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Information on EC 2.7.1.20 - adenosine kinase and Organism(s) Mus musculus and UniProt Accession P55264
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2.7.1.20 adenosine kinaseIUBMB Comments
2-Aminoadenosine can also act as acceptor.
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Word Map
- 2.7.1.20
- nucleoside
- deaminase
- purine
- amp
- adenylate
- inosine
-
salvage
- hypoxanthine
- epilepsy
-
phosphoribosyltransferase
- 5'-nucleotidase
- 5-iodotubercidin
- riboside
- deoxyadenosine
- s-adenosylhomocysteine
- deoxycytidine
- aicar
-
anticonvulsant
- tubercidin
- dipyridamole
-
neuromodulator
- ehna
-
2\'-deoxycoformycin
-
ecto-5\'-nucleotidase
- ribokinase
-
erythro-9-2-hydroxy-3-nonyl
-
adenosinergic
- 6-methylmercaptopurine
-
3hadenosine
- 2-chloroadenosine
- coformycin
-
nitrobenzylthioinosine
-
epileptogenesis
-
hypoxanthine-guanine
-
transmethylation
-
adenosine-induced
- hgprt
-
kinase-deficient
- 2'-deoxyadenosine
-
adenosine-mediated
-
3.5.4.4
- deoxycoformycin
-
non-nucleoside
- analysis
-
2.4.2.8
- 9-beta-d-arabinofuranosyladenine
-
n6-cyclopentyladenosine
-
erythro-9-2-hydroxy-3-nonyladenine
-
dado
- ara-a
- medicine
- diagnostics
-
rephosphorylation
- pharmacology
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
adenosine kinase, adk, ado kinase, adk-l, adk-s, rv2202c, atp:adenosine 5'-phosphotransferase, ldadk, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
adenosine kinase (phosphorylating)
-
-
-
-
kinase, adenosine (phosphorylating)
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:adenosine 5'-phosphotransferase
2-Aminoadenosine can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY
9027-72-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 6-methylmercaptopurine riboside
ADP + 6-methylmercaptopurine-D-riboside 5'-phosphate
-
-
-
-
?
additional information
?
-
-
low levels of ADK are essential to maintain adenosine-mediated neuroprotection
-
-
?
ATP + adenosine
ADP + AMP
the major route of myocardial adenosine metabolism. Receptor-independent role of adenosine for adenosine kinase-mediated adenosine metabolism in cardiomyocyte microtubule dynamics and protection against maladaptive hypertrophy
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
low levels of ADK are essential to maintain adenosine-mediated neuroprotection
-
-
?
ATP + adenosine
ADP + AMP
the major route of myocardial adenosine metabolism. Receptor-independent role of adenosine for adenosine kinase-mediated adenosine metabolism in cardiomyocyte microtubule dynamics and protection against maladaptive hypertrophy
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K+
allosteric activator, it increases the catalytic turnover (Kcat) but does not affect the affinity of the enzyme for adenosine or ATP
Co2+
Fe2+
Mg2+
Mn2+
Ni2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5'-amino-5'-deoxy-adenosine
-
following systemic administration, adenosine kinase inhibitors can alleviate acute thermal nociception as measured by the hot-plate test in mice
5'-deoxy-5-iodotubercidin
-
following systemic administration, adenosine kinase inhibitors can alleviate acute thermal nociception as measured by the hot-plate test in mice
5-iodotubercidin
-
following systemic administration, adenosine kinase inhibitors can alleviate acute thermal nociception as measured by the hot-plate test in mice
6-methylmercaptopurine riboside phosphate
-
product inhibition, competitive with respect to ATP and noncompetitive with respect to 6-methylmercaptopurine ribonucleoside
7-beta-D-ribofuranosyl-pyrrolo-(2,3-d)-pyrimidine
-
competitive with respect to 6-methylmercaptopurine riboside and noncompetitive with respect to ATP
ADP
-
noncompetitive with respect to ATP and 6-methylmercaptopurine ribonucleoside at low concentrations of the substrates
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozymes AK-L and AK-S; a long and a short isozyme, i.e. AK-L and AK-S
SwissProt
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
ADK expression in young neurons may provide a salvage pathway to utilize adenosine in nucleic acid synthesis
additional information
-
astrocyte-based ADK provides a critical link between astrogliosis and neuronal dysfunction in epilepsy
-
at early postnatal stages ADK immunoreactivity is prominent in neurons, notably in cerebral cortex and hippocampus. Thereafter, adenosine kinase gradually disappears from neurons and appears in newly developed nestin- and glial fibrillary acidic protein-positive astrocytes. The region-specific downregulation of neuronal adenosine kinase coincides with the onset of myelination. After postnatal day 14, the transition from neuronal to astrocytic ADK expression is complete, except in a subset of neurons that retain ADK until adulthood in specific regions, such as the striatum
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low levels of adenosine kinase are essential to maintain adenosine-mediated endogenous neuroprotection and pathologic increases in ADK may render the brain more susceptible to injury
-
transgenic overexpression of adenosine kinase in brain leads to multiple learning impairments and altered sensitivity to psychomimetic drugs
-
transgenic overexpression of ADK, which reduces adenosine in mouse brain, results in increased levels of myelin proteolipid protein
-
transient downregulation of hippocampal ADK after stroke might be a protective mechanism during maturation hippocampal cell loss
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
disruption of adenosine kinase causes spontaneous cardiac hypertrophy
metabolism
the enzyme is involved in myocardial adenosine metabolism. Cardiomyocyte adenosine kinase expression attenuates pressure overload induced hypertrophy. Aadenosine kinase regulates cardiac mTORC1 and p44/42 ERK MAP kinase signaling. Adenosine metabolism by adenosine kinase diminishes detyrosinated microtubules
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ADK_MOUSE
361
0
40149
Swiss-Prot
other Location (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
43500
x * 45000, recombinant His-tagged isozyme AK-L, SDS-PAGE, x * 43500, recombinant His-tagged isozyme AK-S, SDS-PAGE
45000
x * 45000, recombinant His-tagged isozyme AK-L, SDS-PAGE, x * 43500, recombinant His-tagged isozyme AK-S, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 45000, recombinant His-tagged isozyme AK-L, SDS-PAGE, x * 43500, recombinant His-tagged isozyme AK-S, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
the recombinant isozymes AK-L and AK-S became phosphorylated by protein kinase C, no significant phosphorylation level by protein kinases PKA, PKG, MAPK, CK2, and Cdk1, overview
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, crystal structures of binary complex with adenosine at 1.2 A and of ternary complex with ADP and adenosine at 1.8 A
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged isozymes AK-L and AK-S from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
streptomycin sulfate and ammonium sulfate fractionation, chromatography on DEAE-cellulose and affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isozymes AK-L and AK-S, DNA and amino acid sequence determination and analysis, overexpression of His-tagged proteins in Escherichia coli strain BL21(DE3)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
medicine
-
the enzyme is a rational therapeutic target to enhance neuroprotection in ischemia
medicine
-
astrocyte-based ADK provides a critical link between astrogliosis and neuronal dysfunction in epilepsy
medicine
-
pathologic overexpression of ADK as in epilepsy may also render the brain more susceptible to injury from ischemia. Consequently, ADK emerges as a rational therapeutic target to enhance neuroprotection
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Divekar, A.Y.; Hakala, M.T.; Chheda, G.B.; Hong, C.I.
6-Ureido derivatives of 9-(-D-ribofuranosyl)purine as inhibitors of adenosine kinase
Biochem. Pharmacol.
22
545-548
1973
Mus musculus
Lindberg, B.; Klenow, H.; Hansen, K.
Some properties of partially purified mammalian adenosine kinase
J. Biol. Chem.
242
350-356
1967
Oryctolagus cuniculus, Mus musculus
Chang, C.H.; Brockman, R.W.; Bennett, L.L.
Adenosine kinase from L1210 cells. Purification and some properties of the enzyme
J. Biol. Chem.
255
2366-2371
1980
Mus musculus
Henderson, J.F.; Mikoshiba, A.; Chu, S.Y.; Caldwell, I.C.
Kinetic studies of adenosine kinase from Ehrlich ascites tumor cells
J. Biol. Chem.
247
1972-1975
1972
Mus musculus
Sahin, B.; Kansy, J.W.; Nairn, A.C.; Spychala, J.; Ealick, S.E.; Fienberg, A.A.; Greene, R.W.; Bibb, J.A.
Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation
Eur. J. Biochem.
271
3547-3555
2004
Mus musculus (P55264), Mus musculus
Matulenko, M.A.; Paight, E.S.; Frey, R.R.; Gomtsyan, A.; DiDomenico, S.; Jiang, M.; Lee, C.H.; Stewart, A.O.; Yu, H.; Kohlhaas, K.L.; Alexander, K.M.; McGaraughty, S.; Mikusa, J.; Marsh, K.C.; Muchmore, S.W.; Jakob, C.L.; Kowaluk, E.A.; Jarvis, M.F.; Bhagwat, S.S.
4-amino-5-aryl-6-arylethynylpyrimidines: structure-activity relationships of non-nucleoside adenosine kinase inhibitors
Bioorg. Med. Chem.
15
1586-1605
2007
Mus musculus
Pignataro, G.; Maysami, S.; Studer, F.E.; Wilz, A.; Simon, R.P.; Boison, D.
Downregulation of hippocampal adenosine kinase after focal ischemia as potential endogenous neuroprotective mechanism
J. Cereb. Blood Flow Metab.
28
17-23
2007
Mus musculus
Studer, F.E.; Fedele, D.E.; Marowsky, A.; Schwerdel, C.; Wernli, K.; Vogt, K.; Fritschy, J.M.; Boison, D.
Shift of adenosine kinase expression from neurons to astrocytes during postnatal development suggests dual functionality of the enzyme
Neuroscience
142
125-137
2006
Mus musculus
Yee, B.K.; Singer, P.; Chen, J.F.; Feldon, J.; Boison, D.
Transgenic overexpression of adenosine kinase in brain leads to multiple learning impairments and altered sensitivity to psychomimetic drugs
Eur. J. Neurosci.
26
3237-3252
2007
Mus musculus
Pignataro, G.; Simon, R.P.; Boison, D.
Transgenic overexpression of adenosine kinase aggravates cell death in ischemia
J. Cereb. Blood Flow Metab.
27
1-5
2007
Mus musculus
Li, T.; Ren, G.; Lusardi, T.; Wilz, A.; Lan, J.Q.; Iwasato, T.; Itohara, S.; Simon, R.P.; Boison, D.
Adenosine kinase is a target for the prediction and prevention of epileptogenesis in mice
J. Clin. Invest.
118
571-582
2008
Mus musculus
Wu, N.L.; Boison, D.
Adenosine kinase expression modulates expression of myelin proteolipid protein
Open Neurosci. J.
1
15-19
2007
Mus musculus
-
Fassett, J.; Xu, X.; Kwak, D.; Zhu, G.; Fassett, E.K.; Zhang, P.; Wang, H.; Mayer, B.; Bache, R.J.; Chen, Y.
Adenosine kinase attenuates cardiomyocyte microtubule stabilization and protects against pressure overload-induced hypertrophy and LV dysfunction
J. Mol. Cell. Cardiol.
130
49-58
2019
Mus musculus (P55264)
de Oliveira, R.R.; Morales-Neto, R.; Rocco, S.A.; Sforca, M.L.; Polo, C.C.; Tonoli, C.C.C.; Mercaldi, G.F.; Cordeiro, A.T.; Murakami, M.T.; Franchini, K.G.
Adenosine kinase couples sensing of cellular potassium depletion to purine metabolism
Sci. Rep.
8
11988
2018
Mus musculus (P55264), Mus musculus
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