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ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
ATP + erythromycin
ADP + erythromycin 2'-O-phosphate
ATP + josamycin
ADP + josamycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + kitasamycin
?
-
52% activity with respect to oleandomycin for strain CU1, 61% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + kitasamycin
ADP + kitasamycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + neomycin
ADP + ?
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
ATP + rokitamycin
ADP + rokitamycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + roxithromycin
ADP + roxithromycin 2'-O-phosphate
ATP + spectinomycin
?
-
5% activity with respect to oleandomycin
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
ATP + telithromycin
ADP + telithromycin 2'-O-phosphate
ATP + triacetyloleandomycin
?
-
100% activity with respect to oleandomycin
-
-
?
ATP + triacetyloleandomycin + H2O
ADP + triacetyloleandomycin 2'-O-phosphate + acetate
-
as active as oleandomycin
-
-
?
ATP + troleandomycin
?
-
82% activity with respect to oleandomycin for strain CU1, 105% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + troleandomycin + H2O
ADP + troleandomycin 2'-O-phosphate + acetate
-
14-membered ring macrolide
-
-
?
ATP + tylosin
ADP + tylosin 2'-O-phosphate
ATP + tylosin
ADP + tylosin 2'-phosphate
additional information
?
-
ATP + azithromycin

ADP + azithromycin 2'-O-phosphate
-
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to azithromycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
-
15% of the activity with oleandomycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to azithromycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
-
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to azithromycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + clarithromycin

?
-
46-55% activity with respect to oleandomycin for strain CU1, 17% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + clarithromycin
?
-
77% activity with respect to oleandomycin
-
-
?
ATP + clarithromycin

ADP + clarithromycin 2'-O-phosphate
-
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
-
73% of the activity with oleandomycin
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
-
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + erythromycin

ADP + anhydroerythromycin-2'-O-phosphate
-
-
-
?
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
-
87% activity with respect to oleandomycin
-
-
?
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
-
81% activity with respect to oleandomycin for strain CU1, 24% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
-
88% activity with respect to oleandomycin
-
-
?
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
-
-
-
-
?
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
-
-
-
-
?
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
-
-
-
?
ATP + erythromycin
ADP + anhydroerythromycin-2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + erythromycin

ADP + erythromycin 2'-O-phosphate
-
-
-
-
ATP + erythromycin
ADP + erythromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + erythromycin
ADP + erythromycin 2'-O-phosphate
erythromycin resistance analyzed in recombinant Escherichia coli harbouring plasmid pRSB105
-
-
?
ATP + erythromycin
ADP + erythromycin 2'-O-phosphate
-
88% of the activity with oleandomycin
-
-
?
ATP + josamycin

?
-
1% activity with respect to oleandomycin
-
-
?
ATP + josamycin
?
-
75-95% activity with respect to oleandomycin for strain CU1, 96% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + josamycin
?
-
2% activity with respect to oleandomycin
-
-
?
ATP + midecamycin

?
-
3% activity with respect to oleandomycin
-
-
?
ATP + midecamycin
?
-
2% activity with respect to oleandomycin
-
-
?
ATP + oleandomycin

ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + roxithromycin

?
-
42% activity with respect to oleandomycin for strain CU1, about 2% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + roxithromycin
?
-
44% activity with respect to oleandomycin
-
-
?
ATP + roxithromycin

ADP + roxithromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + roxithromycin
ADP + roxithromycin 2'-O-phosphate
-
44% of the activity with oleandomycin
-
-
?
ATP + roxithromycin
ADP + roxithromycin 2'-O-phosphate
-
-
-
?
ATP + roxithromycin
ADP + roxithromycin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + spiramycin

ADP + spiramycin 2'-O-phosphate
-
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
57-75% activity with respect to oleandomycin for strain CU1, 26% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
2% activity with respect to oleandomycin
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
-
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
-
-
-
?
ATP + telithromycin

ADP + telithromycin 2'-O-phosphate
-
-
-
?
ATP + telithromycin
ADP + telithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to telithromycin
-
-
?
ATP + telithromycin
ADP + telithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to telithromycin
-
-
?
ATP + telithromycin
ADP + telithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to telithromycin
-
-
?
ATP + tylosin

ADP + tylosin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + tylosin
ADP + tylosin 2'-O-phosphate
-
-
-
?
ATP + tylosin
ADP + tylosin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + tylosin

ADP + tylosin 2'-phosphate
-
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
60% activity with respect to oleandomycin for strain CU1, 4% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
2% activity with respect to oleandomycin
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
-
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
-
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
-
-
?
additional information

?
-
-
substrate specificities of wild-type and mutant enzymes, structure-function considerations
-
-
-
additional information
?
-
under physiological conditions, enzyme exclusively uses ATP, no substrate: GTP
-
-
-
additional information
?
-
-
substrate specificities of wild-type and mutant enzymes, structure-function considerations
-
-
-
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0.0007
-
purified recombinant mutant H205A, substrate oleandomycin
0.04
-
purified recombinant wild-type enzyme, substrate roxithromycin or rokitamycin
0.05
-
purified recombinant wild-type enzyme, substrate josamycin or tylosin
0.052
-
purified recombinant mutant H198A, substrate oleandomycin
0.056
-
purified recombinant mutant H205N, substrate oleandomycin
0.06
-
purified recombinant wild-type enzyme, substrate troleandomycin, clarithromycin, azithromycin, or erythromycin
0.1
-
purified recombinant wild-type enzyme, substrate oleandomycin
0.16
-
purified recombinant wild-type enzyme, substrate kitasamycin
0.21
-
purified recombinant wild-type enzyme, substrate spiramycin
additional information

-
substrate specificities of the mutant enzymes
additional information
genetic map of the resistance plasmid pRSB105, functional antibiotic resistance regions of plasmid pRSB105 characterized, plasmid pRSB105 harbors the macrolide resistance gene mph, encoding a macrolide 2'-phosphotransferase, transcript analysis by RT-PCR, erythromycin resistance analyzed by determining values for minimum inhibitory concentration, mph gene shown to contribute to erythromycin resistance
additional information
accessory region on plasmid pRSB111 contains a new macrolide resistance operon, mph(E) gene encodes a macrolide phosphotransferase required for high-level macrolide resistance, resistance analyzed by determining values for minimum inhibitory concentration
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O'Hara, K.; Kanda,T.; Kono, M.
Structure of a phosphorylated derivative of oleandomycin, obtained by reaction of oleandomycin with an extract of an erythromycin-resistant strain of Escherichia coli
J. Antibiot.
41
823-827
1988
Escherichia coli (Q47396)
brenda
Marshall, V.P.; Coats, J.H.; Baczynskyj, L.; Cialdella, J.I.; Liggett, W.F.; McGee, J.E.
Purification of macrolide 2'-phosphotransferase from Streptomyces coelicolor Muller
J. Ind. Microbiol.
6
295-298
1990
Streptomyces coelicolor, Streptomyces coelicolor UC 5240
-
brenda
O'Hara, K.; Kanda,T.; Ohmiya, K.; Ebisu, T.; Kono, M.
Purification and characterization of macrolide 2'-phosphotransferase from a strain of Escherichia coli that is highly resistant to erythromycin
Antimicrob. Agents Chemother.
33
1354-1357
1989
Escherichia coli, Escherichia coli (Q47396)
brenda
Wiley, P.F.; Baczynskyj, L.; Dolak, L.A.; Cialdella, J.I.; Marshall, V.P.
Enzymatic phosphorylation of macrolide antibiotics
J. Antibiot.
40
195-201
1987
Streptomyces coelicolor, Streptomyces coelicolor UC 5240
brenda
Marshall, V.P.; Cialdella, J.I.; Baczynskyj, L.; Liggett, W.F.; Johnson, R.A.
Microbial O-phosphorylation of macrolide antibiotics
J. Antibiot.
62
132-134
1989
Streptomyces coelicolor, Streptomyces coelicolor UC 5240
-
brenda
Taniguchi, K.; Nakamura, A.; Tsurubuchi, K.; O'Hara, K.; Sawai, T.
Identification of Escherichia coli clinical isolates producing macrolide 2'-phosphotransferase by a highly sensitive detection method
FEMS Microbiol. Lett.
167
191-195
1998
Escherichia coli, Escherichia coli (Q47396)
brenda
Noguchi, N.; Katayama, J.
Expression in Pseudomonas aeruginosa of an erythromycin-resistance determinant that encodes the mphA gene for macrolide 2'-phosphotransferase I from Escherichia coli
Biol. Pharm. Bull.
21
191-193
1998
Escherichia coli, Escherichia coli (Q47396), Pseudomonas aeruginosa
brenda
Taniguchi, K.; Nakamura, A.; Tsurubuchi, K.; Ishii, A.; O'Hara, K.; Sawai, T.
Appearance in Japan of highly macrolide-resistant Escherichia coli producing macrolide 2'-phosphotransferase II
Microbios
97
137-144
1999
Escherichia coli
brenda
Taniguchi, K.; Nakamura, A.; Tsurubuchi, K.; Ishii, A.; O'Hara, K.; Sawai, T.
Identification of functional amino acids in the macrolide 2'-phosphotransferase II
Antimicrob. Agents Chemother.
43
2063-2065
1999
Escherichia coli
brenda
Noguchi, N.; Takada, K.; Katayama, J.; Emura, A.; Sasatsu, M.
Regulation of transcription of the mph(A) gene for macrolide 2'-phosphotransferase I in Escherichia coli: characterization of the regulatory gene mphR(A)
J. Bacteriol.
182
5052-5058
2000
Escherichia coli, Escherichia coli (Q47396)
brenda
Nakamura, A.; Miyakozawa, I.; Nakazawa, K.; O'Hara, K.; Sawai, T.
Detection and characterization of macrolide 2'-phosphotransferase from a Pseudomonas aeruginosa clinical isolate
Antimicrob. Agents Chemother.
44
3241-3242
2000
Pseudomonas aeruginosa
brenda
Taniguchi, K.; Nakamura, A.; Tsurubuchi, K.; O'Hara, K.; Sawai, T.
The role of histidine residues conserved in the putative ATP-binding region of macrolide 2'-phosphotransferase II
FEMS Microbiol. Lett.
232
123-126
2004
Escherichia coli, Escherichia coli CU1
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Chesneau, O.; Tsvetkova, K.; Courvalin, P.
Resistance phenotypes conferred by macrolide phosphotransferases
FEMS Microbiol. Lett.
269
317-322
2007
Escherichia coli (Q47396), Salmonella enterica subsp. enterica serovar Typhimurium (Q5QJG2), uncultured bacterium (A0AEF2), uncultured bacterium (Q5W3B4)
brenda
Szczepanowski, R.; Krahn, I.; Bohn, N.; Puehler, A.; Schlueter, A.
Novel macrolide resistance module carried by the IncP-1beta resistance plasmid pRSB111, isolated from a wastewater treatment plant
Antimicrob. Agents Chemother.
51
673-678
2007
uncultured bacterium (Q5W3B4)
brenda
Schlueter, A.; Szczepanowski, R.; Kurz, N.; Schneiker, S.; Krahn, I.; Puehler, A.
Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function
Appl. Environ. Microbiol.
73
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2007
Escherichia coli (Q47396)
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Nucleotide selectivity of antibiotic kinases
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54
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2010
Escherichia coli (Q47396)
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