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Information on EC 2.7.1.105 - 6-phosphofructo-2-kinase and Organism(s) Bos taurus and UniProt Accession P26285

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EC Tree
IUBMB Comments
Not identical with EC 2.7.1.11 6-phosphofructokinase. The enzyme co-purifies with EC 3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase.
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This record set is specific for:
Bos taurus
UNIPROT: P26285
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Synonyms
6-phosphofructo-2-kinase, phosphofructokinase 2, phosphofructokinase-2, upfk-2, inducible 6-phosphofructo-2-kinase, fructose 6-phosphate 2-kinase, ipfk2, tpfk-2, phosphofructokinase-2/fructose bisphosphatase-2, 6-phosphofructose 2-kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase
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-
-
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6-phosphofructose 2-kinase
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-
-
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ATP:D-fructose-6-phosphate 2-phosphotransferase
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-
-
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fructose 6-phosphate 2-kinase
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-
-
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kinase, 6-phosphofructo-2-(phosphorylating)
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-
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phosphofructokinase 2
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-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
show the reaction diagram
bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ATP:beta-D-fructose-6-phosphate 2-phosphotransferase
Not identical with EC 2.7.1.11 6-phosphofructokinase. The enzyme co-purifies with EC 3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase.
CAS REGISTRY NUMBER
COMMENTARY hide
78689-77-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + beta-D-fructose 6-phosphate
ADP + beta-D-fructose 2,6-bisphosphate
show the reaction diagram
MgATP2- + beta-D-fructose 6-phosphate
?
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + beta-D-fructose 6-phosphate
ADP + beta-D-fructose 2,6-bisphosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
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-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphate
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-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
citrate
phosphoenolpyruvate
sn-glycerol 3-phosphate
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Insulin
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activation of transfected heart enzyme in human embryonic kidney 293 cells by inducing the phosphorylation of Ser466 and Ser483, activation is mediated by 3-phosphoinositide-dependent kinase-1, protein kinase B is in vivo not essential, activation is sensitive to LY294002 and wortmannin and insensitive to rapaycin and PD98059
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.055 - 0.26
ATP
0.023 - 0.07
beta-D-fructose 6-phosphate
0.0095 - 0.012
MgATP2-
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pH 7.5, 30°C
additional information
additional information
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comparison of Km of wild-type and mutant enzyme
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
comparison of kcat/Km values of wild-type and mutant enzymes
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
phosphoenolpyruvate
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pH 7.5, 30°C, heart
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
-
0.042
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-
0.075
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-
0.092
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-
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
of 54000 Da and 58000 Da isozymes
6.6
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8.5
-
bovine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 9.5
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about half-maximal activity at pH 7.3 and 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isoform PFKFB2, bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, EC 2.7.1.105 and EC 3.1.3.46, respectively
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cortex
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
F262_BOVIN
531
0
60811
Swiss-Prot
other Location (Reliability: 2)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102000
-
gel filtration
118000
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gel filtration
120000
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2 * 120000, SDS-PAGE
225000
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gel filtration
49000
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2 * 49000, SDS-PAGE
52000
-
2 * 52000, SDS-PAGE
61000
-
x * 61000, SDS-PAGE
97000
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gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 61000, SDS-PAGE
dimer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to 1.8 A resolution. Citrate cocrystallizes in the 2-kinase domain, occupying the fructose-6-phosphate binding-site and extending into the gamma-phosphate binding pocket of ATP. In complex with ADP, ADP mimicks the catalytic binding mode of ATP
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S466E
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mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme in vitro
S466E/S483E
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the double mutant is not phosphorylated by protein kinase B, mutation decreases Km of beta-D-fructose 6-phosphate in vitro
S483E
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mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme and mutation decreases citrate inhibition in vitro
additional information
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mutant enzymes of rat testis are constructed, in which its terminal peptides are replaced with those of the liver or the heart enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms
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mutant enzyme
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wild-type and mutant enzyme
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
wild-type enzyme, S466E and S466E/S483E mutants are expressed in Escherichia coli BL21(DE3)pLysE and S483E mutant is expressed in BL21(DE3)pLysS, human embryonic kidney 293 cells are transiently transfected with vectors expressing wild-type heart enzyme
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
El-Maghrabi, M.R.; Correia, J.J.; Heil, P.J.; Pate, T.M.; Cobb, C.E.; Pilkis, S.J.
Tissue distribution, immunoreactivity, and physical properties of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
Proc. Natl. Acad. Sci. USA
83
5005-5009
1986
Bos taurus, Canis lupus familiaris, Rattus norvegicus
Manually annotated by BRENDA team
Sakurai, T.; Johnson, J.H.; Uyeda, K.
Islet fructose 6-phosphate, 2-kinase: fructose 2,6-bisphosphatase: isozymic form, expression, and characterization
Biochem. Biophys. Res. Commun.
218
159-163
1996
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Sakata, J.; Uyeda, K.
Characterization of two isozymic forms of heart fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase
Biochem. Biophys. Res. Commun.
180
470-474
1991
Bos taurus
Manually annotated by BRENDA team
Rider, M.H.; Foret, D.; Hue, L.
Comparison of purified bovine heart and rat liver 6-phosphofructo-2-kinase. Evidence for distinct isoenzymes
Biochem. J.
231
193-196
1985
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Sakata, J.; Uyeda, K.
Bovine heart fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase: complete amino acid sequence and localization of phosphorylation sites
Proc. Natl. Acad. Sci. USA
87
4951-4955
1990
Bos taurus
Manually annotated by BRENDA team
Kountz, P.D.; El-Maghrabi, M.R.; Pilkis, S.J.
Isolation and characterization of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from bovine liver
Arch. Biochem. Biophys.
238
531-543
1985
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Rider, M.H.; Vandamme, J.; Lebeau, E.; Vertommen, D.; Vidal, H.; Rousseau, G.G.; Vanderkerkhove, J.; Hue, L.
The two forms of bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from alternative splicing
Biochem. J.
285
405-411
1992
Bos taurus, Rattus norvegicus
-
Manually annotated by BRENDA team
Kitamura, K.; Uyeda, K.
Purification and characterization of myocardial fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase [published erratum appears in J Biol Chem 1989 Jan 25;264(3):1901]
J. Biol. Chem.
263
9027-9033
1988
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Ventura, F.; Rosa, J.L.; Ambrosio, S.; Pilkis, S.J.; Bartrons, R.
Bovine brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Evidence for a neural-specific isozyme
J. Biol. Chem.
267
17939-17943
1992
Bos taurus
Manually annotated by BRENDA team
Rider, M.H.
Regulation of fructose-2,6-bisphosphate synthesis and breakdown in heart and skeletal muscle
Biochem. Soc. Trans.
15
988-991
1987
Bos taurus, Columba sp., Rattus norvegicus
Manually annotated by BRENDA team
Tominaga, N.; Tsujikawa, T.; Minami, Y.; Wu, R.F.; Watanabe, F.; Sakakibara, R.; Uyeda, K.
Effect of replacement of the amino and the carboxyl termini of rat testis fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase with those of the liver and heart isozymes
Arch. Biochem. Biophys.
347
275-281
1997
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Bertrand, L.; Alessi, D.R.; Deprez, J.; Deak, M.; Viaene, E.; Rider, M.H.; Hue, L.
Heart 6-phosphofructo-2-kinase activation by insulin results from Ser-466 and Ser-483 phosphorylation and requires 3-phosphoinositide-dependent kinase-1, but not protein kinase B
J. Biol. Chem.
274
30927-30933
1999
Bos taurus
Manually annotated by BRENDA team
Crochet, R.; Kim, J.; Lee, H.; Yim, Y.; Kim, S.; Neau, D.; Lee, Y.
Crystal structure of heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) and the inhibitory influence of citrate on substrate binding
Proteins
85
117-124
2017
Homo sapiens (O60825), Bos taurus (P26285)
Manually annotated by BRENDA team