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Information on EC 2.7.1.189 - autoinducer-2 kinase
for references in articles please use BRENDA:EC2.7.1.189
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EC Tree 2 Transferases
2.7 Transferring phosphorus-containing groups
2.7.1 Phosphotransferases with an alcohol group as acceptor
2.7.1.189 autoinducer-2 kinase
IUBMB Comments
The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2.
The enzyme appears in viruses and cellular organisms
- 2.7.1.189
-
quorum
-
cell-cell
-
interspecies
- repressor
-
chemotaxis
- amp
-
prevailing
- typhimurium
-
envision
-
quorum-sensing
-
fight
- drug development
Reaction Schemes
showSynonyms
ai-2 kinase, autoinducer-2 kinase, lsrk kinase, ai-2 processing kinase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AI-2 kinase
lsrK
LsrK kinase
lsrK
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + (S)-4,5-dihydroxypentane-2,3-dione = ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
ATP + (S)-4,5-dihydroxypentane-2,3-dione = ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
-
-
-
-
ATP + (S)-4,5-dihydroxypentane-2,3-dione = ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
apid equilibrium ordered mechanism with ATP binding first
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
autoinducer AI-2 degradation
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SYSTEMATIC NAME
IUBMB Comments
ATP:(S)-4,5-dihydroxypentane-2,3-dione 5-phosphotransferase
The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (4S)-4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates C5 of the open form of the substrate
Products: -
Products: -
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxydecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxodecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxydecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxodecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxydecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxodecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
Escherichia coli W3110 / ATCC 27325
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
Escherichia coli W3110 / ATCC 27325
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxynonane-3,4-dione
ADP + 2-hydroxy-3,4-dioxononyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxynonane-3,4-dione
ADP + 2-hydroxy-3,4-dioxononyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxynonane-3,4-dione
ADP + 2-hydroxy-3,4-dioxononyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyundecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoundecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyundecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoundecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyundecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoundecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
Escherichia coli W3110 / ATCC 27325
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, mass spectrometric product identification. Molecule (S)-4,5-dihydroxypentane-2,3-dione (DPD) is the precursor of the signal molecule autoinducer-2
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates C5 of the open form of the substrate
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
Escherichia coli W3110 / ATCC 27325
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: no phosphorylation of deoxy-AI-2-analogues by LsrK. Synthesis of substrate analogues and activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview
Products: -
Products: -
?
additional information
?
-
Escherichia coli W3110 / ATCC 27325
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 1,2-dihydroxy-6-methylheptane-3,4-dione
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: no phosphorylation of deoxy-AI-2-analogues by LsrK. Synthesis of substrate analogues and activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview
Products: -
Products: -
?
additional information
?
-
Substrates: (S)-4,5-dihydroxypentane-2,3-dione (DPD) docking results show that the C5 position of DPD, where the phosphorylation occurs, orients toward ATP by forming interactions with Thr21
Products: -
Products: -
?
additional information
?
-
Substrates: (S)-4,5-dihydroxypentane-2,3-dione (DPD) docking results show that the C5 position of DPD, where the phosphorylation occurs, orients toward ATP by forming interactions with Thr21
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
Substrates: (S)-4,5-dihydroxypentane-2,3-dione (DPD) docking results show that the C5 position of DPD, where the phosphorylation occurs, orients toward ATP by forming interactions with Thr21
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: no phosphorylation of deoxy-AI-2-analogues by LsrK. Synthesis of substrate analogues and activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (4S)-4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates C5 of the open form of the substrate
Products: -
Products: -
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + (S)-4,5-dihydroxypentane-2,3-dione
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxydecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxodecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxydecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxodecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxynonane-3,4-dione
ADP + 2-hydroxy-3,4-dioxononyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxynonane-3,4-dione
ADP + 2-hydroxy-3,4-dioxononyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyundecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoundecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1,2-dihydroxyundecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoundecyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
Escherichia coli W3110 / ATCC 27325
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: -
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
-
Substrates: i.e. AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
Escherichia coli W3110 / ATCC 27325
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: no phosphorylation of deoxy-AI-2-analogues by LsrK. Synthesis of substrate analogues and activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: no phosphorylation of deoxy-AI-2-analogues by LsrK. Synthesis of substrate analogues and activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also phosphorylates and activates C-1 alkyl analogues of autoinducer-2, AI-2
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2,3,4,5-tetrachloro-6-(2,3,4,5-tetrachloroanilino)benzoic acid
-
2-(1,3-benzothiazol-2-yl)-5-(5-methylfuran-2-yl)-3-oxopentanenitrile
-
2-(3-chloro-2-methylanilino)benzoic acid
-
2-(3-chloroanilino)benzoic acid
-
2-(4-chloroanilino)benzoic acid
-
2-anilino-4-chlorobenzoic acid
-
2-[2-chloro-5-(trifluoromethyl)anilino]-5-methoxybenzoic acid
-
7-dehydroxypryogallin-4-carboxylic acid
-
HPr
a phosphocarrier protein central to the sugar phosphotransferase system of Escherichia coli. HPr copurifies with LsrK. LsrK activity is inhibited when bound to HPr. HPr inhibited LsrK activity in a mixed manner of competitive and uncompetitive, not via competitive or uncompetitive inhibition alone. Phosphorylation of HPr reduces its inhibitory effect on LsrK
-
HPr H15A
the HPr H15A mutant displays almost no inhibition of the enzyme
-
HPr H15E
the HPr H15E mutant displays several times lower inhibition compared to the wild-type
-
rosolic acid
rosolic acid positively stabilizes His-LsrK in thermal shift at high concentrations, suggesting a low affnity binding interaction between the two, high target specificity
[(3-chloro-2-methylphenyl)amino]benzoic acid
-
additional information
structure-based virtual screening of LsrK kinase inhibitors to target quorum sensing, overview. LsrK 3D modeling and virtual screening of a locally available database. Identified LsrK inhibitors can be further optimized as antivirulence agents
-
additional information
poor inhibition by aurin tricarboxylic acid
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.058
(S)-4,5-dihydroxypentane-2,3-dione
recombinant enzyme, pH and temperature not specified in the publication
1.4
1,2-dihydroxyheptane-3,4-dione
-
pH and temperature not specified in the publication
1.1
1,2-dihydroxyhexane-3,4-dione
-
pH and temperature not specified in the publication
0.27
1,2-dihydroxyoctane-3,4-dione
-
pH and temperature not specified in the publication
1
4,5-dihydroxypentane-2,3-dione
-
pH and temperature not specified in the publication
additional information
additional information
-
the bi-substrate kinetic mechanism is rapid equilibrium ordered, Michaelis-Menten kinetics, overview
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
17
(S)-4,5-dihydroxypentane-2,3-dione
recombinant enzyme, pH and temperature not specified in the publication
5.8
1,2-dihydroxyheptane-3,4-dione
-
pH and temperature not specified in the publication
5.8
1,2-dihydroxyhexane-3,4-dione
-
pH and temperature not specified in the publication
1.5
1,2-dihydroxyoctane-3,4-dione
-
pH and temperature not specified in the publication
7.6
4,5-dihydroxypentane-2,3-dione
-
pH and temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
293.1
(S)-4,5-dihydroxypentane-2,3-dione
recombinant enzyme, pH and temperature not specified in the publication
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00008
HPr
inhibition of recombinant enzyme, pH and temperature not specified in the publication
-
0.0012
HPr H15E
inhibition of recombinant enzyme, pH and temperature not specified in the publication
-
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.025
1,2-dihydroxydecane-3,4-dione
Salmonella enterica
-
pH and temperature not specified in the publication
0.0053
1,2-dihydroxyheptane-3,4-dione
Salmonella enterica
-
pH and temperature not specified in the publication
0.05
1,2-dihydroxyhexane-3,4-dione
Salmonella enterica
-
above, pH and temperature not specified in the publication
0.005
1,2-dihydroxyoctane-3,4-dione
Salmonella enterica
-
pH and temperature not specified in the publication
0.003
7-deacetoxy-7-oxokhivorin
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.007
7-dehydroxypryogallin-4-carboxylic acid
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.004
acetyl isogambogic acid
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.007
Agaric acid
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.001
Aurin tricarboxylic acid
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.004
celastrol
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.007
fumarprotocetraric acid
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.01
harpagoside
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.009
protoporphyrin IX
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.001
rosolic acid
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.003
stictic acid
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.009
tetrachloroisophtalonitrile
Salmonella enterica subsp. enterica serovar Typhimurium
pH 7.4, 22°C, recombinant enzyme
0.011
2,3,4,5-tetrachloro-6-(2,3,4,5-tetrachloroanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.025 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.012
2,3,4,5-tetrachloro-6-(2,3,4,5-tetrachloroanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.1 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.032
2-(3-chloro-2-methylanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.025 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.07
2-(3-chloro-2-methylanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.1 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.023
2-(3-chloroanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.025 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.119
2-(3-chloroanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.1 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.036
2-(4-chloroanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.025 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.141
2-(4-chloroanilino)benzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.1 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.02
2-anilino-4-chlorobenzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.025 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.03
2-anilino-4-chlorobenzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.1 mM ATP, pH 7.4, 22°C, recombinant enzyme
0.031
2-[2-chloro-5-(trifluoromethyl)anilino]-5-methoxybenzoic acid
Salmonella enterica subsp. enterica serovar Typhimurium
at 0.025 mM ATP, pH 7.4, 22°C, recombinant enzyme
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
22
assay at room temperature
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
brenda
-
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
HPr, a phosphocarrier protein central to the sugar phosphotransferase system of Escherichia coli, copurifies with LsrK. LsrK activity is increased in the stationary phase
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
additional information
malfunction
-
in the DELTAlsrK deletion mutant, expression of the lsr-lacZ fusion is not induced by the presence of luxS or by exogenously supplied AI-2 in contrast to the wild-type. The enzyme activity and ATPase activity in the presence of culture fluids prepared from a luxS null strain is consistently lower than the background level
malfunction
-
no induction of the lsr promoter occurs in the lsrK mutant strain, since the lsr promoter is activated by phosphorylated autoinducer-2. Intracellular accumulation of phosphorylated autoinducer-2 is not observed in a lsrK mutant
malfunction
-
unlike the wild-type, the lsrK mutant does not activate transcription of the lsr operon in response to AI-2. The lsrK mutant fails to activate lsr expression because it cannot produce phospho-AI-2, which is the lsr operon inducer. Specifically, in an lsrK mutant, any AI-2 internalized by a secondary transporter does not get phosphorylated, and cannot be sequestered. In an lsrR/lsrK double mutant extracellular AI-2 accumulates to wild-type levels
malfunction
-
deletion of lsrR and lsrK does not affect growth or motility. Genomic deletion of gene lsrK. Genes lsrK and lsrR knockouts behave identically concerning the quantity and architecture of biofilms by affecting genes including flu and wza, small riboregulators respond to the QS regulators lsrR and lsrK
malfunction
inhibition of LsrK impairs the quorum-sensing cascade and consequently reduces bacterial pathogenicity
malfunction
inhibition of LsrK leads to quorum sensing inactivation and interfere with the pathogenesis
malfunction
-
inhibition of LsrK impairs the quorum-sensing cascade and consequently reduces bacterial pathogenicity
-
malfunction
-
inhibition of LsrK leads to quorum sensing inactivation and interfere with the pathogenesis
-
malfunction
-
in the DELTAlsrK deletion mutant, expression of the lsr-lacZ fusion is not induced by the presence of luxS or by exogenously supplied AI-2 in contrast to the wild-type. The enzyme activity and ATPase activity in the presence of culture fluids prepared from a luxS null strain is consistently lower than the background level
-
malfunction
-
inhibition of LsrK impairs the quorum-sensing cascade and consequently reduces bacterial pathogenicity
-
malfunction
-
inhibition of LsrK leads to quorum sensing inactivation and interfere with the pathogenesis
-
metabolism
-
model-based design, construction, and characterization of controller cells to modulate the external AI-2 environment, the cells are designed via the compartmentalization of different aspects of AI-2 processing: uptake (lsrACDB), phosphorylation (lsrK), and degradation (lsrFG), mathematical model overview
metabolism
-
Lsr-mediated transport and processing of autoinducer 2, AI-2, overview
metabolism
-
autoinducer 2, AI-2, is imported by the Lsr and is phosphorylated by the LsrK kinase, and AI-2-phosphate is the inducer of the lsr operon. LsrK is necessary for the phosphorylation of AI-2, AI-2 is not phosphorylated by phosphoenolpyruvate phosphotransferase system, which is involved in the AI-2 internalization. Lsr-mediated transport and processing of AI-2 in Escherichia coli, overview
metabolism
-
the enzyme is involved in quorum sensing, overview
metabolism
-
the enzyme is involved in quorum sensing, one type of bacterial cell-to-cell communication, which co-ordinates bacterial behaviors within a population. The communication is mediated by a plethora of diffusible signaling molecules, called autoinducers. AI-2 signaling is carried out by the Lsr proteins
metabolism
-
the regulatory network for the uptake of Escherichia coli autoinducer 2 (AI-2) is comprised of a transporter complex, LsrABCD, its repressor, LsrR, and the cognate signal kinase, LsrK. This network is an integral part of the AI-2 quorum-sensing system, extended model for AI-2 signaling, overview
metabolism
linkages between quorum sensing (QS) activity and sugar metabolism, model for LsrK/HPr interplay between QS and PTS, overview
metabolism
molecular communication among bacteria occurs via quorum sensing (QS). Modulating molecular communication among bacteria can alter phenotype, including attachment to epithelia, biofilm formation, and even toxin production. Intercepting and modulating communication networks provide a means to attenuate virulence without directly interacting with the bacteria of interest
metabolism
Escherichia coli W3110 / ATCC 27325
-
the enzyme is involved in quorum sensing, overview
-
metabolism
-
Lsr-mediated transport and processing of autoinducer 2, AI-2, overview
-
metabolism
-
the enzyme is involved in quorum sensing, overview
-
metabolism
-
the enzyme is involved in quorum sensing, overview
-
physiological function
-
the enzyme is responsible for the phosphorylation of autoinducer 2, and thereby attenuating cell-cell communication among bacterial populations. The bacterial signal molecule autoinducer-2 is secreted as a quorum sensing signal. The enzyme LsrK increases the net uptake rate by limiting secretion of AI-2 back into the extracellular environment. LsrK-mediated phosphorylation of AI-2 blocks export back to the extracellular milieu so that accumulated AI-2P binds the regulatory protein LsrR
physiological function
-
LsrK is required for the regulation of the lsr operon and the AI-2 uptake process. LsrK is a kinase that phosphorylates AI-2 upon entry into the cell, phosphorylation of AI-2 results in its sequestration in the cytoplasm
physiological function
-
enzyme LsrK activity, namely, AI-2 phosphorylation, is required to sequester the signal in the cytoplasm, independently of the mechanism of AI-2 internalization
physiological function
-
autoinducer-2, AI-2, is activated by the bacterial kinase LsrK and modulate AI-2 specific gene transcription through the transcriptional regulator LsrR. Activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview. In vivo, the critical step that makes AI-2 functional in derepressing the lsr operon is phosphorylation by the kinase LsrK. Active autinducer 2 and its analogues quench the quorum sensing response in multiple bacterial species simultaneously, overview
physiological function
-
when enzyme-treated autoinducer-2 is added ex vivo to Escherichia coli populations, the native quorum sensing response is significantly reduced, overview. The addition of enzyme LsrK and ATP to growing co-cultures of Escherichia coli and Salmonella typhimurium exhibits significantly reduced native cross-talk that ordinarily exists among and between species in an ecosystem
physiological function
-
enteric bacteria respond to the presence of competitor bacteria by sequestering and destroying autoinducer-2, through phosphorylation by the enzyme LsrK and cleavage of 4-hydroxy-2,3-pentanedione 5-phosphate producing 2-phosphoglycolic acid via enzyme LsrG, thereby eliminating the competitors' intercellular communication capabilities. The phosphorylated form of AI-2 is the intracellular signal responsible for lsr activation
physiological function
-
in enteric bacteria, the kinase LsrK catalyzes the phosphorylation of the C5-hydroxyl group in the linear form of 4,5-dihydroxypentane-2,3-dione, the precursor of the type II bacterial quorum sensing molecule (AI-2), the enzyme is required for phosphorylation is required for autoinducer-2 sequestration in the cytoplasm and subsequent derepression of AI-2 related genes necessary for quorum development and autoinducer-2 quorum sensing activation, catalytic mechanism, overview. Phosphorylated AI-2 (phospho-DPD) binds and inactivates the transcriptional repressor protein LsrR thereby inducing the expression of the lsr operon and potentially other LsrR-regulated, QS-related genes
physiological function
-
autoinducer-2, AI-2, is activated by the bacterial kinase LsrK and modulate AI-2 specific gene transcription through the transcriptional regulator LsrR. Activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview. In vivo, the critical step that makes AI-2 functional in derepressing the lsr operon is phosphorylation by the kinase LsrK. Active autinducer 2 and its analogues quench the quorum sensing response in multiple bacterial species simultaneously, overview
physiological function
-
autoinducer-2, AI-2, is activated by the bacterial kinase LsrK and modulate AI-2 specific gene transcription through the transcriptional regulator LsrR. Activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview. In vivo, the critical step that makes AI-2 functional in derepressing the lsr operon is phosphorylation by the kinase LsrK. Active autinducer 2 and its analogues quench the quorum sensing response in multiple bacterial species simultaneously, overview
physiological function
-
the enzyme is repsonsible for the regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli, overview. Enzyme LsrK and the Lsr transporter are required for AI-2 internalization and processing in Escherichia coli
physiological function
-
repressor LsrR and kinase LsrK directly regulate autoinducer 2, AI-2, uptake. Small riboregulators respond to the QS regulators lsrR and lsrK. 117 and 32 genes are induced and repressed, respectively, by lsrK
physiological function
-
genes lsrK and crp are required for optimal biofilm formation by Aggregatibacter actinomycetemcomitans. Another kinase is capable of phosphorylating AI-2 in Aggregatibacter actinomycetemcomitans in the absence of LsrK or enzyme LsrK is not essential for regulating the lsrACDBFG and lsrRK operons in AAggregatibacter actinomycetemcomitans
physiological function
LsrK is a kinase playing a key role in the processing of autoinducer-2 (AI-2), a quorum-sensing mediator in gut enteric bacteria
physiological function
quorum sensing (QS), a bacterial process that regulates population-scale behavior, is mediated by small signaling molecules, called autoinducers (AIs), that are secreted and perceived, modulating a collective phenotype. Because the autoinducer AI-2 is secreted by a wide variety of bacterial species, its perception cues bacterial behavior. This response is mediated by the lsr (LuxS-regulated) operon that includes the AI-2 transporter LsrACDB and the kinase LsrK. The AI-2-mediated QS system is partially regulated by substrate availability and cell metabolism. LuxS synthesizes AI-2 as a by-product of the activated methyl cycle, after which AI-2 accumulates extracellularly. AI-2 is imported by LsrACDB and phosphorylated by the kinase LsrK, sequestering it within the cell. LsrK is tightly bound to the PTS protein HPr, and HPr directly influences LsrK activity
physiological function
communication mediated by the quorum sensing (QS) requires bacterial autoinducer-2, AI-2 ((S)-4,5-dihydroxypentane-2,3-dione). AI-2 mediated QS activity is diminished by phosphorylation via the AI-2 processing kinase, LsrK
physiological function
LsrK is a kinase responsible for the phosphorylation of autoinducer-2, a signaling molecule involved in quorum sensing, the method of bacterial communication
physiological function
Escherichia coli W3110 / ATCC 27325
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autoinducer-2, AI-2, is activated by the bacterial kinase LsrK and modulate AI-2 specific gene transcription through the transcriptional regulator LsrR. Activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview. In vivo, the critical step that makes AI-2 functional in derepressing the lsr operon is phosphorylation by the kinase LsrK. Active autinducer 2 and its analogues quench the quorum sensing response in multiple bacterial species simultaneously, overview
-
physiological function
-
autoinducer-2, AI-2, is activated by the bacterial kinase LsrK and modulate AI-2 specific gene transcription through the transcriptional regulator LsrR. Activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview. In vivo, the critical step that makes AI-2 functional in derepressing the lsr operon is phosphorylation by the kinase LsrK. Active autinducer 2 and its analogues quench the quorum sensing response in multiple bacterial species simultaneously, overview
-
physiological function
-
enteric bacteria respond to the presence of competitor bacteria by sequestering and destroying autoinducer-2, through phosphorylation by the enzyme LsrK and cleavage of 4-hydroxy-2,3-pentanedione 5-phosphate producing 2-phosphoglycolic acid via enzyme LsrG, thereby eliminating the competitors' intercellular communication capabilities. The phosphorylated form of AI-2 is the intracellular signal responsible for lsr activation
-
physiological function
-
LsrK is a kinase playing a key role in the processing of autoinducer-2 (AI-2), a quorum-sensing mediator in gut enteric bacteria
-
physiological function
-
LsrK is a kinase responsible for the phosphorylation of autoinducer-2, a signaling molecule involved in quorum sensing, the method of bacterial communication
-
physiological function
-
LsrK is required for the regulation of the lsr operon and the AI-2 uptake process. LsrK is a kinase that phosphorylates AI-2 upon entry into the cell, phosphorylation of AI-2 results in its sequestration in the cytoplasm
-
physiological function
-
autoinducer-2, AI-2, is activated by the bacterial kinase LsrK and modulate AI-2 specific gene transcription through the transcriptional regulator LsrR. Activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview. In vivo, the critical step that makes AI-2 functional in derepressing the lsr operon is phosphorylation by the kinase LsrK. Active autinducer 2 and its analogues quench the quorum sensing response in multiple bacterial species simultaneously, overview
-
physiological function
-
autoinducer-2, AI-2, is activated by the bacterial kinase LsrK and modulate AI-2 specific gene transcription through the transcriptional regulator LsrR. Activities of phosphorylated autoinducer 2 analogues in quorum sensing reduction, overview. In vivo, the critical step that makes AI-2 functional in derepressing the lsr operon is phosphorylation by the kinase LsrK. Active autinducer 2 and its analogues quench the quorum sensing response in multiple bacterial species simultaneously, overview
-
physiological function
-
LsrK is a kinase playing a key role in the processing of autoinducer-2 (AI-2), a quorum-sensing mediator in gut enteric bacteria
-
physiological function
-
LsrK is a kinase responsible for the phosphorylation of autoinducer-2, a signaling molecule involved in quorum sensing, the method of bacterial communication
-
additional information
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LsrK-mediated degradation of autoinducer-2 attenuates the quorum sensing response among Salmonella typhimurium and Vibrio harveyi even though the autoinducer-2 signal transduction mechanisms and the phenotypic responses are species-specific
additional information
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analysis of structure-activity relationship, overview
additional information
three-dimensional homology structure modeling of LsrK, overview. LsrK kinase has two domains: N-terminal domain of residues 13-260 (domain I) and C-terminal domain of residues 271-468 (domain II). These two domains form a prominent cleft at their interface including both the ATP and the substrate binding sites. This putative binding site constituted both the ATP binding site and the substrate binding site. The substrate binding site is located deep in the cleft, whereas the ATP binding site is located near the entrance of the pocket. (S)-4,5-Dihydroxypentane-2,3-dione (DPD) docking results show that the C5 position of DPD, where the phosphorylation occurs, orients toward ATP by forming interactions with Thr21
additional information
-
three-dimensional homology structure modeling of LsrK, overview. LsrK kinase has two domains: N-terminal domain of residues 13-260 (domain I) and C-terminal domain of residues 271-468 (domain II). These two domains form a prominent cleft at their interface including both the ATP and the substrate binding sites. This putative binding site constituted both the ATP binding site and the substrate binding site. The substrate binding site is located deep in the cleft, whereas the ATP binding site is located near the entrance of the pocket. (S)-4,5-Dihydroxypentane-2,3-dione (DPD) docking results show that the C5 position of DPD, where the phosphorylation occurs, orients toward ATP by forming interactions with Thr21
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additional information
-
three-dimensional homology structure modeling of LsrK, overview. LsrK kinase has two domains: N-terminal domain of residues 13-260 (domain I) and C-terminal domain of residues 271-468 (domain II). These two domains form a prominent cleft at their interface including both the ATP and the substrate binding sites. This putative binding site constituted both the ATP binding site and the substrate binding site. The substrate binding site is located deep in the cleft, whereas the ATP binding site is located near the entrance of the pocket. (S)-4,5-Dihydroxypentane-2,3-dione (DPD) docking results show that the C5 position of DPD, where the phosphorylation occurs, orients toward ATP by forming interactions with Thr21
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Show AA Sequence and Transmembrane Helices (792 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
HisLsrK-HPr protein complex, crystal structure and gel filtration
additional information
additional information
LsrK kinase has two domains: N-terminal domain of residues 13-260 (domain I) and C-terminal domain of residues 271-468 (domain II). These two domains form a prominent cleft at their interface including both the ATP and the substrate binding sites
additional information
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LsrK kinase has two domains: N-terminal domain of residues 13-260 (domain I) and C-terminal domain of residues 271-468 (domain II). These two domains form a prominent cleft at their interface including both the ATP and the substrate binding sites
-
additional information
-
LsrK kinase has two domains: N-terminal domain of residues 13-260 (domain I) and C-terminal domain of residues 271-468 (domain II). These two domains form a prominent cleft at their interface including both the ATP and the substrate binding sites
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant LsrK/HPr complexes, i.e. selenomethionine-labeled His-tagged LsrK/HPr, His-tagged LsrK/HPr, His-tagged LsrK/HPr/ADP, and His-tagged LsrK/HPr/ATP, hanging drop vapor diffusion method, 15 mg/ml protein complex is mixed with reservoir buffer containing 15 to 20% v/v PEG 400, 0.1 M sodium phosphate-citrate, pH 4.2, and 0.2 M lithium sulfate, with or without addition of 5 mM MgCl2, and 5 mM ATP, or ADP, or ATPgammaS, X-ray diffraction structure determination and analysis at 2.7-3.2 A resolution
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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generation of chromosomal lsrK deletion (DELTAlsrK), or a chromosomal lsrRK double-deletion (DELTAlsrRK), the transcriptional activities of lsrR-P and lsrA-P do not differ significantly in the wild-type and lsrK-deficient backgrounds, while in the absence of LsrR, the transcriptional activity of both lsrR-P and lsrA-P is increased relative to that of the wild-type strain
additional information
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genomic deletion of gene lsrK. Genes lsrK and lsrR knockouts behave identically concerning the quantity and architecture of biofilms by affecting genes including flu and wza, small riboregulators respond to the QS regulators lsrR and lsrK, e.g. the sRNA cell division inhibitor DicF is induced by at least twofold in both lsrR and lsrK mutants. 117 and 32 genes are induced and repressed, respectively, by lsrK, transcriptosome analysis in enzyme mutants, overview
additional information
assembly of a capsule of biological polymers alginate and chitosan, with attached/encapsulated AI-2 processing kinase LsrK and provided substrate ATP, for the enzymatic alteration of AI-2 in culture fluids. The functionalized capsules, when introduced into populations of bacteria, alter the dynamics of the AI-2 bacterial communication, attenuating QS activated phenotypes. Method, overview
additional information
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construction of an in frame deletion of the lsrK gene, phenotype, overview
additional information
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construction of an in frame deletion of the lsrK gene, phenotype, overview
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain MET1158 by nickel affinity chromatography, and desalting gel filtration
recombinant His-tagged LsrK-Gln and LsrK-Tyr from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography and gel filtration
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recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, anion exchange chromatography, and gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene lsrK, encoded in the lsrKR operon where lsrK codes for an AI-2 kinase and lsrR encodes a repressor of the lsr operon, sequences important for the optimal transcription of lsrRK are located between -89 and -255 bp. LsrR regulates the expression of both lsrACDBFG and lsrRK operons. Transcription of the lsr locus is complex and may be driven by several promoters
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gene lsrK, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21
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gene lsrK, recombinant expression of N-terminally His-tagged enzyme as C-terminally glutamine-tagged LsrK-Gln and tyrosine-tagged LsrK-Tyr in Escherichia coli strain BL21(DE3)
gene lsrK, recombinant overexpresison of His-tagged enzyme in Escherichia coli strain MET1158
gene lsrK, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
cAMP-catabolite activator protein (CAP)-dependent activation of the lsr transcription. A cAMP-CAP-independent mechanism of lsr activation involves LsrR, the lsr operon repressor
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glycerol-3-phosphate inhibits lsr operon transcription activation by cAMP-catabolite activator protein, CAP. Dihydroxyacetone phosphate represses lsr transcription by a cAMP-CAP-independent mechanism involving LsrR, the lsr operon repressor, dihydroxyacetone phosphate causes repression by an LsrR-dependent and catabolite repression-independent route
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LsrR regulates the expression of both lsrACDBFG and lsrRK operons
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phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) binds specifically to the repressor of the lsr operon, LsrR, consistent with P-DPD being the inducer of the lsr operon
transcription of lsrK is not regulated by luxS or AI-2
phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) binds specifically to the repressor of the lsr operon, LsrR, consistent with P-DPD being the inducer of the lsr operon
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phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) binds specifically to the repressor of the lsr operon, LsrR, consistent with P-DPD being the inducer of the lsr operon
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transcription of lsrK is not regulated by luxS or AI-2
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transcription of lsrK is not regulated by luxS or AI-2
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug development
drug development
the enzyme is a target for inhibitor development to establish quorum-sensing inhibition, one of the most promising strategies to face the threat of bacterial resistance
drug development
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the enzyme is a target for inhibitor development to establish quorum-sensing inhibition, one of the most promising strategies to face the threat of bacterial resistance
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drug development
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the enzyme is a target for inhibitor development to establish quorum-sensing inhibition, one of the most promising strategies to face the threat of bacterial resistance
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pereira, C.; Santos, A.; Bejerano-Sagie, M.; Correia, P.; Marques, J.; Xavier, K.
Phosphoenolpyruvate phosphotransferase system regulates detection and processing of the quorum sensing signal autoinducer-2
Mol. Microbiol.
84
93-104
2012
Escherichia coli
brenda
Xavier, K.B.; Miller, S.T.; Lu, W.; Kim, J.H.; Rabinowitz, J.; Pelczer, I.; Semmelhack, M.F.; Bassler, B.L.
Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria
ACS Chem. Biol.
2
128-136
2007
Salmonella enterica
brenda
Roy, V.; Fernandes, R.; Tsao, C.Y.; Bentley, W.E.
Cross species quorum quenching using a native AI-2 processing enzyme
ACS Chem. Biol.
5
223-232
2010
Escherichia coli
brenda
Roy, V.; Smith, J.A.; Wang, J.; Stewart, J.E.; Bentley, W.E.; Sintim, H.O.
Synthetic analogs tailor native AI-2 signaling across bacterial species
J. Am. Chem. Soc.
132
11141-11150
2010
Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli, Vibrio harveyi, Escherichia coli W3110 / ATCC 27325, Vibrio harveyi BB170, Salmonella enterica subsp. enterica serovar Typhimurium MET708, Salmonella enterica subsp. enterica serovar Typhimurium MET715
brenda
Zhu, J.; Hixon, M.S.; Globisch, D.; Kaufmann, G.F.; Janda, K.D.
Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation
J. Am. Chem. Soc.
135
7827-7830
2013
Salmonella enterica
brenda
Xavier, K.B.; Bassler, B.L.
Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli
J. Bacteriol.
187
238-248
2005
Escherichia coli
brenda
Li, J.; Attila, C.; Wang, L.; Wood, T.K.; Valdes, J.J.; Bentley, W.E.
Quorum sensing in Escherichia coli is signaled by AI-2/LsrR: effects on small RNA and biofilm architecture
J. Bacteriol.
189
6011-6020
2007
Escherichia coli
brenda
Torres-Escobar, A.; Juarez-Rodriguez, M.D.; Lamont, R.J.; Demuth, D.R.
Transcriptional regulation of Aggregatibacter actinomycetemcomitans lsrACDBFG and lsrRK operons and their role in biofilm formation
J. Bacteriol.
195
56-65
2013
Aggregatibacter actinomycetemcomitans
brenda
Zargar, A.; Quan, D.N.; Emamian, M.; Tsao, C.Y.; Wu, H.C.; Virgile, C.R.; Bentley, W.E.
Rational design of controller cells to manipulate protein and phenotype expression
Metab. Eng.
30
61-68
2015
Escherichia coli
brenda
Taga, M.E.; Miller, S.T.; Bassler, B.L.
Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium
Mol. Microbiol.
50
1411-1427
2003
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium MET235
brenda
Ha, J.H.; Hauk, P.; Cho, K.; Eo, Y.; Ma, X.; Stephens, K.; Cha, S.; Jeong, M.; Suh, J.Y.; Sintim, H.O.; Bentley, W.E.; Ryu, K.S.
Evidence of link between quorum sensing and sugar metabolism in Escherichia coli revealed via cocrystal structures of LsrK and HPr
Sci. Adv.
4
eaar7063
2018
Escherichia coli (P77432)
brenda
Rhoads, M.K.; Hauk, P.; Terrell, J.; Tsao, C.Y.; Oh, H.; Raghavan, S.R.; Mansy, S.S.; Payne, G.F.; Bentley, W.E.
Incorporating LsrK AI-2 quorum quenching capability in a functionalized biopolymer capsule
Biotechnol. Bioeng.
115
278-289
2018
Escherichia coli (P77432)
brenda
Medarametla, P.; Gatta, V.; Kajander, T.; Laitinen, T.; Tammela, P.; Poso, A.
Structure-based virtual screening of LsrK kinase inhibitors to target quorum sensing
ChemMedChem
13
2400-2407
2018
Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZKQ6), Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 (Q8ZKQ6), Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 (Q8ZKQ6)
brenda
Gatta, V.; Ilina, P.; Porter, A.; McElroy, S.; Tammela, P.
Targeting quorum sensing high-throughput screening to identify novel LsrK inhibitors
Int. J. Mol. Sci.
20
3112
2019
Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZKQ6), Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 (Q8ZKQ6), Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 (Q8ZKQ6)
brenda
EXTERNAL LINKS (specific for EC-Number 2.7.1.189)
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