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Information on EC 2.7.1.134 - inositol-tetrakisphosphate 1-kinase
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EC Tree
2.7.1.134 inositol-tetrakisphosphate 1-kinaseIUBMB Comments
This enzyme also phosphorylates Ins(1,3,4)P3 on O-5 and O-6. The phosphotransfer from ATP to either inositol 1,3,4-trisphosphate or inositol 3,4,5,6-tetrakisphosphate appears to be freely reversible to the extent that the enzyme can act like an inositol polyphosphate phosphatase in the presence of ADP. It can also catalyse an isomerization between Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP. The enzymes from animals and plants also have the activity of EC 2.7.1.159, inositol-1,3,4-trisphosphate 5/6-kinase.
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Word Map
- 2.7.1.134
- hexakisphosphate
-
2-kinase
-
itpks
- polyphosphate
- 1,3,4,6-tetrakisphosphate
-
atp-grasp
- signalosome
-
phytic
- phytate
- agriculture
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
Synonyms
inositol 1,3,4-trisphosphate 5/6-kinase, osipk, itpk2, ositpk6, hvipk, inositol 1,3,4-trisphosphate 5/6 kinase, inositol tris/tetrakisphosphate kinase 1, inositol-tetrakisphosphate 1-kinase, inositol 1,4,5,6-tetrakisphosphate 3-kinase, inositol 1,3,4-trisphosphate 5/6-kinase 1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1-myo-inositol-trisphosphate 6-kinase
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-
-
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1D-myo-inositol-tetrakisphosphate 1-kinase
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-
-
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1D-myo-inositol-trisphosphate 5-kinase
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-
-
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ATP:1D-myo-inositol-1,3,4-trisphosphate 5-phosphoransferase
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-
-
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ATP:1D-myo-inositol-1,3,4-trisphosphate 6-phosphoransferase
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-
-
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EC 2.7.1.133
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-
formerly
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EC 2.7.1.139
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-
formerly
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inositol 1,3,4-trisphosphate 5/6 kinase
inositol 1,3,4-trisphosphate 5/6-kinase
inositol 1,4,5,6-tetrakisphosphate 3-kinase
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-
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inositol-trisphosphate 5-kinase
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-
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inositol-trisphosphate 6-kinase
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-
-
-
Ipk kinase
ITPK1
ITPK3
kinase, inositol 1,4,5,6-tetrakisphosphate 3- (phosphorylating)
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-
-
-
additional information
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol-3,4,5,6-tetrakisphosphate 1-phosphotransferase
This enzyme also phosphorylates Ins(1,3,4)P3 on O-5 and O-6. The phosphotransfer from ATP to either inositol 1,3,4-trisphosphate or inositol 3,4,5,6-tetrakisphosphate appears to be freely reversible to the extent that the enzyme can act like an inositol polyphosphate phosphatase in the presence of ADP. It can also catalyse an isomerization between Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP. The enzymes from animals and plants also have the activity of EC 2.7.1.159, inositol-1,3,4-trisphosphate 5/6-kinase.
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CAS REGISTRY NUMBER
COMMENTARY
113356-25-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol 1,4,6-trisphosphate
ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ATP + 1D-myo-inositol 3,4,6-trisphosphate
ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
-
-
?
ATP + myo-inositol-1,3,4,6-tetrakisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
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-
r
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,2,3,4-tetrakisphosphate
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-
minor product
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
enzyme ITPK1 has robust enantiospecific hydroxykinase activity against Ins(3,4,5,6)P4
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-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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no activity with inositol-1,3,4,5-tetrakisphosphate
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?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
enzyme has both inositol-3,4,5,6-tetrakisphosphate 1-kinase and inositol-1,3,4-trisphosphate 5/6 kinase activity, 2 times higher activity than with inositol-1,3,4-trisphosphate, no activity with inositol-1,4-bisphosphate, inositol-1,4,5-trisphosphate, inositol-1,3,4,5-tetrakisphosphate, inositol-1,3,4,6-tetrakisphosphate or inositol-1,3,4,5,6-pentakisphosphate
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?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
enzyme has 1-kinase and 1-phosphatase activity as well as phosphomutase activity
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r
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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no activity with D-myo-inositol 1,3,4,5-tetrakisphosphate
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?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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i.e. L-myo-inositol 1,4,5,6-tetrakisphosphate, phosphorylation site: L-3 or D-1 substitution position
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?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
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?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
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?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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no activity with D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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i.e. L-myo-inositol 1,4,5,6-tetrakisphosphate, phosphorylation site: L-3 or D-1 substitution position
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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involved in synthesis of higher phosphorylated inositol phosphates
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?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
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involved in synthesis of higher phosphorylated inositol phosphates
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?
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
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-
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?
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
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-
major product
ir
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
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-
?
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
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-
r
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,6-tetrakisphosphate
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-
-
?
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,6-tetrakisphosphate
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-
minor product
?
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,6-tetrakisphosphate
enzyme has both inositol-1,3,4-trisphosphate 5-kinase and inositol-1,3,4-trisphosphate 6-kinase activity
5:1 ratio of inositol-tetrakis-1,3,4,6-phosphate and inositol-1,2,4,5-tetrakisphosphate
?
ATP + myo-inositol-3,4,6-trisphosphate
ADP + myo-inositol-1,3,4,6-tetrakisphosphate
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-
-
?
ATP + myo-inositol-3,4,6-trisphosphate
ADP + myo-inositol-1,3,4,6-tetrakisphosphate
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-
r
additional information
?
-
the enzyme also performs the reactions of EC 2.7.1.159, phosphorylation of position 5 and/or 6 of 1D-myo-inositol 1,3,4-trisphosphate
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-
?
additional information
?
-
the enzyme also performs the reactions of EC 2.7.1.159, phosphorylation of position 5 and/or 6 of 1D-myo-inositol 1,3,4-trisphosphate
-
-
?
additional information
?
-
-
the enzyme also performs the reactions of EC 2.7.1.159, phosphorylation of position 5 and/or 6 of 1D-myo-inositol 1,3,4-trisphosphate
-
-
?
additional information
?
-
substrate docking calculations, substrate specificity of Arabidopsis thaliana ITPK1, overview. Lack of Ins(1,4,5,6)P4/Ins(3,4,5,6) P4 product for Ins(1,4,6)P3 or Ins(3,4,6)P3 substrates. Consistent with the phosphorylation of the enantiomeric pair Ins(1,4,5,6)P4/Ins (3,4,5,6)P4, ITPK1 shows phosphorylation of Ins(1,4,6)P3 at the 3-position and Ins(3,4,6)P3 at the 1-position. ITPK1 has robust enantiospecific hydroxykinase activity against Ins(3,4,5,6)P4. With inositol tris/tetrakisphosphate kinase 1 (ITPK1, EC 2.7.1.134) and inositol pentakisphosphate 2-kinase (IPK1, EC 2.7.1.158) combined at a 1:20 ATP:ADP ratio, InsP6 is converted via Ins(1,3,4,5,6)P5 to Ins(3,4,5,6) P4 and/or Ins(1,4,5,6)P4 with concomitant generation of ATP
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-
-
additional information
?
-
the enzyme also performs the reactions of EC 2.7.1.159, phosphorylation of position 5 and/or 6 of 1D-myo-inositol 1,3,4-trisphosphate
-
-
?
additional information
?
-
-
phosphate is transferred between inositol phosphates via ITPK1-bound nucleotide. Intersubstrate phosphate transfer explains how competing substrates are able to stimulate each others catalysis by ITPK1
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-
?
additional information
?
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-
Ipk kinase plays a significant role in the inositol phosphate interacting network in plants
-
-
?
additional information
?
-
-
Ipk kinase plays a significant role in the inositol phosphate interacting network in plants
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
involved in synthesis of higher phosphorylated inositol phosphates
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
involved in synthesis of higher phosphorylated inositol phosphates
-
?
additional information
?
-
-
phosphate is transferred between inositol phosphates via ITPK1-bound nucleotide. Intersubstrate phosphate transfer explains how competing substrates are able to stimulate each others catalysis by ITPK1
-
-
?
additional information
?
-
-
Ipk kinase plays a significant role in the inositol phosphate interacting network in plants
-
-
?
additional information
?
-
-
Ipk kinase plays a significant role in the inositol phosphate interacting network in plants
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
inositol-1,3,4-trisphosphate
activation of inositol-1,3,4,5,6-phosphate 1-phosphatase activity
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Dehydration
OsITL1 gene encoding an inositol 1,3,4-trisphosphate 5/6-kinase is a negative regulator of osmotic stress signaling.
Neoplasms
Increased levels of inositol hexakisphosphate (InsP6) protect HEK293 cells from tumor necrosis factor (alpha)- and Fas-induced apoptosis.
Neoplasms
Inositol 1,3,4-trisphosphate 5/6-kinase inhibits tumor necrosis factor-induced apoptosis.
Neural Tube Defects
Expression of inositol 1,3,4-trisphosphate 5/6-kinase (ITPK1) and its role in neural tube defects.
Neural Tube Defects
Neural tube defects in mice with reduced levels of inositol 1,3,4-trisphosphate 5/6-kinase.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0001
myo-inositol-3,4,5,6-tetrakisphosphate
pH 7.2, 37°C, recombinant enzyme
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
expression pattern of isozyme ITPK2, no or very poor expression in stem
brenda
3.6 kilobases transcript detected by Northern blot
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
substrate/product analysis using anion exchange chromatography on an acid- and hydroxide-stable quaternary amine functionalized latex, substrate docking calculations, resolvement and measurement of diphosphoinositol phosphates beside lower inositol phosphates without the use of radiolabel, method, overview. Modeling of the binding of the enantiomers Ins(1,4,5,6)P4 and Ins(3,4,5,6)P4 to ITPK1. Significant interaction of the 4-phosphate of Ins(1,4,5,6)P4 and of the 6-phosphate of the Ins(3,4,5,6)P4 in site F. The respective 1- and 3-phosphates of Ins(1,4,5,6)P4 and Ins(3,4,5,6)P4 make contacts in site C. These subsites are likely determinants of the reactivity of ITPK1
evolution
Arabidopsis thaliana ITPK1, belongs to a class of enzyme that has been shown to possess 1-, 5- and 6-hydroxykinase and (InsP6) 5-phosphokinase activities
evolution
OsITPK6 belongs to subgroup III, with 12 exons and 11 introns, of the OsITPK gene family. ITPK6 is a unique gene in the ITPK gene family
malfunction