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Information on EC 2.7.1.151 - inositol-polyphosphate multikinase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FLT2

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IUBMB Comments
This enzyme also phosphorylates Ins(1,4,5)P3 to Ins(1,3,4,5)P4, Ins(1,3,4,5)P4 to Ins(1,3,4,5,6)P5, and Ins(1,3,4,5,6)P4 to Ins(PP)P4, isomer unknown. The enzyme from the plant Arabidopsis thaliana can also phosphorylate Ins(1,3,4,6)P4 and Ins(1,2,3,4,6)P5 at the D-5-position to produce 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate (InsP6), respectively . Yeast produce InsP6 from Ins(1,4,5)P3 by the actions of this enzyme and EC 2.7.1.158, inositol-pentakisphosphate 2-kinase .
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Arabidopsis thaliana
UNIPROT: Q9FLT2
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
5-kinase, arg82, ins(1,4,5)p3 3-kinase, inositol polyphosphate multikinase, itpk1, inositol 1,4,5-trisphosphate 3-kinase, argriii, inositol polyphosphate kinase, atipk2beta, ip3 3-kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
inositol polyphosphate 6-/3-kinase
-
IP3/IP4 6-/3-kinase
-
AtIpk2a
AtIpk2b
AtIpk2beta
-
-
inositol 1,4,5-trisphosphate 3-kinase
inositol polyphosphate 6-/3-kinase
inositol polyphosphate kinase
-
-
-
-
IP3 3-kinase
-
-
IP3/IP4 6-/3-kinase
-
Ipk2/Impk/IP3K
-
-
Ipk2beta/IP3K
-
-
phosphoinositol kinase
-
-
-
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho-group transfer
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol-1,4,5-trisphosphate 6-phosphotransferase
This enzyme also phosphorylates Ins(1,4,5)P3 to Ins(1,3,4,5)P4, Ins(1,3,4,5)P4 to Ins(1,3,4,5,6)P5, and Ins(1,3,4,5,6)P4 to Ins(PP)P4, isomer unknown. The enzyme from the plant Arabidopsis thaliana can also phosphorylate Ins(1,3,4,6)P4 and Ins(1,2,3,4,6)P5 at the D-5-position to produce 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate (InsP6), respectively [3]. Yeast produce InsP6 from Ins(1,4,5)P3 by the actions of this enzyme and EC 2.7.1.158, inositol-pentakisphosphate 2-kinase [4].
CAS REGISTRY NUMBER
COMMENTARY hide
9077-69-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol 1,2,3,4,6-pentakisphosphate
ADP + 1D-myo-inositol 1,2,3,4,5,6-hexakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate
ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
show the reaction diagram
-
-
-
?
2 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
2 ADP + 2 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
?
3 ATP + 2 1D-myo-inositol 1,4,5-trisphosphate
3 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,2,3,4,6-pentakisphosphate
ADP + 1D-myo-inositol 1,2,3,4,5,6-hexakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,2,3,4,6-pentakisphosphate
ADP + inositol hexakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
ADP + ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
r
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol 1,4,5-trisphosphate
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate
ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
2 ADP + 2 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
?
3 ATP + 2 1D-myo-inositol 1,4,5-trisphosphate
3 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,2,3,4,6-pentakisphosphate
ADP + inositol hexakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
ADP + ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
r
ATP + 1D-myo-inositol 1,4,5-trisphosphate
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate
ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
show the reaction diagram
-
-
-
-
ir
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
abscisic acid
-
In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level decreased.
NaCl
-
In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level decreases in response to salt
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
indole-3-acetic acid
The expression of AtIpk2b is enhanced in roots after treatment with 1 microM. Expression of AtIpk2b become stronger when the concentration of exogenous indole-3-acetic acid is increased to 10 microM. By treatment with 40 microM, AtIpk2b expression increases after 0.5 h of treatment, whereas the expression of AtIpk2a remains virtually unaffected. These results indicate that AtIpk2b is an IAA-inducible gene, while AtIpk2a is not.
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016 - 0.568
1D-myo-inositol 1,3,4,5-tetrakisphosphate
0.032 - 0.254
1D-myo-inositol 1,4,5,6-tetrakisphosphate
0.015 - 0.162
1D-myo-inositol 1,4,5-trisphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.8 - 106
1D-myo-inositol 1,3,4,5-tetrakisphosphate
0.97 - 79.1
1D-myo-inositol 1,4,5,6-tetrakisphosphate
29.7 - 37.8
1D-myo-inositol 1,4,5-trisphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
isozyme Ipk2beta
Manually annotated by BRENDA team
-
isozyme Ipk2beta
Manually annotated by BRENDA team
-
Ten-day-old Arabidopsis seedlings grown on MS medium are used for different treatments. RNA is extracted and reverse transcription of RNA is carried out using M-MLV RTase Synthesis Kit.
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IPMKB_ARATH
300
0
33487
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
calculated and confirmed by Western Blot analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
domain structure analysis, the enzyme contains an IP3-binding domain and an ATP/Mg2+ binding domain, but no F-actin-binding or CaM-binding domain, and no nucleus localization signal sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
isoform inositol phosphate multikinase alpha, to 2.9 A resolution. The structural core of Arabidopsis thaliana IPMK is homologous to those of Saccharamyces cerevisiae IPMK and Homo sapiens IP3K
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D100A
isozyme IPK2beta, site-directed mutagenesis, inactive mutant
D98A
isozyme IPK2alpha, site-directed mutagenesis, inactive mutant
K117W
mutant exhibits nearly normal 6-kinase function but harbors significantly reduced 3-kinase activity. The mutant complements conditional nutritional growth defects observed in ipmk null yeast and suppresses lethality observed in ipmk null flies
K117W/K121W
mutant exhibits nearly normal 6-kinase function but harbors significantly reduced 3-kinase activity. The mutant complements conditional nutritional growth defects observed in ipmk null yeast and suppresses lethality observed in ipmk null flies
K121W
although no inositol tetrakisphosphate is present with wild-type IPMK, levels of inositol tetrakisphosphate and inositol hexakisphosphate are equivalent in mutant K121W
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
1 min, inactivation, purified recombinant isozyme
100
1 min, inactivation, purified recombinant isozyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GST-fusion isozymes from Escherichia coli by glutathione affinity chromatography
recombinant GST-fusion isozymes from Escherichia coli by glutathione affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Lycopersicon esculentum cv. PKM
isozyme Ipk2beta, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain
Expression of AtIpk2b in yeast mutant arg82D, quantitative real-time PCR analysis of AtIpk2b expression in Arabidopsis. AtIpk2b cDNA containing the complete open reading frame is inserted via XbaI-SalI sites into the binary vector pBinAR-HPT. Resulting construct is introduced into Agrobacterium tumefaciens (strain LBA4404). Tobacco leaf disks are infected with the transformed Agrobacteria.
-
isozyme Ipk2alpha, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain
To create an AtIpk2b overexpression construct, the AtIpk2b cDNA is cut from plasmid pmIP3K with XbaI-XhoI and inserted via XbaI-SalI sites into pBinAR-HPT vector behind the cauliflower mosaic virus 35S promoter. The resulting plasmid is first introduced into Agrobacterium tumefaciens by electroporation and then transformed Arabidopsis ecotype C24 by vacuum infiltration. Transgenic plants are selected on MS medium containing 20 mg/L hygromycin. Eleven AtIpk2b overexpression plants, named OX-2, OX-3, OX-5, OX-8, OX-9, OX-15, OX-25, OX-26, OX-33, OX-35, and OX-64, are identified. Compared to the wild type, branching of axillary shoots is more pronounced in the overexpression lines.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
transgenic tomato plants show increased tolerance to drought, cold and oxidative stress
medicine
-
putative treatment of cancer
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xia, H.J.; Yang, G.
Inositol 1,4,5-trisphosphate 3-kinases: functions and regulations
Cell Res.
15
83-91
2005
Arabidopsis thaliana, Homo sapiens, Saccharomyces cerevisiae
Manually annotated by BRENDA team
Stevenson-Paulik, J.; Odom, A.R.; York, J.D.
Molecular and biochemical characterization of two plant inositol polyphosphate 6-/3-/5-kinases
J. Biol. Chem.
277
42711-42718
2002
Arabidopsis thaliana (Q9FLT2), Arabidopsis thaliana (Q9LY23), Arabidopsis thaliana
Manually annotated by BRENDA team
Resnick, A.C.; Saiardi, A.
Inositol polyphosphate multikinase: metabolic architect of nuclear inositides
Front. Biosci.
13
856-866
2008
Arabidopsis thaliana, Saccharomyces cerevisiae, Drosophila melanogaster, Homo sapiens, Rattus norvegicus, Solanum tuberosum
Manually annotated by BRENDA team
Yang, L.; Tang, R.; Zhu, J.; Liu, H.; Mueller-Roeber, B.; Xia, H.; Zhang, H.
Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2beta, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana
Plant Mol. Biol.
66
329-343
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Zhang, Z.B.; Yang, G.; Arana, F.; Chen, Z.; Li, Y.; Xia, H.J.
Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2beta) is involved in axillary shoot branching via auxin signaling
Plant Physiol.
144
942-951
2007
Arabidopsis thaliana (Q9LY23)
Manually annotated by BRENDA team
Zhang, Y.; Liu, H.; Li, B.; Zhang, J.T.; Li, Y.; Zhang, H.
Generation of selectable marker-free transgenic tomato resistant to drought, cold and oxidative stress using the Cre/loxP DNA excision system
Transgenic Res.
18
607-619
2009
Arabidopsis thaliana (Q9FLT2), Arabidopsis thaliana
Manually annotated by BRENDA team
Endo-Streeter, S.; Tsui, M.K.; Odom, A.R.; Block, J.; York, J.D.
Structural studies and protein engineering of inositol phosphate multikinase
J. Biol. Chem.
287
35360-35369
2012
Arabidopsis thaliana (Q9LY23), Arabidopsis thaliana
Manually annotated by BRENDA team