Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.1.1 - hexokinase and Organism(s) Nicotiana tabacum and UniProt Accession Q6Q8A5

for references in articles please use BRENDA:EC2.7.1.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Nicotiana tabacum
UNIPROT: Q6Q8A5
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Nicotiana tabacum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hexokinase, hexokinase ii, hexokinase 2, hexokinase i, hk ii, hxk, liver glucokinase, hexokinase 1, hexokinase-2, hkdc1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP-D-hexose 6-phosphotransferase
-
-
-
-
ATP-dependent hexokinase
-
-
-
-
brain form hexokinase
-
-
-
-
glucokinase
-
-
-
-
glucose ATP phosphotransferase
-
-
-
-
hexokinase (phosphorylating)
-
-
-
-
hexokinase 1
-
-
hexokinase D
-
-
-
-
hexokinase PI
-
-
-
-
hexokinase PII
-
-
-
-
hexokinase type IV
-
-
-
-
hexokinase type IV glucokinase
-
-
-
-
hexokinase, tumor isozyme
-
-
-
-
HK
-
-
-
-
HK4
-
-
-
-
HXK
-
-
-
-
kinase, hexo- (phosphorylating)
-
-
-
-
muscle form hexokinase
-
-
-
-
additional information
enzyme is a type A hexokinase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-hexose 6-phosphotransferase
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-51-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
isozyme hexokinase 2 plays a role in starch and secondary metabolism in starch sheath, xylem parenchyma, guard cells, and root tips
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
increased activity of the cytosolic and non-cytosolic isozymes induced by tobacco mosaic virus TMV
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
D-fructose
pH 8.4, 30°C, recombinant isozyme hexokinase 2
0.06
D-glucose
pH 8.4, 30°C, recombinant isozyme hexokinase 2
0.149
D-mannose
pH 8.4, 30°C, recombinant isozyme hexokinase 2
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.114
-
healthy leaves, soluble glucokinase activity
0.199
-
TMV-infected leaves, soluble glucokinase activity
0.574
-
healthy leaves, particulate glucokinase activity
0.609
-
healthy leaves, soluble fructokinase activity
0.688
0.693
-
TMV-infected leaves, particulate glucokinase activity
0.892
-
TMV-infected leaves, total glucokinase activity
1.297
-
healthy leaves, total fructokinase activity
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
8.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozyme hexokinase 2
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
vascular starch sheath
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
hexokinase 2, enzyme contains a putative cleavable chloroplast transit peptide of 30 amino acids
Manually annotated by BRENDA team
-
2 soluble isozymes, increased activity of the cytosolic and non-cytosolic isozymes induced by tobacco mosaic virus infection
Manually annotated by BRENDA team
-
1 particulate isozyme, increased activity of the cytosolic and non-cytosolic isozymes induced by tobacco mosaic virus infection
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HXK2_TOBAC
499
0
53997
Swiss-Prot
Chloroplast (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
x * 54000, about, amino acid sequence calculation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 54000, about, amino acid sequence calculation
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partially, subcellular fractionation by anion exchange chromatography of isozymes from healthy and TMV-infected leaves
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
isozyme hexokinase 2, promotor isolation and analysis, in vitro transcription and translation, and import of intact precursor proteins, native or fused to GFP, into stroma of chloroplasts of spinach or pea, expression in transgenic tobacco plants via Agrobacterium tumefaciens infection system
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sindelar, L.; Sindelarova, M.
Hexokinases of tobacco leaves: Changes in the cytosolic and non-cytosolic isozyme complexes induced by tobacco mosaic virus infection
Biol. Plant.
47
413-419
2003
Nicotiana tabacum
-
Manually annotated by BRENDA team
Giese, J.O.; Herbers, K.; Hoffmann, M.; Klosgen, R.B.; Sonnewald, U.
Isolation and functional characterization of a novel plastidic hexokinase from Nicotiana tabacum
FEBS Lett.
579
827-831
2005
Nicotiana tabacum (Q6Q8A5), Nicotiana tabacum
Manually annotated by BRENDA team
Kim, Y.; Heinzel, N.; Giese, J.; Koeber, J.; Melzer, M.; Rutten, T.; Von Wiren, N.; Sonnewald, U.; Hajirezaei, M.
A dual role of tobacco hexokinase 1 in primary metabolism and sugar sensing
Plant Cell Environ.
36
1311-1327
2013
Nicotiana tabacum
Manually annotated by BRENDA team