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Information on EC 2.7.1.121 - phosphoenolpyruvate-glycerone phosphotransferase
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2.7.1.121 phosphoenolpyruvate-glycerone phosphotransferaseSpecify your search results
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dhal
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
dhaklm, pep-dependent dihydroxyacetone kinase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DhaKLM
PEP-dependent dihydroxyacetone kinase
phosphotransferase, phosphohistidinoprotein-dihydroxyacetone
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
phosphoenolpyruvate:glycerone phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY
91755-81-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
phosphohistidinoprotein + D-fructose
histidine-containing protein + ?
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enzyme II complex
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-
?
phosphohistidinoprotein + D-galactitol
histidine-containing protein + D-galactitol phosphate
phosphohistidinoprotein + D-glucose
histidine-containing protein + D-glucose phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + D-mannose
histidine-containing protein + D-mannose phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + dihydroxyacetone
histidine-containing protein + dihydroxyacetone phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + N-acetyl-D-glucosamine
histidine-containing protein + N-acetyl-D-glucosamine phosphate
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enzyme II complex
-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
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-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
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overall reaction of phosphotransferase system, involved in glycerol dissimilation of E. coli
enzyme II complex
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
glycerone is dihydroxyacetone
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-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
-
-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
glycerone is dihydroxyacetone
-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
-
-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
glycerone is dihydroxyacetone
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-
?
phosphoenolpyruvate + histidine-containing protein
pyruvate + phosphohistidinoprotein
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enzyme I
-
-
?
phosphoenolpyruvate + histidine-containing protein
pyruvate + phosphohistidinoprotein
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enzyme I
-
-
?
phosphohistidinoprotein + D-galactitol
histidine-containing protein + D-galactitol phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + D-galactitol
histidine-containing protein + D-galactitol phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + D-glucitol
histidine-containing protein + D-glucitol phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + D-glucitol
histidine-containing protein + D-glucitol phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + D-mannitol
histidine-containing protein + D-mannitol phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + D-mannitol
histidine-containing protein + D-mannitol phosphate
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enzyme II complex
-
-
?
phosphohistidinoprotein + glycerone
histidine-containing protein + glycerone phosphate
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i.e. dihydroxyacetone, enzyme II complex
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-
?
phosphohistidinoprotein + glycerone
histidine-containing protein + glycerone phosphate
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i.e. dihydroxyacetone, enzyme II complex
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-
?
additional information
?
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glycerone (DHA) is an unstable compound that can be interconverted into different forms when dissolved in water or autoxidized by Fenton's reaction to form glycolate, other short-chained carbohydrates, and organic acids upon incubation. NMR analysis of DHA stability at 37°C
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additional information
?
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glycerone (DHA) is an unstable compound that can be interconverted into different forms when dissolved in water or autoxidized by Fenton's reaction to form glycolate, other short-chained carbohydrates, and organic acids upon incubation. NMR analysis of DHA stability at 37°C
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additional information
?
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glycerone (DHA) is an unstable compound that can be interconverted into different forms when dissolved in water or autoxidized by Fenton's reaction to form glycolate, other short-chained carbohydrates, and organic acids upon incubation. NMR analysis of DHA stability at 37°C
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
-
-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
-
overall reaction of phosphotransferase system, involved in glycerol dissimilation of E. coli
enzyme II complex
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
-
-
-
?
phosphoenolpyruvate + glycerone
pyruvate + glycerone phosphate
-
-
-
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.006
Glycerone
below, pH and temperature not specified in the publication
additional information
additional information
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30°C, enzyme II, 0.003-0.004 mM for mannitol, glucitol and galactitol depending on substrate and 0.01-0.05 mM for glucose and mannose depending on substrate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4.8
Glycerone
below, pH and temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
800
Glycerone
below, pH and temperature not specified in the publication
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
under aerobic conditions, Escherichia coli growth on dihydroxyacetone (DHA) is far from optimal and is hindered by chemical, hierarchical, and possibly allosteric constraints. Optimal growth on DHA can be restored by releasing the hierarchical constraint. Escherichia coli growth on DHA is robust but suboptimal
brenda
additional information
-
under aerobic conditions, Escherichia coli growth on dihydroxyacetone (DHA) is far from optimal and is hindered by chemical, hierarchical, and possibly allosteric constraints. Optimal growth on DHA can be restored by releasing the hierarchical constraint. Escherichia coli growth on DHA is robust but suboptimal
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brenda
additional information
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under aerobic conditions, Escherichia coli growth on dihydroxyacetone (DHA) is far from optimal and is hindered by chemical, hierarchical, and possibly allosteric constraints. Optimal growth on DHA can be restored by releasing the hierarchical constraint. Escherichia coli growth on DHA is robust but suboptimal
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brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
malfunction
dhaK transposon insertion prevents the growth of Escherichia coli on DHA indicates that DhaKLM reaction is important. Inactivation of dhaKLM is not lethal. Overexpression of the GLD and FSA pathways leads to optimal growth on DHA
malfunction
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dhaK transposon insertion prevents the growth of Escherichia coli on DHA indicates that DhaKLM reaction is important. Inactivation of dhaKLM is not lethal. Overexpression of the GLD and FSA pathways leads to optimal growth on DHA
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malfunction
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dhaK transposon insertion prevents the growth of Escherichia coli on DHA indicates that DhaKLM reaction is important. Inactivation of dhaKLM is not lethal. Overexpression of the GLD and FSA pathways leads to optimal growth on DHA
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metabolism
Escherichia coli can metabolize DHA aerobically through at least three different metabolic pathways: (i) the dihydroxyacetone kinase (DAK) pathway, (ii) the glycerol (GLD) pathway, and (iii) the fructose-6-phosphate (FSA) pathway. The DAK pathway is named after dihydroxyacetone (DHA) kinase, encoded by the dhaKLM operon. This operon is controlled by DhaR, a transcription factor activated by DHA. DhaKLM is composed of three subunits (DhaK, DhaL, and DhaM) and phosphorylates DHA to dihydroxyacetone phosphate (DHAP), an intermediate in the glycolytic pathway. This kinase resembles a phosphotransferase system (PTS) that uses phosphoenolpyruvate (PEP) as a phosphoryl donor. Experimental and simulated fluxes through DHA metabolism of Escherichia coli, analysis of the metabolic regulation and function of DHA, overview. The DAK pathway is central but not essential for DHA metabolism. The GLD and FSA pathways are functionally involved in DHA metabolism
metabolism
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Escherichia coli can metabolize DHA aerobically through at least three different metabolic pathways: (i) the dihydroxyacetone kinase (DAK) pathway, (ii) the glycerol (GLD) pathway, and (iii) the fructose-6-phosphate (FSA) pathway. The DAK pathway is named after dihydroxyacetone (DHA) kinase, encoded by the dhaKLM operon. This operon is controlled by DhaR, a transcription factor activated by DHA. DhaKLM is composed of three subunits (DhaK, DhaL, and DhaM) and phosphorylates DHA to dihydroxyacetone phosphate (DHAP), an intermediate in the glycolytic pathway. This kinase resembles a phosphotransferase system (PTS) that uses phosphoenolpyruvate (PEP) as a phosphoryl donor. Experimental and simulated fluxes through DHA metabolism of Escherichia coli, analysis of the metabolic regulation and function of DHA, overview. The DAK pathway is central but not essential for DHA metabolism. The GLD and FSA pathways are functionally involved in DHA metabolism
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metabolism
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Escherichia coli can metabolize DHA aerobically through at least three different metabolic pathways: (i) the dihydroxyacetone kinase (DAK) pathway, (ii) the glycerol (GLD) pathway, and (iii) the fructose-6-phosphate (FSA) pathway. The DAK pathway is named after dihydroxyacetone (DHA) kinase, encoded by the dhaKLM operon. This operon is controlled by DhaR, a transcription factor activated by DHA. DhaKLM is composed of three subunits (DhaK, DhaL, and DhaM) and phosphorylates DHA to dihydroxyacetone phosphate (DHAP), an intermediate in the glycolytic pathway. This kinase resembles a phosphotransferase system (PTS) that uses phosphoenolpyruvate (PEP) as a phosphoryl donor. Experimental and simulated fluxes through DHA metabolism of Escherichia coli, analysis of the metabolic regulation and function of DHA, overview. The DAK pathway is central but not essential for DHA metabolism. The GLD and FSA pathways are functionally involved in DHA metabolism
-
physiological function
Escherichia coli can metabolize DHA aerobically through at least three different metabolic pathways: (i) the dihydroxyacetone kinase (DAK) pathway, (ii) the glycerol (GLD) pathway, and (iii) the fructose-6-phosphate (FSA) pathway. The DAK pathway is named after dihydroxyacetone (DHA) kinase, encoded by the dhaKLM operon. This operon is controlled by DhaR, a transcription factor activated by DHA. DhaKLM is composed of three subunits (DhaK, DhaL, and DhaM) and phosphorylates DHA to dihydroxyacetone phosphate (DHAP), an intermediate in the glycolytic pathway
physiological function
-
Escherichia coli can metabolize DHA aerobically through at least three different metabolic pathways: (i) the dihydroxyacetone kinase (DAK) pathway, (ii) the glycerol (GLD) pathway, and (iii) the fructose-6-phosphate (FSA) pathway. The DAK pathway is named after dihydroxyacetone (DHA) kinase, encoded by the dhaKLM operon. This operon is controlled by DhaR, a transcription factor activated by DHA. DhaKLM is composed of three subunits (DhaK, DhaL, and DhaM) and phosphorylates DHA to dihydroxyacetone phosphate (DHAP), an intermediate in the glycolytic pathway
-
physiological function
-
Escherichia coli can metabolize DHA aerobically through at least three different metabolic pathways: (i) the dihydroxyacetone kinase (DAK) pathway, (ii) the glycerol (GLD) pathway, and (iii) the fructose-6-phosphate (FSA) pathway. The DAK pathway is named after dihydroxyacetone (DHA) kinase, encoded by the dhaKLM operon. This operon is controlled by DhaR, a transcription factor activated by DHA. DhaKLM is composed of three subunits (DhaK, DhaL, and DhaM) and phosphorylates DHA to dihydroxyacetone phosphate (DHAP), an intermediate in the glycolytic pathway
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
the enzyme consists of subunits dhaL, dhaK, and dhaM
additional information
-
the enzyme consists of subunits dhaL, dhaK, and dhaM
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additional information
-
the enzyme consists of subunits dhaL, dhaK, and dhaM
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
construction of a DHALKM deletion mutant strain, analysis of the change in gene expression in DHA metabolism of the wild-type strain and DELTAdhaKLM strain cultured on modified M9-DHA medium, overview. Optimal growth on DHA can be achieved by releasing hierarchical constraints on DHA metabolism, opening additional routes for its assimilation
additional information
-
construction of a DHALKM deletion mutant strain, analysis of the change in gene expression in DHA metabolism of the wild-type strain and DELTAdhaKLM strain cultured on modified M9-DHA medium, overview. Optimal growth on DHA can be achieved by releasing hierarchical constraints on DHA metabolism, opening additional routes for its assimilation
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additional information
-
construction of a DHALKM deletion mutant strain, analysis of the change in gene expression in DHA metabolism of the wild-type strain and DELTAdhaKLM strain cultured on modified M9-DHA medium, overview. Optimal growth on DHA can be achieved by releasing hierarchical constraints on DHA metabolism, opening additional routes for its assimilation
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jin, R.Z.; Lin, E.C.C.
An inducible phosphoenolpyruvate: dihydroxyacetone phosphotransferase system in Escherichia coli
J. Gen. Microbiol.
130
83-88
1984
Escherichia coli, Escherichia coli ECL1
brenda
Peiro, C.; Millard, P.; de Simone, A.; Cahoreau, E.; Peyriga, L.; Enjalbert, B.; Heux, S.
Chemical and metabolic controls on dihydroxyacetone metabolism lead to suboptimal growth of Escherichia coli
Appl. Environ. Microbiol.
85
e00768-19
2019
Escherichia coli (P76014 AND P76015 AND P37349), Escherichia coli BW25113 (P76014 AND P76015 AND P37349), Escherichia coli K12 (P76014 AND P76015 AND P37349)
brenda
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