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Information on EC 2.7.1.113 - deoxyguanosine kinase and Organism(s) Mus musculus and UniProt Accession Q9QX60

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EC Tree
IUBMB Comments
Deoxyinosine can also act as acceptor.
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This record set is specific for:
Mus musculus
UNIPROT: Q9QX60
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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ATP
+
deoxyguanosine
=
ADP
+
dGMP
+
=
+
Synonyms
deoxyguanosine kinase, dguok, 2'-deoxyguanosine kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(dihydroxypropoxymethyl)guanine kinase
-
-
-
-
2'-deoxyguanosine kinase
-
-
-
-
kinase, deoxyguanosine (phosphorylating)
-
-
-
-
NTP-deoxyguanosine 5'-phosphotransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:deoxyguanosine 5'-phosphotransferase
Deoxyinosine can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
39471-28-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2',2'-difluorodeoxyguanosine
ADP + 2',2'-difluorodeoxyguanosine 5'-monophosphate
show the reaction diagram
recombinant mitochondrial deoxyguanosine kinase, 147% of activity with deoxyguanosine
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
show the reaction diagram
recombinant mitochondrial deoxyguanosine kinase, 200% of activity with deoxyguanosine at 0.005 mM
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
recombinant mitochondrial deoxyguanosine kinase, very low activity
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
?
ATP + deoxyinosine
ADP + dIMP
show the reaction diagram
10% of activity with deoxyguanosine
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
CTP + deoxyguanosine
CDP + dGMP
show the reaction diagram
-
64% of activity with ATP
-
?
dCTP + deoxyguanosine
dCDP + dGMP
show the reaction diagram
-
35% of activity with ATP
-
?
dTTP + deoxyguanosine
dTDP + dGMP
show the reaction diagram
-
84% of activity with ATP
-
?
UTP + deoxyguanosine
UDP + dGMP
show the reaction diagram
-
20% of activity with ATP
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
first reaction in reutilization of deoxyguanosine for dGTP biosynthesis
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation
Cd2+
-
slight activation
Mg2+
-
required for activity
Mn2+
-
can replace Mg2+ to some extent
Zn2+
-
activation, can replace Mg2+ to some extent
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
deoxyadenosine
-
0.07 mM, 9% inhibition
deoxyinosine
-
0.07 mM, 17% inhibition
dGDP
-
0.1 mM, 97% inhibition
dGMP
-
-
dGTP
-
0.1 mM, 99% inhibition
dTDP
-
-
UDP
-
strong inhibition
additional information
-
not inhibited by deoxyadenosine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023 - 0.63
ATP
0.007
deoxyguanosine
-
pH 5.2, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007
dGDP
-
pH 5.2, 37°C, competitive vs. MgATP2-, at MgATP2- concentrations above 0.5 mM
0.18
dGMP
-
pH 5.2, 37°C, competitive vs. deoxyguanosine
0.00007 - 0.0019
dGTP
0.003
UDP
-
pH 5.2, 37°C, competitive vs. MgATP2-, at MgATP2- concentrations above 0.5 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0007
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
substrate deoxyguanosine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 9
-
approx. 20% of maximal activity at pH 4.5 and pH 6.5 respectively, approx. 10% of maximal activity at pH 9.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low expression levels
Manually annotated by BRENDA team
low expression
Manually annotated by BRENDA team
high expression levels
Manually annotated by BRENDA team
-
neonatal skin tissue
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
deoxyguanosine kinase 2
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DGUOK_MOUSE
277
0
32281
Swiss-Prot
Mitochondrion (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
glycerol density gradient centrifugation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ATP or MgATP2- stabilize, not Mg2+ alone
-
loss of activity in dilute solution
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, ammonium sulfate precipitate in 100 mM M Tris-acetate buffer, pH 8, 0.025 M 2-mercaptoethanol, at least 4 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
pH 5.7, streptomycin sulfate, ammonium sulfate
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of His-tagged deoxyguanosine kinase in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Barker, J.; Lewis, R.A.
Deoxyguanosine kinase of neonatal mouse skin tissue
Biochim. Biophys. Acta
658
111-123
1981
Mus musculus
Manually annotated by BRENDA team
Petrakis, T.G.; Ktistaki, E.; Wang, L.; Eriksson, S.; Talianidis, I.
Cloning and characterization of mouse deoxyguanosine kinase. Evidence for a cytoplasmic isoform
J. Biol. Chem.
274
24726-24730
1999
Mus musculus (Q9QX60), Mus musculus
Manually annotated by BRENDA team