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Information on EC 2.7.1.1 - hexokinase and Organism(s) Brugia malayi and UniProt Accession A0A0K0JFP3

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EC Tree
IUBMB Comments
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
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This record set is specific for:
Brugia malayi
UNIPROT: A0A0K0JFP3
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Word Map
The taxonomic range for the selected organisms is: Brugia malayi
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hexokinase, hexokinase ii, hexokinase 2, hexokinase i, hk ii, hxk, liver glucokinase, hexokinase 1, hexokinase-2, hkdc1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
BmHk
Q56VN6
-
ATP-D-hexose 6-phosphotransferase
-
-
-
-
ATP-dependent hexokinase
-
-
-
-
brain form hexokinase
-
-
-
-
glucokinase
-
-
-
-
glucose ATP phosphotransferase
-
-
-
-
hexokinase (phosphorylating)
-
-
-
-
hexokinase D
-
-
-
-
hexokinase PI
-
-
-
-
hexokinase PII
-
-
-
-
hexokinase type IV
-
-
-
-
hexokinase type IV glucokinase
-
-
-
-
hexokinase, tumor isozyme
-
-
-
-
HK
-
-
-
-
HK4
-
-
-
-
HXK
-
-
-
-
kinase, hexo- (phosphorylating)
-
-
-
-
muscle form hexokinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-hexose 6-phosphotransferase
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-51-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q56VN6
-
-
-
?
ATP + D-galactose
?
show the reaction diagram
Q56VN6
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
Q56VN6
-
-
-
?
ATP + D-maltose
?
show the reaction diagram
Q56VN6
-
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
Q56VN6
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
Q56VN6
20 mM, 96% inhibition
ADP
Q56VN6
0.0001 mM, 50% inhibition. Noncompetitive inhibition with the substrate glucose
CaCl2
Q56VN6
10 mM, 98% inhibition
D-glucosamine
Q56VN6
-
D-glucose 6-phosphate
Q56VN6
-
diphosphate
Q56VN6
mixed type of inhibition when ATP is used as substrate
EDTA
Q56VN6
40 mM, 91% inhibition
KCl
Q56VN6
10 mM, 92% inhibition
mannoheptulose
Q56VN6
-
Mg2+
Q56VN6
100 mM, 72% inhibition
N-acetyl glucosamine
Q56VN6
-
NaCl
Q56VN6
10 mM, 96% inhibition
p-chloromercuribenzoate
Q56VN6
0.05 mM, 72% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glucose-6-phosphate
Q56VN6
10 mM, recombinant enzyme is 3fold activated
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.09
ATP
Q56VN6
-
75
D-fructose
Q56VN6
-
0.035
D-glucose
Q56VN6
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
ADP
Q56VN6
substrate glucose
0.009
diphosphate
Q56VN6
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.4
Q56VN6
-
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HXK_BRUMA
572
0
64043
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72000
Q56VN6
4 * 72000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Q56VN6
4 * 72000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
Q56VN6
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
Q56VN6
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Singh, A.R.; Joshi, S.; Arya, R.; Kayastha, A.M.; Srivastava, K.K.; Tripathi, L.M.; Saxena, J.K.
Molecular cloning and characterization of Brugia malayi hexokinase
Parasitol. Int.
57
354-361
2008
Brugia malayi (Q56VN6), Brugia malayi
Manually annotated by BRENDA team