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Information on EC 2.7.1.108 - dolichol kinase and Organism(s) Arabidopsis thaliana and UniProt Accession F4J4C8

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Arabidopsis thaliana
UNIPROT: F4J4C8 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
Synonyms
dolichol kinase, sec59, atdok1, dolichol phosphokinase, at3g45040, dolichol kinase 1, sec59p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dolichol kinase 1
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dolichol phosphokinase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
CTP:dolichol O-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
71768-07-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + dolichol
CDP + dolichyl phosphate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + dolichol
CDP + dolichyl phosphate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
DOK1 is highly expressed in tapetum cells and microspores during early anther development
Manually annotated by BRENDA team
floral meristem
Manually annotated by BRENDA team
high expression level
Manually annotated by BRENDA team
root tip of germinating seedlings, and in the founder cells where lateral root growth is initiated
Manually annotated by BRENDA team
apical meristem
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
topology of AtDOK1 in the endoplasmic reticulum membrane, overview. AtDOK1 contains 13 transmembrane (TM) segments (TM1-TM13) with an N-terminus facing the endoplasmic reticulum lumen and a C-terminus facing the cytoplasm. The topological arrangement of AtDOK1 is similar to that of human enzyme
Manually annotated by BRENDA team
additional information
subcellular localization study
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
the heterozygous T-DNA-tagged AtDOK1 mutant, dok1-1 and dok1-2, plants show developmental defects in male and female gametophytes, including an aberrant pollen structure, low pollen viability, and short siliques. Additionally, the mutations has incomplete penetrance. Mutation of dok-1 may affect development of siliques but not seed maturation or germination in Arabidopsis thaliana. Male gametophytic defect in dok1 heterozygous plants, overview
physiological function
additional information
AtDOK1 contains a DxxAxxxGxxxGx8KKTxEG motif that is conserved among enzymes utilizing CTP as a substrate, including hDOLK and yeast Sec59p. The motif contains the CTP binding site
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EVN_ARATH
569
13
63136
Swiss-Prot
Secretory Pathway (Reliability: 4)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G471D
site-directed mutagenesis,inactive mutant
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene At3g45040 or AtDOK1, DNA and amino acid sequence determination and analysis, a cytidylyltransferase motif-containing gene, phylogenetic tree. The gene complements defects in the growth and N-linked glycosylation of the Saccharomyces cerevisiae sec59 mutant, deficient in dolichol kinase Sec59p
gene SEC59, Agrobacterium tumefaciens strain GV3101 is used to transform dok1-1 heterozygous plants with the Venus-tagged wild-type and mutant enzymes (DOK1-Venus fusion proteins), functional complementation of the Saccharomyces cerevisiae sec59 mutant, showing the temperature-sensitive growth phenotype, by expression of the His6-tagged Arabidopsis thaliana enzyme, the sec59 mutant expressing AtDOK1 (G741D) mutant fails to do so, quantitative RT-PCR expression analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakamura, Y.
Arabidopsis DOK1 encodes a functional dolichol kinase involved in reproduction
Plant J.
81
292-303
2015
Arabidopsis thaliana (F4J4C8), Arabidopsis thaliana
Manually annotated by BRENDA team
Cho, Y.; Yu, C.Y.; Nakamura, Y.; Kanehara, K.
Arabidopsis dolichol kinase AtDOK1 is involved in flowering time control
J. Exp. Bot.
68
3243-3252
2017
Arabidopsis thaliana (F4J4C8)
Manually annotated by BRENDA team