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Information on EC 2.7.1.2 - glucokinase and Organism(s) Streptomyces griseus and UniProt Accession B1VZT1

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IUBMB Comments
A group of enzymes found in invertebrates and microorganisms that are highly specific for glucose.
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Streptomyces griseus
UNIPROT: B1VZT1
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The taxonomic range for the selected organisms is: Streptomyces griseus
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
glucokinase, atp-dependent glucokinase, tcglck, atp-glk, sgglka, tte0090, klglk1, sw2155, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucokinase (phosphorylating)
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kinase, gluco- (phosphorylating)
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-glucose 6-phosphotransferase
A group of enzymes found in invertebrates and microorganisms that are highly specific for glucose.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-36-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
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?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
SgGlkA is divided into a small alpha/beta domain and a large alpha + beta domain, and it forms a dimer-of-dimer tetrameric configuration
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structures of apo-SgGlkA, SgGlkA in complex with glucose, and SgGlkA in complex with glucose and adenylyl imidodiphosphate (AMPPNP) are reported. SgGlkA is divided into a small alpha/beta domain and a large alpha + beta domain, and it forms a dimer-of-dimer tetrameric configuration
crystallized using the sitting-drop vapour-diffusion method. A crystal of SgGlkA in complex with glucose is obtained and diffracted X-rays to 1.84 A resolution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Miyazono, K.; Tabei, N.; Marushima, K.; Ohnishi, Y.; Horinouchi, S.; Tanokura, M.
Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose
Acta Crystallogr. Sect. F
67
914-916
2011
Streptomyces griseus (B1VZT1), Streptomyces griseus
Manually annotated by BRENDA team
Miyazono, K.; Tabei, N.; Morita, S.; Ohnishi, Y.; Horinouchi, S.; Tanokura, M.
Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus
J. Bacteriol.
194
607-616
2012
Streptomyces griseus (B1VZT1), Streptomyces griseus
Manually annotated by BRENDA team