Any feedback?
Information on EC 2.7.1.137 - phosphatidylinositol 3-kinase and Organism(s) Bos taurus and UniProt Accession P32871
for references in articles please use BRENDA:EC2.7.1.137Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.1.137 phosphatidylinositol 3-kinaseIUBMB Comments
One mammalian isoform is known.
Specify your search results
Word Map
- 2.7.1.137
- wortmannin
- mapks
- mtor
- rapamycin
- erk
- endothelial
- pten
-
signal-regulated
- pkc
- metastasis
- bcl-2
- sirna
- caspase-3
- agonist
- glycogen
- leukemia
- actin
- pik3ca
- phospholipase
-
insulin-like
-
mitogen
- cyclins
- integrins
- tumorigenesis
- irs-1
-
tensin
- tnf
-
neuroprotective
- c-jun
- pi3-kinase
-
dominant-negative
- adipocytes
- nf-kappab
-
anti-apoptotic
-
insulin-stimulated
- gtpases
-
insulin-induced
- akt1
-
transwell
-
platelet-derived
- rac1
- kinase-3
- igf-i
- sh2
- substrate-1
-
pleckstrin
- grb2
-
egf-induced
-
everolimus
-
tyrosine-phosphorylated
- drug development
- medicine
- pharmacology
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
pi3k, phosphatidylinositol 3-kinase, pi3 kinase, vps34, phosphoinositide-3-kinase, phosphatidylinositol 3 kinase, ptdins 3-kinase, pik3c3, phosphatidylinositide 3-kinase, hvps34, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kinase (phosphorylating), phosphatidylinositol 3-
-
-
-
-
kinase, phosphatidylinositol 3- (phosphorylating)
-
-
-
-
p85/p110 phosphoinositide 3-kinase
-
-
-
-
phosphatidylinositol 3'-kinase
-
-
-
-
phosphatidylinositol 3-kinase
-
-
-
-
phosphoinositide 3'-kinase
-
-
-
-
phosphoinositide 3-kinase
-
-
-
-
PI 3-kinase
-
-
-
-
PI3K
-
-
-
-
PtdIns 3'-kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase
One mammalian isoform is known.
CAS REGISTRY NUMBER
COMMENTARY
115926-52-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
?
ATP + 1-O-octadecyl-2-O-methyl-rac-3-glycerophospho-myo-inositol
ADP + ?
-
-
-
-
?
ATP + phosphatidylinositol 4,5-bisphosphate
ADP + phosphatidylinositol 3,4,5-trisphosphate
-
weak activity
-
-
?
ATP + phosphatidylinositol 4-phosphate
ADP + phosphatidylinositol 3,4-bisphosphate
-
weak activity
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
-
-
-
?
additional information
?
-
-
enzyme plays an important role in the signalling of cell growth
-
-
?
additional information
?
-
-
enzyme might contribute to the antiproliferative activity of the antitumor ether lipid analogs
-
-
?
additional information
?
-
-
PI 3-kinase activity is a necessary step in the regulation of bone resorption
-
-
?
additional information
?
-
-
enzyme plays an important role in the signaling of cell growth
-
-
?
additional information
?
-
-
receptor linked enzyme may generate a second-messenger signal
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme plays an important role in the signalling of cell growth
-
-
?
additional information
?
-
-
enzyme might contribute to the antiproliferative activity of the antitumor ether lipid analogs
-
-
?
additional information
?
-
-
PI 3-kinase activity is a necessary step in the regulation of bone resorption
-
-
?
additional information
?
-
-
enzyme plays an important role in the signaling of cell growth
-
-
?
additional information
?
-
-
receptor linked enzyme may generate a second-messenger signal
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
CaCl2 stimulates enzyme forms PI3KI and PI3KII in absence of MnCl2. Half-maximal activation of PI3KI at 0.1 mM Ca2+, maximal activity at 1 mM, in presence of 10 mM MgCl2 further 2fold activation by 2 mM Ca2+. For PI3KII maximal activity is obtained in presence of 2 mM Ca2+, 1.6fold higher than that obtained in presence of 10 mM MgCl2
Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+/-)-2-[hydroxy[tetrahydro-2-(octadecyloxy)methylfuran-2-yl]methoxyl]phosphinyloxy-N,N,N-trimethylethaniminium hydroxide
-
-
phosphatidylcholine
-
strong inhibition of enzyme form PI3KII, weak inhibition of enzyme form PI3KI
isoquercetin
-
strong inhibition of PI 3-kinase I and II, noncompetitive, apparent Ki value: 4 mM for PI 3-kinase I and 2.5 mM for PI 3-kinase II
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
phosphatidic acid
-
enhances activity more markedly for PI 3-kinase II than for PI 3-kinase I
additional information
-
insulin and IGF-I treatment increases phosphatidylinositol 3-kinase activity 4.5fold in aPY20 immunoprecipitation from whole cell lysates
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
activation of PI3K signaling is required for the inhibitory effect of Axl on mineral deposition
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PK3CA_BOVIN
1068
0
124328
Swiss-Prot
Secretory Pathway (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
110000
85000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
additional information
additional information
-
two types of PI 3-kinase a monomer form, PI 3-kinase I, and a heterodimeric form, PI 3-kinase II
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
phosphoprotein
-
putative PI 3-kinase 85000 Da subunit is phosphorylated by pp60vscr
phosphoprotein
-
purified enzyme complex is highly phosphorylated on both subunits, dephosphorylation generates a deactivated complex
phosphoprotein
-
highly phosphorylated on both p85a and p110 subunits, and dephosphorylation generates a deactivated complex, indicating that phosphorylation is an important covalent modification of the complex and may modulate PtdIns 3-kinase activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
p110 expressed in insect cells possesses Pl3-kinase activity and associates with p85 alpha into an active p85 alpha-p110 complex that binds the activated colony-stimulating factor 1 receptor. p110 expressed in COS-1 cells is catalytically active only when complexed with p85 alpha
expression of the p85 subunit and a related p85beta protein by using baculovirus expression system
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shibasaki, F.; Homma, Y.; Takenawa, T.
Two types of phosphatidylinositol 3-kinase from bovine thymus. Monomer and heterodimer form
J. Biol. Chem.
266
8108-8114
1991
Bos taurus
Morgan, S.J.; Smith, A.D.; Parker, P.J.
Purification and characterization of bovine brain type I phosphatidylinositol kinase
Eur. J. Biochem.
191
761-767
1990
Bos taurus
Fry, M.J.; Panayotou, G.; Dhand, R.; Ruiz-Larrea, F.; Gout, I.; Nguyen, O.; Courtneidge, S.A.; Waterfield, M.D.
Purification and characterization of a phosphatidylinositol 3-kinase complex from bovine brain by using phosphopeptide affinity columns
Biochem. J.
288
383-393
1992
Bos taurus
-
Shibasaki, F.; Fukui, Y.; Takenawa, T.
Different properties of monomer and heterodimer forms of phosphatidylinositol 3-kinases
Biochem. J.
289
227-231
1993
Bos taurus
-
Gout, I.; Dhand, R.; Panayotou, G.; Fry, M.J.; Hiles, I.; Otsu, M.; Waterfield, M.D.
Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system
Biochem. J.
288
395-405
1992
Bos taurus
-
Ruiz-Larrea, F.; Vicendo, P.; Yaish, P.; End, P.; Panayotou, G.; Fry, M.J.; Morgan, S.J.; Thompson, A.; Parker, P.J.; Waterfield, M.D.
Characterization of the bovine brain cytosolic phosphatidylinositol 3-kinase complex
Biochem. J.
290
609-616
1993
Bos taurus
-
Hiles, I.D.; Otsu, M.; Volinia, S.; Fry, M.J.; Gout, I.; Dhand, R.; Panayotou, G.; Ruiz-Larrea, F.; Thompson, A.; Totty, N.F.; Hsuan, J.J.; Courtneidge, S.A.; Parker, P.J.; Waterfield, M.D.
Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit
Cell
70
419-429
1992
Bos taurus, Bos taurus (P32871)
Berggren, M.I.; Gallegos, A.; Dressler, L.A.; Modest, E.J.; Powis, G.
Inhibition of the signalling enzyme phosphatidylinositol-3-kinase by antitumor ether lipid analogs
Cancer Res.
53
4297-4302
1993
Bos taurus, Mus musculus
Chakravorty, A.; Joslyn, M.I.; Davis, J.S.
Characterization of insulin and insulin-like growth factor-I actions in the bovine luteal cell: Regulation of receptor tyrosine kinase activity, phosphatidylinositol 3-kinase, and deoxyribonucleic acid synthesis
Endocrinology
133
1331-1340
1993
Bos taurus
End, P.; Gout, I.; Fry, M.J.; Panayotou, G.; Dhand, R.; Yonezawa, K.; Kasuga, M.; Waterfield, M.D.
A biosensor approach to probe the structure and function of the p85a subunit of the phosphatidylinositol 3-kinase complex
J. Biol. Chem.
268
10066-10075
1993
Bos taurus
Sato, M.; Bryant, H.U.; Dodge, J.A.; Davis, H.; Matter, W.F.; Vlahos, C.J.
Effects of wortmannin analogs on bone in vitro and in vivo
J. Pharmacol. Exp. Ther.
277
543-550
1996
Bos taurus, Gallus gallus, Rattus norvegicus
EXTERNAL LINKS (specific for EC-Number 2.7.1.137)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
