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Information on EC 2.7.1.151 - inositol-polyphosphate multikinase and Organism(s) Rattus norvegicus and UniProt Accession Q99NI4

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IUBMB Comments
This enzyme also phosphorylates Ins(1,4,5)P3 to Ins(1,3,4,5)P4, Ins(1,3,4,5)P4 to Ins(1,3,4,5,6)P5, and Ins(1,3,4,5,6)P4 to Ins(PP)P4, isomer unknown. The enzyme from the plant Arabidopsis thaliana can also phosphorylate Ins(1,3,4,6)P4 and Ins(1,2,3,4,6)P5 at the D-5-position to produce 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate (InsP6), respectively . Yeast produce InsP6 from Ins(1,4,5)P3 by the actions of this enzyme and EC 2.7.1.158, inositol-pentakisphosphate 2-kinase .
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Rattus norvegicus
UNIPROT: Q99NI4
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The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
5-kinase, arg82, ins(1,4,5)p3 3-kinase, inositol polyphosphate multikinase, itpk1, inositol 1,4,5-trisphosphate 3-kinase, argriii, inositol polyphosphate kinase, atipk2beta, ip3 3-kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
inositol polyphosphate kinase
-
-
-
-
inositol polyphosphate multikinase
-
-
Ins(1,4,5)P3 3-kinase
-
-
phosphoinositol kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho-group transfer
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol-1,4,5-trisphosphate 6-phosphotransferase
This enzyme also phosphorylates Ins(1,4,5)P3 to Ins(1,3,4,5)P4, Ins(1,3,4,5)P4 to Ins(1,3,4,5,6)P5, and Ins(1,3,4,5,6)P4 to Ins(PP)P4, isomer unknown. The enzyme from the plant Arabidopsis thaliana can also phosphorylate Ins(1,3,4,6)P4 and Ins(1,2,3,4,6)P5 at the D-5-position to produce 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate (InsP6), respectively [3]. Yeast produce InsP6 from Ins(1,4,5)P3 by the actions of this enzyme and EC 2.7.1.158, inositol-pentakisphosphate 2-kinase [4].
CAS REGISTRY NUMBER
COMMENTARY hide
9077-69-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
inositol (1,3,4,5)-tetrakisphosphate + ATP
inositol pentakisphosphate + ADP
show the reaction diagram
-
-
?
inositol (1,4,5)-triphosphate + ATP
inositol (1,3,4,5)-tetrakisphosphate + ADP
show the reaction diagram
-
-
?
inositol (4,5)-bisphosphate + ATP
inositol (1,4,5)-trisphosphate + ADP
show the reaction diagram
-
-
?
inositol pentakisphosphate + ATP
diphosphoinositol tetrakisphosphate + ADP
show the reaction diagram
-
-
?
2 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
2 ADP + 2 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
in rats and humans
-
-
?
2 ATP + 1D-myo-inositol 4,5-bisphosphate
2 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
-
-
?
3 ATP + 2 1D-myo-inositol 1,4,5-trisphosphate
3 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
in rats and humans
-
-
?
ATP + 1D-myo-inositol 4,5-bisphosphate
ADP + 1D-myo-inositol 3,4,5-trisphosphate
show the reaction diagram
ATP + phosphatidylinositol 4,5-bisphosphate
ADP + phosphatidylinositol 3,4,5-trisphosphate
show the reaction diagram
-
in rats
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
2 ADP + 2 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
in rats and humans
-
-
?
2 ATP + 1D-myo-inositol 4,5-bisphosphate
2 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
-
-
?
3 ATP + 2 1D-myo-inositol 1,4,5-trisphosphate
3 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
in rats and humans
-
-
?
ATP + 1D-myo-inositol 4,5-bisphosphate
ADP + 1D-myo-inositol 3,4,5-trisphosphate
show the reaction diagram
-
-
-
-
?
ATP + phosphatidylinositol 4,5-bisphosphate
ADP + phosphatidylinositol 3,4,5-trisphosphate
show the reaction diagram
-
in rats
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no inhibition by wortmannin and LY294002
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
1D-myo-inositol 4,5-bisphosphate
-
pH 7.4, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5
-
partially purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest expression in cerebellum, lowest in hippocampus, intermediate in cerebral cortex
Manually annotated by BRENDA team
most highly expressed
Manually annotated by BRENDA team
-
primary hippocampal and corical cultures
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IPMK_RAT
396
0
44410
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44400
x * 44400, deduced from nucleotide sequence
44000
-
x * 44000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 44400, deduced from nucleotide sequence
?
-
x * 44000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme partially by nuclear fraction preparation
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged enzyme, expression in Escherichia coli
expression of HA-tagged enzyme in human HEK293 and in COS-7 cells
-
IPMK knockout mice demonstrates the critical roles of IPMK in embryogenesis and central nevous system development. Deletion of IPMK in mice results in early lethality.
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
putative treatment of cancer
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Saiardi, A.; Nagata, E.; Luo, H.R.; Sawa, A.; Luo, X.; Snowman, A.M.; Snyder, S.H.
Mammalian inositol polyphosphate multikinase synthesizes inositol 1,4,5-trisphosphate and an inositol pyrophosphate
Proc. Natl. Acad. Sci. USA
98
2306-2311
2001
Rattus norvegicus (Q99NI4)
Manually annotated by BRENDA team
Resnick, A.C.; Snowman, A.M.; Kang, B.N.; Hurt, K.J.; Snyder, S.H.; Saiardi, A.
Inositol polyphosphate multikinase is a nuclear PI3-kinase with transcriptional regulatory activity
Proc. Natl. Acad. Sci. USA
102
12783-12788
2005
Rattus norvegicus, Saccharomyces cerevisiae
Manually annotated by BRENDA team
Resnick, A.C.; Saiardi, A.
Inositol polyphosphate multikinase: metabolic architect of nuclear inositides
Front. Biosci.
13
856-866
2008
Arabidopsis thaliana, Saccharomyces cerevisiae, Drosophila melanogaster, Homo sapiens, Rattus norvegicus, Solanum tuberosum
Manually annotated by BRENDA team