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Information on EC 2.7.1.137 - phosphatidylinositol 3-kinase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P35169

for references in articles please use BRENDA:EC2.7.1.137
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EC Tree
IUBMB Comments
One mammalian isoform is known.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P35169
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Synonyms
pi3k, phosphatidylinositol 3-kinase, pi3 kinase, vps34, phosphoinositide-3-kinase, phosphatidylinositol 3 kinase, ptdins 3-kinase, pik3c3, phosphatidylinositide 3-kinase, hvps34, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
class III phosphatidylinositol 3-kinase
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class III phosphoinositide 3-kinase
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kinase (phosphorylating), phosphatidylinositol 3-
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kinase, phosphatidylinositol 3- (phosphorylating)
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lipid kinase Vps34
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p85/p110 phosphoinositide 3-kinase
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phosphatidylinositol 3'-kinase
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-
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phosphatidylinositol 3-kinase
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-
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phosphoinositide 3'-kinase
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phosphoinositide 3-kinase
PI 3-kinase
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-
-
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PI3-K-related protein MEC1
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PI3K
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-
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PtdIns 3'-kinase
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-
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vacuolar protein sorting 34
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VPS34
additional information
-
the enzyme belongs to the PI3-K family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase
One mammalian isoform is known.
CAS REGISTRY NUMBER
COMMENTARY hide
115926-52-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
show the reaction diagram
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
show the reaction diagram
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
show the reaction diagram
ATP + phosphatidylinositol-4,5-bisphosphate
ADP + phosphatidylinositol-3,4,5-trisphosphate
show the reaction diagram
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-
-
-
?
ATP + phosphatidylinositol-4-phosphate
ADP + phosphatidylinositol-3,4-bisphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
show the reaction diagram
ATP + phosphatidylinositol-4,5-bisphosphate
ADP + phosphatidylinositol-3,4,5-trisphosphate
show the reaction diagram
-
-
-
-
?
ATP + phosphatidylinositol-4-phosphate
ADP + phosphatidylinositol-3,4-bisphosphate
show the reaction diagram
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-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
vacuolar protein sorting 34 (Vps34) is a member of the phosphoinositide 3-kinase (PI3K) family of lipid kinases. Vps34 and its functions are highly conserved from yeast to mammals. The domain organization of Vps34 and Vps15 are highly conserved between yeast and humans
malfunction
the deletion of Vps34, Vps15 or Vps30 affects both autophagy and vacuolar protein sorting. The deletion of Vps34 or Vps15 results in a reduced mRNA production from G + C-rich coding sequences (CDS). Vps34 and Vps15 can enhance the efficiency of transcription elongation based on their physical proximity to nuclear pores and transcribed chromatin
metabolism
physiological function
additional information
domain organization of yeast Vps30, Atg14 and Vps38, structures of Complex I and II, crystal structure analysis of yeast Vps34 Complex II, overview
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D2243E
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site-directed mutagenesis, no functional complementation of a yeast enzyme-knockout mutant strain
D749E
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site-directed mutagenesis, no functional complementation of a yeast enzyme-knockout mutant strain
I670A
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site-directed mutagenesis, functional complementation of a yeast enzyme-knockout mutant strain
I670G
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site-directed mutagenesis, no functional complementation of a yeast enzyme-knockout mutant strain
L2129A
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site-directed mutagenesis, functional complementation of a yeast enzyme-knockout mutant strain
L2129G
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site-directed mutagenesis, functional complementation of a yeast enzyme-knockout mutant strain
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of wild-type VPS34 and PI3-K-related protein MEC1 in a enzyme-knockout mutant strain, functional complementation analysis, overview
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Huang, M.E.; Manus, V.; Chuat, J.C.; Galibert, F.
Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI
Yeast
12
869-875
1996
Saccharomyces cerevisiae (P35169)
Manually annotated by BRENDA team
Cafferkey, R.; Young, P.R.; McLaughlin, M.M.; Bergsma, D.J.; Koltin, Y.; Sathe, G.M.; Faucette, L.; Eng, W.K.; Johnson, R.K.; Livi, G.P.
Dominant missense mutations in a novel yeast protein related to mammalian phosphatidylinositol 3-kinase and VPS34 abrogate rapamycin cytotoxicity
Mol. Cell. Biol.
13
6012-6023
1993
Saccharomyces cerevisiae (P35169), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Helliwell, S.B.; Wagner, P.; Kunz, J.; Deuter-Reinhard, M.; Henriquez, R.; Hall, M.N.
TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast
Mol. Biol. Cell
5
105-118
1994
Saccharomyces cerevisiae (P35169)
Manually annotated by BRENDA team
Herman, P.K.; Emr, S.D.
Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae
Mol. Cell. Biol.
10
6742-6754
1990
Saccharomyces cerevisiae (P22543), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kunz, J.; Henriquez, R.; Schneider, U.; Deuter-Reinhard, M.; Movva, N.R.; Hall, M.N.
Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression
Cell
73
585-596
1993
Saccharomyces cerevisiae (P32600), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Schu, P.V.; Takegawa, K.; Fry, M.J.; Stack, J.H.; Waterfield, M.D.; Emr, S.D.
Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting
Science
260
88-91
1993
Saccharomyces cerevisiae (P22543), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Alaimo, P.J.; Knight, Z.A.; Shokat, K.M.
Targeting the gatekeeper residue in phosphoinositide 3-kinases
Bioorg. Med. Chem.
13
2825-2836
2005
Saccharomyces cerevisiae, Homo sapiens
Manually annotated by BRENDA team
Backer, J.M.
The intricate regulation and complex functions of the Class III phosphoinositide 3-kinase Vps34
Biochem. J.
473
2251-2271
2016
Saccharomyces cerevisiae (P22543), Mus musculus (Q6PF93), Homo sapiens (Q8NEB9)
Manually annotated by BRENDA team
Reidick, C.; Boutouja, F.; Platta, H.W.
The class III phosphatidylinositol 3-kinase Vps34 in Saccharomyces cerevisiae
Biol. Chem.
398
677-685
2017
Saccharomyces cerevisiae (P22543), Saccharomyces cerevisiae
Manually annotated by BRENDA team