D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-hexose 6-phosphotransferase
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
compound is nearly 2fold less toxic Plasmodium falciparum lines overexpressing hexokinase compared with control parasites. Although catalytic activity is higher in in overexpressing cells, they accumulate phospho-2-deoxy-D-glucose at the same rate as control parasites
Plasmodium falciparum hexokinase is associated with the membrane via its C-terminal hydrophobic sequence, and this membrane association improves the efficiency of glucose phosphorylation immediately upon entering the host cell. Plasmodium hexokinase ensures a dramatic increase in reduced glutathione production in infected erythrocytes
given the absence of energy stores in this parasite and the key role of the Plasmodium falciparum hexose transporter, the pathway involving Plasmodium falciparum hexokinase and the bifunctional glucose-6-phosphate dehydrogenase 6-phosphogluconolactonase is promising as a pharmacotherapeutic target, particularly because Plasmodium falciparum hexokinase shows a low sequence identity with human hexokinases
Plasmodium falciparum hexokinase activity is important for the NADPH-dependent reduction of oxidized glutathione in the parasite by providing glucose-6-phosphate, which allows glucose-6-phosphate dehydrogenase to generate NADPH for downstream redox reactions
high-throughput assay for screening small molecule collections to identify inhibitors of the Plasmodium falciparum hexokinase. The assay employs an ADP-GloTM reporter system in a 1536-well plate format, is robust with a signal-to-background of 3.4, a percent coefficient of variation of 6.8 and a Z'-factor of 0.75