Information on EC 2.6.1.95 - neomycin C transaminase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.6.1.95
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RECOMMENDED NAME
GeneOntology No.
neomycin C transaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
neomycin C + 2-oxoglutarate = 6'''-deamino-6'''-oxoneomycin C + L-glutamate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
neomycin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
2-oxoglutarate:neomycin C aminotransferase
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 1.1.3.44, 6'''-hydroxyneomycin C oxidase, to replace the 6'''-hydroxy group of 6'''-deamino-6'''-hydroxyneomycin C with an amino group. The enzyme, characterized from the bacterium Streptomyces fradiae, can also catalyse EC 2.6.1.93, neamine transaminase.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
neomycin + 2-oxoglutarate
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
show the reaction diagram
neomycin C + 2-oxoglutarate
6'''-hydroxyneomycin C + L-glutamate
show the reaction diagram
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?
additional information
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NeoB is a bifunctional enzyme that also catalyses the NH2 group transfer at the 6'''-position
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48060
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Neo-18, calculated from amino acid sequence; Neo-18, electrospray ionization mass spectrometry
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA resin column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
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LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.95)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)