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Information on EC 2.7.1.1 - hexokinase and Organism(s) Schizosaccharomyces pombe and UniProt Accession P50521

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EC Tree
IUBMB Comments
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: P50521
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The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hexokinase, hexokinase ii, hexokinase 2, hexokinase i, hk ii, hxk, liver glucokinase, hexokinase 1, hexokinase-2, hkdc1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP-D-hexose 6-phosphotransferase
-
-
-
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ATP-dependent hexokinase
-
-
-
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brain form hexokinase
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-
-
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glucokinase
-
-
-
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glucose ATP phosphotransferase
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-
-
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hexokinase (phosphorylating)
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-
-
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hexokinase D
-
-
-
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hexokinase PI
-
-
-
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hexokinase PII
-
-
-
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hexokinase type IV
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-
-
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hexokinase type IV glucokinase
-
-
-
-
hexokinase, tumor isozyme
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-
-
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HK
-
-
-
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HK4
-
-
-
-
HXK
-
-
-
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kinase, hexo- (phosphorylating)
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-
-
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muscle form hexokinase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-hexose = ADP + D-hexose 6-phosphate
show the reaction diagram
equilibrium random Bi Bi mechanism with formation of the abortive enzyme-ADP-glucose complex
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-hexose 6-phosphotransferase
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-51-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-fructose + ATP
ADP + D-fructose 6-phosphate
show the reaction diagram
higher activity than with glucose or mannose
-
-
?
D-glucose + ATP
ADP + D-glucose 6-phosphate
show the reaction diagram
59% of the activity with fructose
-
-
?
D-mannose + ATP
ADP + D-mannose 6-phosphate
show the reaction diagram
42% of the activity with fructose
-
-
?
D-fructose + ATP
ADP + D-fructose 6-phosphate
show the reaction diagram
higher activity than with glucose or mannose
-
-
?
D-glucose + ATP
ADP + D-glucose 6-phosphate
show the reaction diagram
D-glucose + ATP
D-glucose 6-phosphate + ADP
show the reaction diagram
-
enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway
-
?
D-mannose + ATP
ADP + D-mannose 6-phosphate
show the reaction diagram
23% of the activity with fructose
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucose + ATP
D-glucose 6-phosphate + ADP
show the reaction diagram
-
enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
noncompetitive to glucose, competitive to ATP
Mg2+
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at 10 mM, slight
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
2-deoxy-D-glucose
fructose as substrate
2.1
D-glucosamine
fructose as substrate
4
2-deoxy-D-glucose
fructose as substrate
11
D-glucosamine
fructose as substrate
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20.25
-
at pH 7.2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
hexokinase 2
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47500
-
2 * 47500, SDS-PAGE
88000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 50000, calculated from the amino acid sequence
dimer
-
2 * 47500, SDS-PAGE
monomer
1 * 53000, calculated from the amino acid sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
253fold, major isoenzyme form
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
genes hxk1+ and hxk2+, encoding hexokinases 1 and 2, are cloned, sequenced and expressed in Saccharomyces cerevisiae WF8-2C
genes hxk1+ and hxk2+, encoding hexokinases 1 and 2, are cloned, sequenced and expressed in Saccharomyces cerevisiae WF8-2C
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tsai, C.S.; Chen, Q.
Purification and kinetic characterization of hexokinase and glucose-6-phosphate dehydrogenase from Schizosaccharomyces pombe
Biochem. Cell Biol.
76
107-113
1998
Schizosaccharomyces pombe, Schizosaccharomyces pombe NCYC 132S2-2
Manually annotated by BRENDA team
Petit, T.; Blazquez, M.A.; Gancedo, C.
Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: Biochemical and molecular characterization of both hexokinases
FEBS Lett.
378
185-189
1996
Schizosaccharomyces pombe (P50521), Schizosaccharomyces pombe (Q09756), Schizosaccharomyces pombe, Schizosaccharomyces pombe PB003 (Q09756), Schizosaccharomyces pombe PB004 (P50521)
Manually annotated by BRENDA team