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Information on EC 2.7.1.177 - L-threonine kinase
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The expected taxonomic range for this enzyme is: Salmonella enterica
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EC NUMBER 
COMMENTARY 
2.7.1.177
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RECOMMENDED NAME
GeneOntology No.
L-threonine kinase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + L-threonine = ADP + O-phospho-L-threonine
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-
-
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ATP + L-threonine = ADP + O-phospho-L-threonine
steady-state ordered BiBI mechanism
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
ATP:L-threonine O3-phosphotransferase
The enzyme is involved in the de novo synthesis of adenosylcobalamin. It is specific for ATP and free L-threonine. In the bacterium Salmonella enterica the activity with CTP, GTP, or UTP is 6, 11, and 3% of the activity with ATP.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Streptomyces coelicolor
the protein CouR3, encoded by gene couR3, exhibits an ATPase activity similar to its close orthologue, the threonine kinase PduX, but it does not show a threonine kinase activity
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
involved in the biosynthesis of adenosylcobalamin
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + L-threonine
ADP + O-phospho-L-threonine
-
product indentified by 31P NMR spectroscopy
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?
additional information
?
-
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the activity with CTP, GTP, or UTP is 6, 11, 3% of the activity with ATP, respectively
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-
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additional information
?
-
the activity with CTP, GTP, or UTP is 6, 11, 3% of the activity with ATP, respectively
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0547
ATP
-
Michaelis-Menten kinetics, pH 7.4, temperature not specified in the publication
0.0547
ATP
-
wild-type, pH 7.4, temperature not specified in the publication
0.068
ATP
-
mutant D135N, pH 7.4, temperature not specified in the publication
0.069
ATP
-
calculated from double-reciprocal plots, pH 7.4, temperature not specified in the publication
0.074
ATP
-
mutant S255A, pH 7.4, temperature not specified in the publication
0.076
ATP
-
mutant S253A, pH 7.4, temperature not specified in the publication
0.087
ATP
-
mutant T134A, pH 7.4, temperature not specified in the publication
0.098
ATP
-
mutant T101A, pH 7.4, temperature not specified in the publication
0.117
ATP
-
mutant E24Q, pH 7.4, temperature not specified in the publication
0.141
ATP
-
mutant D103N, pH 7.4, temperature not specified in the publication
0.163
ATP
-
mutant D103A, pH 7.4, temperature not specified in the publication
1.264
ATP
-
mutant E132Q, pH 7.4, temperature not specified in the publication
1.374
ATP
-
mutant S99A, pH 7.4, temperature not specified in the publication
1.979
ATP
-
mutant E132A, pH 7.4, temperature not specified in the publication
0.127
L-threonine
-
calculated from double-reciprocal plots, pH 7.4, temperature not specified in the publication
0.142
L-threonine
-
mutant T134A, pH 7.4, temperature not specified in the publication
0.144
L-threonine
-
mutant E132Q, pH 7.4, temperature not specified in the publication
0.146
L-threonine
-
Michaelis-Menten kinetics, pH 7.4, temperature not specified in the publication
0.146
L-threonine
-
wild-type, pH 7.4, temperature not specified in the publication
0.176
L-threonine
-
mutant S99A, pH 7.4, temperature not specified in the publication
0.184
L-threonine
-
mutant D103N, pH 7.4, temperature not specified in the publication
0.198
L-threonine
-
mutant E132A, pH 7.4, temperature not specified in the publication
0.22
L-threonine
-
mutant T101A, pH 7.4, temperature not specified in the publication
0.258
L-threonine
-
mutant D103A, pH 7.4, temperature not specified in the publication
0.273
L-threonine
-
mutant D135N, pH 7.4, temperature not specified in the publication
0.464
L-threonine
-
mutant E24Q, pH 7.4, temperature not specified in the publication
3.708
L-threonine
-
mutant S255A, pH 7.4, temperature not specified in the publication
4.698
L-threonine
-
mutant S253A, pH 7.4, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0173
ATP
-
mutant D135N, pH 7.4, temperature not specified in the publication
0.0187
ATP
-
mutant E24Q, pH 7.4, temperature not specified in the publication
0.0205
ATP
-
mutant E132A, pH 7.4, temperature not specified in the publication
0.0227
ATP
-
mutant S255A, pH 7.4, temperature not specified in the publication
0.026
ATP
-
mutant S253A, pH 7.4, temperature not specified in the publication
0.0272
ATP
-
mutant E132Q, pH 7.4, temperature not specified in the publication
0.0313
ATP
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mutant D103A, pH 7.4, temperature not specified in the publication
0.0338
ATP
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mutant D103N, pH 7.4, temperature not specified in the publication; mutant S99A, pH 7.4, temperature not specified in the publication
0.0342
ATP
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mutant T134A, pH 7.4, temperature not specified in the publication
0.0355
ATP
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mutant T101A, pH 7.4, temperature not specified in the publication
0.408
ATP
-
wild-type, pH 7.4, temperature not specified in the publication
0.016
L-threonine
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mutant D135N, pH 7.4, temperature not specified in the publication
0.0188
L-threonine
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mutant E24Q, pH 7.4, temperature not specified in the publication
0.0193
L-threonine
-
mutant E132A, pH 7.4, temperature not specified in the publication
0.0238
L-threonine
-
mutant S255A, pH 7.4, temperature not specified in the publication
0.0258
L-threonine
-
mutant E132Q, pH 7.4, temperature not specified in the publication
0.0287
L-threonine
-
mutant S253A, pH 7.4, temperature not specified in the publication
0.0322
L-threonine
-
mutant D103A, pH 7.4, temperature not specified in the publication
0.0325
L-threonine
-
mutant S99A, pH 7.4, temperature not specified in the publication
0.0353
L-threonine
-
mutant T134A, pH 7.4, temperature not specified in the publication
0.0358
L-threonine
-
mutant D103N, pH 7.4, temperature not specified in the publication
0.037
L-threonine
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mutant T101A, pH 7.4, temperature not specified in the publication
0.0427
L-threonine
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wild-type, pH 7.4, temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.000104
ATP
-
mutant E132A, pH 7.4, temperature not specified in the publication
0.00022
ATP
-
mutant E132Q, pH 7.4, temperature not specified in the publication
0.000247
ATP
-
mutant S99A, pH 7.4, temperature not specified in the publication
0.00159
ATP
-
mutant E24Q, pH 7.4, temperature not specified in the publication
0.00193
ATP
-
mutant D103A, pH 7.4, temperature not specified in the publication
0.0024
ATP
-
mutant D103N, pH 7.4, temperature not specified in the publication
0.00257
ATP
-
mutant D135N, pH 7.4, temperature not specified in the publication
0.00308
ATP
-
mutant S255A, pH 7.4, temperature not specified in the publication
0.00342
ATP
-
mutant S253A, pH 7.4, temperature not specified in the publication
0.00363
ATP
-
mutant T101A, pH 7.4, temperature not specified in the publication
0.00393
ATP
-
mutant T134A, pH 7.4, temperature not specified in the publication
0.00755
ATP
-
wild-type, pH 7.4, temperature not specified in the publication
0.000061
L-threonine
-
mutant S253A, pH 7.4, temperature not specified in the publication
0.0000643
L-threonine
-
mutant S255A, pH 7.4, temperature not specified in the publication
0.000407
L-threonine
-
mutant E24Q, pH 7.4, temperature not specified in the publication
0.000587
L-threonine
-
mutant D135N, pH 7.4, temperature not specified in the publication
0.00098
L-threonine
-
mutant E132A, pH 7.4, temperature not specified in the publication
0.00125
L-threonine
-
mutant D103A, pH 7.4, temperature not specified in the publication
0.00168
L-threonine
-
mutant T101A, pH 7.4, temperature not specified in the publication
0.0018
L-threonine
-
mutant E132Q, pH 7.4, temperature not specified in the publication
0.00185
L-threonine
-
mutant S99A, pH 7.4, temperature not specified in the publication
0.00195
L-threonine
-
mutant D103N, pH 7.4, temperature not specified in the publication
0.00248
L-threonine
-
mutant T134A, pH 7.4, temperature not specified in the publication
0.00292
L-threonine
-
wild-type, pH 7.4, temperature not specified in the publication
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35000
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.177)
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