Any feedback?
Information on EC 2.7.1.113 - deoxyguanosine kinase
for references in articles please use BRENDA:EC2.7.1.113Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.1.113 deoxyguanosine kinaseIUBMB Comments
Deoxyinosine can also act as acceptor.
Specify your search results
Word Map
- 2.7.1.113
- deoxycytidine
- mtdna
- thymidine
-
hepatocerebral
- deoxyribonucleoside
- deoxynucleoside
- ophthalmoplegia
- sucla2
- twinkle
- 9-beta-d-arabinofuranosylguanine
- cladribine
- c10orf2
-
nystagmus
- 2-chlorodeoxyadenosine
- deoxyinosine
-
2.7.1.74
- 2-chloro-2'-deoxyadenosine
-
mngie
-
neurogastrointestinal
- medicine
The enzyme appears in viruses and cellular organisms
Synonyms
deoxyguanosine kinase, dguok, 2'-deoxyguanosine kinase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(dihydroxypropoxymethyl)guanine kinase
-
-
-
-
2'-deoxyguanosine kinase
-
-
-
-
deoxyadenosine kinase/deoxyguanosine kinase
deoxyguanosine kinase
DGK
dGKase
DGUOK
kinase, deoxyguanosine (phosphorylating)
-
-
-
-
NTP-deoxyguanosine 5'-phosphotransferase
-
-
-
-
nucleoside triphosphate: deoxyguanosine 5'-phosphotransferase
deoxyadenosine kinase/deoxyguanosine kinase
abbrevation: dAK/dGK
deoxyadenosine kinase/deoxyguanosine kinase
abbrevation: dAK/dGK
-
DGK
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + deoxyguanosine = ADP + dGMP
active site residues are Glu70, Lys142, and Arg142
-
ATP + deoxyguanosine = ADP + dGMP
substrate interactions and binding mechanism, and active site structure
ATP + deoxyguanosine = ADP + dGMP
substrate interactions, reaction mechanism
ATP + deoxyguanosine = ADP + dGMP
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
ATP:deoxyguanosine 5'-phosphotransferase
Deoxyinosine can also act as acceptor.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
39471-28-8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxyguanosine
2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxyguanosine
2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxyguanosine 5'-phosphate
-
low activity
-
-
?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxyguanosine
2'-deoxyadenosine-3'-diphosphate + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine-2'-triphosphate + 2'-deoxyguanosine
3'-deoxyadenosine-2'-diphosphate + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxyguanosine
3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxyguanosine
3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
ADP + ?
-
4.4% of the activity with 2'-deoxyguanosine
-
-
?
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril
ADP + ?
-
poor substrate, 1.1% of the activity with 2'-deoxyguanosine
-
-
?
ATP + 1-beta-D-arabinofuranosyladenine
ADP + 1-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
ATP + 2',2'-difluorodeoxyguanosine
ADP + 2',2'-difluorodeoxyguanosine 5'-monophosphate
recombinant mitochondrial deoxyguanosine kinase, 147% of activity with deoxyguanosine
-
?
ATP + 2',2'-difluorodeoxyguanosine
ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxyguanosine
ADP + 2',3'-dideoxyguanosine 5'-monophosphate
-
recombinant deoxyguanosine kinase, 14% of activity with deoxyguanosine
-
?
ATP + 2'-deoxyinosine
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
ATP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
ADP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine 5'-monophosphate
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine
ADP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-monophosphate
-
-
-
-
?
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2-fluoro-arabinosyl-adenine
ADP + 2-fluoro-arabinosyl-adenine 5'-monophosphate
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + 2-fluoroadenine-beta-D-arabinofuranoside
ADP + 2-fluoroadenine-beta-D-arabinofuranoside 5'-monophosphate
-
-
-
-
?
ATP + 3'-fluoro-2',3'-dideoxyguanosine
ADP + 3'-fluoro-2',3'-dideoxyguanosine 5'-monophosphate
-
recombinant deoxyguanosine kinase, 14% of activity with deoxyguanosine
-
?
ATP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine
ADP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine 5'-monophosphate
-
antiherpesvirus drug ganciclovir, recombinant mitochondrial deoxyguanosine kinase, 6% of activity with deoxyguanosine
-
?
ATP + 9-(4-hydroxy-3-hydroxymethylbutyl-1-yl)guanine
ADP + 9-(4-hydroxy-3-hydroxymethylbutyl-1-yl)guanine 5'-monophosphate
-
antiherpesvirus drug penciclovir, recombinant mitochondrial deoxyguanosine kinase, 50% of activity with deoxyguanosine
-
?
ATP + 9-beta-D-arabinofuranosylguanine
ADP + 9-beta-D-arabinofuranosylguanine 5'-monophosphate
ATP + 9-beta-D-arabinofuranosylguanine
ADP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
ATP + 9-beta-D-arabinosylguanine
ADP + 9-beta-D-arabinosylguanine 5'-monophosphate
-
-
-
?
ATP + arabinosyl adenine
ADP + arabinosyl adenine 5'-monophosphate
-
recombinant mitochondrial deoxyguanosine kinase, very low activity
-
?
ATP + arabinosyl guanine
ADP + arabinosyl guanine 5'-monophosphate
-
recombinant mitochondrial deoxyguanosine kinase, better substrate than deoxyguanosine kinase
-
?
ATP + dideoxyinosine
ADP + dideoxyinosine 5'-monophosphate
-
recombinant mitochondrial deoxyguanosine kinase, very low activity
-
?
ATP + ganciclovir
ADP + ganciclovir phosphate
i.e. 9-(1,3-dihydroxy-2-propoxymethyl)-guanine, low activity
-
-
?
ATP + penciclovir
ADP + penciclovir 5'-phosphate
i.e. 9-(2-hydroxy-1-hydroxymethyl-ethoxymethyl)-guanine, low activity
-
-
?
dUTP + deoxyguanosine
dUDP + dGMP
-
69% of activity with ATP at pH 6.0, 1.7fold higher activity than with ATP at pH 7.0
-
?
GTP + 2'-deoxyadenosine
GDP + 2'-deoxyadenosine 5'-phosphate
-
very low activity
-
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxycytidine 5'-phosphate
-
very low activity
-
-
?
triphosphate + 2'-deoxyguanosine
diphosphate + 2'-deoxyguanosine 5'-phosphate
-
triphosphate is active with 2'deoxyguanosine, 50% of the activity with ATP
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
UTP + 9-beta-D-arabinofuranosylguanine
UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
i.e. nelarabine, activation of the anticancer drug
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
key enzyme in mitochondrial DNA precursor synthesis
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
key enzyme in mitochondrial DNA precursor synthesis
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
important enzyme for the phosphorylation/activation of 2'-deoxyguanosine
-
-
ir
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
one of the two mitochondrial deoxynucleoside salvage pathway enzymes involved in precursor synthesis for mitochondrial DNA replication, DGUOK is responsible for the initial rate-limiting phosphorylation of the purine deoxynucleosides, mutations of the enzyme are associated with the hepato-specific and hepatocerebral forms of the mtDNA depletion syndrome
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
-
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
recombinant mitochondrial deoxyguanosine kinase, 200% of activity with deoxyguanosine at 0.005 mM
-
?
ATP + 9-beta-D-arabinofuranosylguanine
ADP + 9-beta-D-arabinofuranosylguanine 5'-monophosphate
-
-
-
-
?
ATP + 9-beta-D-arabinofuranosylguanine
ADP + 9-beta-D-arabinofuranosylguanine 5'-monophosphate
-
guanosine analog with activity in patients with T-cell malignancies
-
?
ATP + deoxyadenosine
ADP + dAMP
barely measurable phosphorylation of deoxyadenosine
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + deoxyadenosine
ADP + dAMP
-
4.8%, 25.8% and 93.5% of activity with deoxyguanosine at 0.01 mM, 0.1 mM and 1 mM deoxyguanosine, respectively
-
?
ATP + deoxyadenosine
ADP + dAMP
recombinant mitochondrial deoxyguanosine kinase, very low activity
-
?
ATP + deoxycytidine
ADP + dCMP
-
recombinant deoxyguanosine kinase, 3% of activity with deoxyguanosine
-
-
?
ATP + deoxycytidine
ADP + dCMP
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + deoxyguanosine
ADP + dGMP
-
poor donor substrates are dATP or GTP
-
?
ATP + deoxyguanosine
ADP + dGMP
-
no activity with inosine and 9-beta-arabinofuranosyladenine
-
?
ATP + deoxyguanosine
ADP + dGMP
-
no activity with deoxythymidine, uridine and cytosine
-
?
ATP + deoxyguanosine
ADP + dGMP
-
ATP most efficient at pH 5.5, dTTP or UTP most efficient, i.e. more efficient than ATP, at pH 7.4
-
?
ATP + deoxyguanosine
ADP + dGMP
-
ATP can be replaced by dTTP, CTP, dCTP and UTP
-
?
ATP + deoxyguanosine
ADP + dGMP
-
highly specific for phospho group acceptor
-
?
ATP + deoxyguanosine
ADP + dGMP
-
enzyme of purine deoxynucleoside salvage pathway
-
?
ATP + deoxyguanosine
ADP + dGMP
-
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
recombinant mitochondrial deoxyguanosine kinase does not discriminate between beta-D-2'-deoxyguanosine and beta-L-2'-deoxyguanosine
-
?
ATP + deoxyguanosine
ADP + dGMP
-
ATP can be replaced by dTTP, CTP, dCTP and UTP
-
?
ATP + deoxyguanosine
ADP + dGMP
-
no activity with deoxythymidine, uridine and cytosine
-
?
ATP + deoxyguanosine
ADP + dGMP
-
ATP can be replaced by dTTP, CTP, dCTP and UTP
-
?
ATP + deoxyguanosine
ADP + dGMP
-
84%, 64%, 35% and 20% of activity with ATP with dTTP, CTP, dCTP and UTP, respectively
-
?
ATP + deoxyguanosine
ADP + dGMP
-
first reaction in reutilization of deoxyguanosine for dGTP biosynthesis
-
?
ATP + deoxyguanosine
ADP + dGMP
-
highly specific for phospho group acceptor
-
?
ATP + deoxyguanosine
ADP + dGMP
-
ATP twice as effective as CTP or GTP and 4 times as effective as UTP
-
?
ATP + deoxyinosine
ADP + dIMP
-
63% of activity with deoxyguanosine
-
?
ATP + deoxyinosine
ADP + dIMP
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + deoxyinosine
ADP + dIMP
-
recombinant mitochondrial deoxyguanosine kinase, may be the preferred substrate in vivo
-
?
ATP + deoxyinosine
ADP + dIMP
10% of activity with deoxyguanosine
-
?
ATP + dioxolane guanosine
ADP + dioxolane guanosine 5'-phosphate
-
low activity, step in anabolism of amdoxovir, i.e. (-)-beta-D-2,6-diaminopurine dioxolane, a prodrug of dioxolane guanosine
-
-
?
ATP + dioxolane guanosine
ADP + dioxolane guanosine 5'-phosphate
-
0.02% of the activity with 2'-deoxyguanosine
-
-
?
ATP + guanosine
ADP + GMP
-
recombinant deoxyguanosine kinase, 7% of activity with deoxyguanosine
-
?
CTP + deoxyguanosine
CDP + dGMP
-
62% of activity with ATP at pH 6.0, 1.8fold higher activity than with ATP at pH 7.0
-
?
dTTP + deoxyguanosine
dTDP + dGMP
-
83% of activity with ATP at pH 6.0, 2.8fold higher activity than with ATP at pH 7.0
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
-
preferred substrates
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
additional information
?
-
-
substrate specificity, the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors, and shows poor activity with thymidine, inosine, adenosine, 2,3-dideoxyguanosine, 2,3-dideoxyinosine, 2,3-dideoxyadenosine, and hypoxanthine arabinoside as acceptor substrates
-
-
?
additional information
?
-
the enzyme has low specificity for guanosine
-
-
?
additional information
?
-
the enzyme has low specificity for guanosine
-
-
?
additional information
?
-
-
no activity with diphosphate as phosphate donor
-
-
?
additional information
?
-
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview
-
-
?
additional information
?
-
-
enzyme defect due to genetic alterations leads to tissue-specific mitochondrial DNA depletion causing multi-systemic disease or devastating liver disease
-
-
?
additional information
?
-
-
th enzyme catalyzes the initial phosphorylation of purine deoxynucleosides, enzyme deficiency leads to severe mitochondrial DNA depletion diseases
-
-
?
additional information
?
-
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
-
-
?
additional information
?
-
anticancer drugs 2-chloro-2'-deoxyadenosine, i.e. cladribine, and 2-fluoro-9-beta-D-arabinofuranosyladenine, i.e. clofarabine, are poor substrates
-
-
?
additional information
?
-
-
no activity with several difluorophenyl nucleoside analogues, overview
-
-
?
additional information
?
-
substrate interactions, overview, enzyme shows relaxed enantioselectivity, accepting D- and L-enantiomers of beta-deoxyguanosine but with lower activity than alpha-L-deoxyguanosine, and beta-L-deoxyadenosine, enzyme accepts purine deoxynucleosides and analogues as substrates utilizing a triphosphate donor substrate
-
-
?
additional information
?
-
the enzyme is highly specific for purine substrates, Arg118 is responsible for the substrate specificity
-
-
?
additional information
?
-
-
the enzyme is highly specific for purine substrates, Arg118 is responsible for the substrate specificity
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + deoxyinosine
ADP + dIMP
-
recombinant mitochondrial deoxyguanosine kinase, may be the preferred substrate in vivo
-
?
ATP + dioxolane guanosine
ADP + dioxolane guanosine 5'-phosphate
-
low activity, step in anabolism of amdoxovir, i.e. (-)-beta-D-2,6-diaminopurine dioxolane, a prodrug of dioxolane guanosine
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
key enzyme in mitochondrial DNA precursor synthesis
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
key enzyme in mitochondrial DNA precursor synthesis
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
important enzyme for the phosphorylation/activation of 2'-deoxyguanosine
-
-
ir
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
one of the two mitochondrial deoxynucleoside salvage pathway enzymes involved in precursor synthesis for mitochondrial DNA replication, DGUOK is responsible for the initial rate-limiting phosphorylation of the purine deoxynucleosides, mutations of the enzyme are associated with the hepato-specific and hepatocerebral forms of the mtDNA depletion syndrome
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
enzyme of purine deoxynucleoside salvage pathway
-
?
ATP + deoxyguanosine
ADP + dGMP
-
first reaction in reutilization of deoxyguanosine for dGTP biosynthesis
-
?
additional information
?
-
the enzyme has low specificity for guanosine
-
-
?
additional information
?
-
the enzyme has low specificity for guanosine
-
-
?
additional information
?
-
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview