying high throughput techniques in the study of adenosine kinase in plant metabolism and development
Front. Biosci.
9
1771-1781
2004
Arabidopsis thaliana, Avicennia marina, Solanum lycopersicum, Medicago truncatula, Nicotiana tabacum, Physcomitrium patens, Populus sp., Spinacia oleracea
Manually annotated by BRENDA team
Wang, H.; Hao, L.; Shung, C.Y.; Sunter, G.; Bisaro, D.M.
Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins
Plant Cell
15
3020-3032
2003
Arabidopsis thaliana (Q9LZG0), Nicotiana benthamiana
Manually annotated by BRENDA team
Young, L.S.; Harrison, B.R.; Narayana Murthy, U.M.; Moffatt, B.A.; Gilroy, S.; Masson, P.H.
Adenosine kinase modulates root gravitropism and cap morphogenesis in Arabidopsis
Plant Physiol.
142
564-573
2006
Arabidopsis thaliana (Q9SF85)
Manually annotated by BRENDA team
Park, J.; Gupta, R.S.
Adenosine kinase and ribokinase - the RK family of proteins
Cell. Mol. Life Sci.
65
2875-2896
2008
Arabidopsis thaliana, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Leishmania donovani, Schizosaccharomyces pombe, Spinacia oleracea, Trypanosoma brucei, Homo sapiens (P55263)
Manually annotated by BRENDA team
Schoor, S.; Farrow, S.; Blaschke, H.; Lee, S.; Perry, G.; von Schwartzenberg, K.; Emery, N.; Moffatt, B.
Adenosine kinase contributes to cytokinin interconversion in Arabidopsis
Plant Physiol.
157
659-672
2011
Arabidopsis thaliana
Manually annotated by BRENDA team