Any feedback?
Information on EC 2.7.1.138 - ceramide kinase
for references in articles please use BRENDA:EC2.7.1.138Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.1.138 ceramide kinaseSpecify your search results
Word Map
- 2.7.1.138
- ceramide-1-phosphate
- sphingolipids
- 1-phosphate
- sphingosine-1-phosphate
- ceramidase
-
pleckstrin
-
cerkl
- medicine
-
ceramide-induced
- analysis
- diagnostics
The enzyme appears in viruses and cellular organisms
Synonyms
ceramide kinase, hcerk, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acylsphingosine kinase
-
-
-
-
ceramide kinase
CERK
hCERK
kinase, acylsphingosine (phosphorylating)
-
-
-
-
additional information
-
the enzyme belongs to the subfamily of diacylglyceride kinases DAGKs
CERK
-
-
CERK
-
CERK
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + ceramide = ADP + ceramide 1-phosphate
-
-
-
-
ATP + ceramide = ADP + ceramide 1-phosphate
Leu10 in the pleckstrin homology domain of the enzyme is crucial for catalytic activity
-
ATP + ceramide = ADP + ceramide 1-phosphate
the secondary hydroxy group is not required for substrate recognition, preference for the D-erythro isomer, the enzyme is specific for th phosphorylation of the 1-hydroxy group
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
Phosphorylation
Phosphorylation
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
ATP:ceramide 1-phosphotransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
123175-68-8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-OH-C24:0 phytoceramide + ATP
alpha-OH-C24:0 phytoceramide 1-phosphate + ADP
-
-
-
-
?
alpha-OH-D-erythro-C6:0 ceramide + ATP
alpha-OH-D-erythro-C6:0 ceramide 1-phosphate + ADP
-
-
-
-
?
ATP + 3-O-methyl C16:0 ceramide
ADP + 3-O-methyl C16:0 ceramide 1-phosphate
-
very low activity
-
-
?
ATP + C8-dihydroceramide
ADP + C8-dihydroceramide 1-phosphate
-
high activity
-
-
?
ATP + D-erythro-C6:0 ceramide
ADP + D-erythro-C6:0 ceramide 1-phosphate
-
very low activity
-
-
?
ATP + D-erythro-N-hexanoyl-sphinganine
ADP + D-erythro-N-hexanoyl-sphinganine 1-phosphate
-
-
-
?
ATP + D-erythro-N-hexanoyl-sphingenine
ADP + D-erythro-N-hexanoyl-sphingenine 1-phosphate
-
-
-
?
ATP + D-eythro-C10:0 ceramide
ADP + D-eythro-C10:0 ceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C12:0 ceramide
ADP + D-eythro-C12:0 ceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C14:0 ceramide
ADP + D-eythro-C14:0 ceramide 1-phosphate
-
best substrate
-
-
?
ATP + D-eythro-C16:0 (R)-alpha-hydroxyceramide
ADP + D-eythro-C16:0 (R)-alpha-hydroxyceramide 1-phosphate
-
low activity
-
-
?
ATP + D-eythro-C16:0 (S)-alpha-hydroxyceramide
ADP + D-eythro-C16:0 (S)-alpha-hydroxyceramide 1-phosphate
-
very low activity
-
-
?
ATP + D-eythro-C16:0 dehydroceramide
ADP + D-eythro-C16:0 dehydroceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C16:0 dihydroceramide
ADP + D-eythro-C16:0 dihydroceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C16:0 urea ceramide
ADP + D-eythro-C16:0 urea ceramide 1-phosphate
-
low activity, no activity with L-e-C16:0 urea ceramide
-
-
?
ATP + D-eythro-C16:0-cis ceramide
ADP + D-eythro-C16:0-cis ceramide 1-phosphate
-
low activity
-
-
?
ATP + D-eythro-C18:0 phytoceramide
ADP + D-eythro-C18:0 phytoceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C20:0 ceramide
ADP + D-eythro-C20:0 ceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C24:0 ceramide
ADP + D-eythro-C24:0 ceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C24:1 ceramide
ADP + D-eythro-C24:1 ceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C8:0 ceramide
ADP + D-eythro-C8:0 ceramide 1-phosphate
-
low activity
-
-
?
ATP + hexadecanoylceramide
ADP + hexadecanoylceramide 1-phosphate
C8 acyl chain, slightly higher activity than with natural ceramide
-
?
ATP + hexanoylceramide
ADP + hexanoylceramide 1-phosphate
C6 acyl chain, similar activity than with natural ceramide, very low activity with C2-ceramide and C6-dihydroceramide, no activity with C2-dihydroceramide
-
?
ATP + N-acetyl-D-erythro-sphingenine
ADP + N-acetyl-D-erythro-sphingenine 1-phosphate
-
-
-
?
ATP + N-acetyl-sphingenine
ADP + N-acetyl-sphingenine 1-phosphate
-
-
-
?
ATP + N-hexanoyl-1-O-hexadecyl-2-deoxy-2-amino-sn-glycerol
ADP + ?
-
-
-
?
ATP + N-hexanoyl-D-erythro-sphingenine
ADP + N-hexanoyl-D-erythro-sphingenine 1-phosphate
best substrate
-
-
?
ATP + N-hexanoyl-sphingenine
ADP + N-hexanoyl-sphingenine 1-phosphate
-
-
-
?
ATP + octanoylceramide
ADP + octanoylceramide 1-phosphate
C8 acyl chain, higher activity than with natural ceramide
-
?
ATP + octanoyldihydroceramide
ADP + octanoyldihydroceramide 1-phosphate
C8 acyl chain, slightly higher activity than with natural ceramide
-
?
D-erythro-C12:0 ceramide + ATP
D-erythro-C12:0 ceramide 1-phosphate + ADP
-
-
-
-
?
D-erythro-C14:0 ceramide + ATP
D-erythro-C14:0 ceramide 1-phosphate + ADP
-
-
-
-
?
D-erythro-C16:0 ceramide + ATP
D-erythro-C16:0 ceramide 1-phosphate + ADP
-
used for kinetic analysis of ceramide kinase activity
-
-
?
D-erythro-C2:0 ceramide + ATP
D-erythro-C2:0 ceramide 1-phosphate + ADP
-
-
-
-
?
D-erythro-C2:0 dihydroceramide + ATP
D-erythro-C2:0 dihydroceramide 1-phosphate + ADP
-
-
-
-
?
D-erythro-C6:0 ceramide + ATP
D-erythro-C6:0 ceramide 1-phosphate + ADP
-
-
-
-
?
D-erythro-C6:0 dihydroceramide + ATP
D-erythro-C6:0 dihydroceramide 1-phosphate + ADP
-
-
-
-
?
D-erythro-N-hexanoyl-sphingenine + ATP
D-erythro-N-hexanoyl-sphingenine 1-phosphate + ADP
-
D-erythro isomer of C6-ceramide, the specific CERK activity in cells exposed for 16-18 h to 0.005 mM C6-ceramide dropped to 18.7%
-
-
?
D-threo-C6-ceramide + ATP
D-threo-C6-ceramide 1-phosphate + ADP
-
no or only weakly decrease of the specific CERK activity
-
-
?
GTP + ceramide
GDP + ceramide 1-phosphate
-
26% of the activity with ATP at 1 mM
-
?
L-erythro-C6-ceramide + ATP
L-erythro-C6-ceramide 1-phosphate + ADP
-
no or only weakly decrease of the specific CERK activity
-
-
?
L-threo-C6-ceramide + ATP
L-threo-C6-ceramide 1-phosphate + ADP
-
no or only weakly decrease of the specific CERK activity
-
-
?
N-((4-(4,4-difluoro-5-(2-thienyl)-4-bora-3alpha,4alpha-diaza-s-indacene-3-yl)phenoxy)acetyl)sphingosine + ATP
N-((4-(4,4-difluoro-5- (2-thienyl)-4- bora-3a,4a-diaza-s-indacene-3-yl)phenoxy)acetyl)sphingosine 1-phosphate + ADP
-
TRB-ceramide
-
-
?
N-(12-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)dodecanoyl)-D-erythro-sphingosine + ATP
N-(12-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)dodecanoyl)-D-erythro-sphingosine 1-phosphate + ADP
-
C12-NBD ceramide
-
-
?
N-(5-(5,7-dimethyl-BODIPY)-L-pentanoyl)-D-erythrosphingosine + ATP
N-(5-(5,7-dimethyl-BODIPY)-L-pentanoyl)-D-erythrosphingosine 1-phosphate + ADP
-
DMB-C5 ceramide
-
-
?
N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine + ATP
N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine 1-phosphate + ADP
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythro-sphingosine + ATP
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythro-sphingosine 1-phosphate + ADP
NBD-ceramide
NBD-ceramide 1-phosphate
-
?
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythrosphingosine + ATP
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythrosphingosine 1-phosphate + ADP
N-(8-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)octanoyl)-D-erythro-sphingosine + ATP
N-(8-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)octanoyl)-D-erythro-sphingosine 1-phosphate + ADP
C8-ceramide
-
-
?
N-hexanoyl-sphinganine + ATP
N-hexanoyl-sphinganine 1-phosphate + ADP
-
C6-dihydroceramide
-
-
?
N-octyl-sphingenine + ATP
N-octyl-sphingenine 1-phosphate + ADP
-
C8-ceramine, no or only weakly decrease of the specific CERK activity
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
ceramide is an important regulator of photoreceptor viability
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
-
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
-
ceramide 1-phosphate is a lipid signalling molecule involved in regulation of many physiological processes related to cell proliferation, apoptosis, and inflammation, most of them in concert with sphingosine 1-phosphate, ceramide 1-phosphate might act via extracellular cell surface receptors or intracellularly as second messenger
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
-
synthesis of the sphingolipid signaling molecule
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
no activity sphingosine, D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine and phyto-sphingosine
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
no activity with 1,2-diacylglycerol
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
in vitro substrate delivery of short chain ceramides requires bovine serum albumin, and a mixed micellar form consisting of octylglucoside and cardiolipin for long chain ceramides, choice of delivery system is important for in vitro activity
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
stereospecific ceramide substrate recognition, the C4-5 trans double bond is important for substrate recognition, a minimum of 12C-acyl chain is required for activity
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
the enzyme is specific for the cramide backbone, acylation of amino group in position 2 is required
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
the pleckstrin homology domain is essential for activity, binding of liposomes
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
-
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
other name n-acyl-sphingosine, no activity with CTP, CDPcholine and UDPglucose
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
ceramide phosphorylation may be associated with neurotransmitter release
-
?
ATP + D-eythro-C16:0 ceramide
ADP + D-eythro-C16:0 ceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C16:0 ceramide
ADP + D-eythro-C16:0 ceramide 1-phosphate
-
high activity
-
-
?
ATP + D-eythro-C18:0 ceramide
ADP + D-eythro-C18:0 ceramide 1-phosphate
-
-
-
-
?
ATP + D-eythro-C18:0 ceramide
ADP + D-eythro-C18:0 ceramide 1-phosphate
-
high activity
-
-
?
ATP + N-hexadecanoyl-sphingenine
ADP + N-hexadecanoyl-sphingenine 1-phosphate
-
-
-
?
ATP + N-hexadecanoyl-sphingenine
ADP + N-hexadecanoyl-sphingenine 1-phosphate
very low activity
-
-
?
C2-ceramide + ATP
C2-ceramide 1-phosphate + ADP
C2-Cer is a potent inducer of cell death in CerK-expressing cells
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
CERK and its product Cer-1-P are implicated in signaling processes, Cer-1-P stimulates DNA synthesis
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
-
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
ceramide is a key mediator of cellular apoptosis
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
ceramide kinase is a critical mediator of eicosanoid synthesis, and its product, ceramide 1-phosphate, is required for the production of prostaglandins in response to several inflammatory agonists, CERK requires ceramide actively transported to the trans-Golgi network via ceramide transport protein CERT
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
ceramide kinase is important for cellular proliferation and survival, activation of cytosolic phospholipase A2, mast cell degranulation, and phagocytosis
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
CERK and C1P are playing significant biological roles in cell-signaling cascades
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
CerK has important roles in leukocyte functions, including the role in degranulation of mast cells and the phagocytosis of polymorphonuclear leukocytes
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
CERK plays a role in cell signalling
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
increased ceramide 1-phosphate during phagocytosis enhances phagocytosis and phagolysosome formation in COS-1 cells expressing hCERK
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
involvement in inflammatory processes and cell growth
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
key enzyme for controlling ceramide levels
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
-
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
ceramide 1-phosphate is a sphingolipid that participates in cell signaling, but ceramide kinase is not the only source of ceramide 1-phosphate
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
ceramide kinase is not the only source of ceramide 1-phosphate, ceramide kinase is important for neutrophil homeostasis, ceramide kinase is important for the defense against Streptococcus pneumoniae infections
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
CerK and C1P are involved in many cell functions, including membrane fusion, phagocytosis and degranulation in mast cells
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
CerK is not necessary for survival but might participate in neuronal functions involving emotional behavior
-
-
?
ceramide + ATP
ceramide 1-phosphate + ADP
-
CerK is involved in the regulation of mast cells
-
-
?
N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine + ATP
N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine 1-phosphate + ADP
C6-ceramide
-
-
?
N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine + ATP
N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine 1-phosphate + ADP
-
C6-NBD ceramide
-
-
?
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythrosphingosine + ATP
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythrosphingosine 1-phosphate + ADP
-
-
-
-
?
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythrosphingosine + ATP
N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythrosphingosine 1-phosphate + ADP
-
NBD-Cer, NBD-C6 ceramide
-
-
?
additional information
?
-
-
the enzyme does not play a role in neutrophil priming, the enzyme regulates the activity and translocalization of cytosolic phospholipase isozyme A2alpha, overview, the enzyme is involved in prostaglandin biosynthesis, i.e. intracellular arachidonic acid production, which is blocked by enzyme gene silencing
-
-
?
additional information
?
-
-
the enzyme is a critical mediator of eicosanoid synthesis
-
-
?
additional information
?
-
-
no activity with GTP, no activity with sphingosine and sphinganine, and with diacylglycerol
-
-
?
additional information
?
-
-
substrate specificity and stereochemistry, overview, methylation of the primary hydroxyl group results in loss of activity, the enzyme can use both ceramides with long saturated and long unsaturated fatty acyl chains
-
-
?
additional information
?
-
2-amino-1-hexadecanol, 1-O-hexadecyl-2-deoxy-2-amino-sn-glycerol, N-tetradecanoyl-2(R)-pyrrolidine-methanol, N-tetradecanoyl-(R)-serine, N-tetradecanoyl-3-hydroxypyrrolidin, N-tetradecanoyl-4-amino-1-butanol, N-tetradecanoyl-3-amino-1-propanol, N-tetradecanoyl-(S)-serine, N-tetradecanoyl-2-benzylserine, N-tetradecanoyl3-phenylserine, N-tetradecanoyl-3-amino-tyrosine, N-tetradecanoyl-1-amino-2-naphthol-4-sulfonic acid, N-tetradecanoyl-3-hydroxy-anthranilic acid, N-tetradecanoyl-4-aminophenol, N-tetradecanoyl-diethanolamine, N-tetradecanoyl-ethanolamine, and N-tetradecanoyl-(S)-phenylalaninol are no substrates
-
-
?
additional information
?
-
-
2-amino-1-hexadecanol, 1-O-hexadecyl-2-deoxy-2-amino-sn-glycerol, N-tetradecanoyl-2(R)-pyrrolidine-methanol, N-tetradecanoyl-(R)-serine, N-tetradecanoyl-3-hydroxypyrrolidin, N-tetradecanoyl-4-amino-1-butanol, N-tetradecanoyl-3-amino-1-propanol, N-tetradecanoyl-(S)-serine, N-tetradecanoyl-2-benzylserine, N-tetradecanoyl3-phenylserine, N-tetradecanoyl-3-amino-tyrosine, N-tetradecanoyl-1-amino-2-naphthol-4-sulfonic acid, N-tetradecanoyl-3-hydroxy-anthranilic acid, N-tetradecanoyl-4-aminophenol, N-tetradecanoyl-diethanolamine, N-tetradecanoyl-ethanolamine, and N-tetradecanoyl-(S)-phenylalaninol are no substrates
-
-
?
additional information
?
-
-
does not bind to lysophosphatidic acid, lysophosphocholine, sphingosine 1-phosphate, phosphatidylethanolamine, phosphatidylcholine, phosphatidic acid, or phosphatidylserine
-
-
?
additional information
?
-
activity of CerK in cells is measured by the formation of NBD-C1P from a substrate NBD-ceramide
-
-
-
additional information
?
-
-
the enzyme is a mediator of calcium-dependent degranulation in mast cells
-
-
?
additional information
?
-
-
the enzyme forms intracellular complexes with calmodulin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + N-hexadecanoyl-sphingenine
ADP + N-hexadecanoyl-sphingenine 1-phosphate
-
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
ceramide is an important regulator of photoreceptor viability
-
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
-
ceramide 1-phosphate is a lipid signalling molecule involved in regulation of many physiological processes related to cell proliferation, apoptosis, and inflammation, most of them in concert with sphingosine 1-phosphate, ceramide 1-phosphate might act via extracellular cell surface receptors or intracellularly as second messenger
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
-
synthesis of the sphingolipid signaling molecule
-
?
ATP + ceramide
ADP + ceramide 1-phosphate
-
-
-
-
?