Information on EC 2.6.1.80 - nicotianamine aminotransferase

for references in articles please use BRENDA:EC2.6.1.80
Word Map on EC 2.6.1.80
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
2.6.1.80
-
top print hide
RECOMMENDED NAME
GeneOntology No.
nicotianamine aminotransferase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nicotianamine + 2-oxoglutarate = 3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2'-deoxymugineic acid phytosiderophore biosynthesis
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
nicotianamine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. This enzyme is produced by grasses. They secrete both the nicotianamine and the transaminated product into the soil around them. Both compounds chelate iron(II) and iron(III); these chelators, called mugineic acid family phytosiderophores, are taken up by the grass, which is thereby supplied with iron.
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nicotianamine + 2-oxoglutarate
3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
additional information
?
-
-
a point mutation in a gene encoding nicotianamine aminotransferase (NAAT1) stimulates the Fe(II) acquisition system and leads to iron accumulation in rice
-
-
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
nicotianamine + 2-oxoglutarate
3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
additional information
?
-
-
a point mutation in a gene encoding nicotianamine aminotransferase (NAAT1) stimulates the Fe(II) acquisition system and leads to iron accumulation in rice
-
-
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 0.9
nicotianamine
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0005
-
-
0.00088
-
-
top print hide
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
-
deduced from nucleotide sequence
6.8
-
deduced from nucleotide sequence
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
OsNAAT1 is induced in all the cells of Fe-deficient leaves
Manually annotated by BRENDA team
OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots. OsNAAT1 expression is observed in the companion cells of Fe-sufficient shoots
Manually annotated by BRENDA team
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46600
-
x * 46600, deduced from nucleotide sequence; x * 46600, deduced from nucleotide sequence
80000
-
gel filtration
90000
-
gel filtration
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 46600, deduced from nucleotide sequence; x * 46600, deduced from nucleotide sequence
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Butyl Toyopearl 650M, hydroxylapatite, DEAE-Sephacel
-
Butyl toyopearl, hydroxylapatite, DEAE-Sepharose; Butyl toyopearl, hydroxylapatite, DEAE-Sepharose; Butyl toyopearl, hydroxylapatite, DEAE-Sepharose, NA-Sepharose
-
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Oryza sativa; expression in Oryza sativa
-
expression in Saccharomyces cerevisiae; expression in Saccharomyces cerevisiae
-
top print hide
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
production of biofortified rice via introduction of the soybean ferritin gene (Soyfer H2) driven by two endosperm-specific promoters, along with the barley nicotianamine synthase gene NAS1, two nicotianamine aminotransferase genes Naat-A and Naat-B, and a mugineic acid synthase gene IDS3 to enhance mugineic acid production in rice plants. Lines expressing both ferritin and mugineic acid biosynthetic genes show signs of iron-deficiency tolerance in calcareous soil. The iron concentration in polished T3 seeds is increased by 4- and 2.5times, respectively; production of biofortified rice via introduction of the soybean ferritin gene (Soyfer H2) driven by two endosperm-specific promoters, along with the barley nicotianamine synthase gene NAS1, two nicotianamine aminotransferase genes Naat-A and Naat-B, and a mugineic acid synthase gene IDS3 to enhance mugineic acid production in rice plants. Lines expressing both ferritin and mugineic acid biosynthetic genes show signs of iron-deficiency tolerance in calcareous soil. The iron concentration in polished T3 seeds is increased by 4- and 2.5times, respectively
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.80)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)