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Information on EC 2.7.1.146 - ADP-specific phosphofructokinase and Organism(s) Thermococcus zilligii and UniProt Accession Q9HH12

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IUBMB Comments
ADP can be replaced by GDP, ATP and GTP, to a limited extent. Divalent cations are necessary for activity, with Mg2+ followed by Co2+ being the most effective.
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This record set is specific for:
Thermococcus zilligii
UNIPROT: Q9HH12
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The taxonomic range for the selected organisms is: Thermococcus zilligii
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
adp-dependent phosphofructokinase, tlpfk, tk0376, adp-dependent 6-phosphofructokinase, adp-dependent pfk, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ADP dependent phosphofructokinase
-
-
-
-
ADP-6-phosphofructokinase
-
-
-
-
ADP-dependent phosphofructokinase
-
-
-
-
ADP-Pfk
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ADP:D-fructose-6-phosphate 1-phosphotransferase
ADP can be replaced by GDP, ATP and GTP, to a limited extent. Divalent cations are necessary for activity, with Mg2+ followed by Co2+ being the most effective.
CAS REGISTRY NUMBER
COMMENTARY hide
237739-62-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-fructose 6-phosphate + ADP
D-fructose 1,6-bisphosphate + AMP
show the reaction diagram
-
-
-
r
D-fructose 6-phosphate + ADP
D-fructose 1,6-bisphosphate + AMP
show the reaction diagram
-
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-fructose 6-phosphate + ADP
D-fructose 1,6-bisphosphate + AMP
show the reaction diagram
-
-
-
r
D-fructose 6-phosphate + ADP
D-fructose 1,6-bisphosphate + AMP
show the reaction diagram
-
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
20% of activity
Mg2+
-
required for activity
Mn2+
-
65% of activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
K6PF_THEZI
461
0
52758
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
200000
gel filtration
52700
calculation from sequence
53900
4 * 53900, SDS-PAGE
42200
-
x * 42200, SDS-PAGE
66000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 53900, SDS-PAGE
?
-
x * 42200, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ronimus, R.S.; Koning, J.; Morgan, H.W.
Purification and characterization of an ADP-dependent phosphofructokinase from Thermococcus zilligii
Extremophiles
3
121-129
1999
Thermococcus zilligii
Manually annotated by BRENDA team
Ronimus, R.S.; de Heus, E.; Morgan, H.W.
Sequencing, expression, characterization and phylogeny of the ADP-dependent phosphofructokinase from the hyperthermophilic, euryarchaeal Thermococcus zilligii
Biochim. Biophys. Acta
1517
384-391
2001
Thermococcus zilligii (Q9HH12), Thermococcus zilligii
Manually annotated by BRENDA team