Information on EC 2.6.1.80 - nicotianamine aminotransferase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.6.1.80
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RECOMMENDED NAME
GeneOntology No.
nicotianamine aminotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nicotianamine + 2-oxoglutarate = 3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2'-deoxymugineic acid phytosiderophore biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
nicotianamine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. This enzyme is produced by grasses. They secrete both the nicotianamine and the transaminated product into the soil around them. Both compounds chelate iron(II) and iron(III); these chelators, called mugineic acid family phytosiderophores, are taken up by the grass, which is thereby supplied with iron.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nicotianamine + 2-oxoglutarate
3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
additional information
?
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a point mutation in a gene encoding nicotianamine aminotransferase (NAAT1) stimulates the Fe(II) acquisition system and leads to iron accumulation in rice
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
nicotianamine + 2-oxoglutarate
3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
additional information
?
-
-
a point mutation in a gene encoding nicotianamine aminotransferase (NAAT1) stimulates the Fe(II) acquisition system and leads to iron accumulation in rice
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-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 0.9
nicotianamine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0005
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-
0.00088
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-
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
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deduced from nucleotide sequence
6.8
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deduced from nucleotide sequence
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
OsNAAT1 is induced in all the cells of Fe-deficient leaves
Manually annotated by BRENDA team
OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots. OsNAAT1 expression is observed in the companion cells of Fe-sufficient shoots
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46600
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x * 46600, deduced from nucleotide sequence; x * 46600, deduced from nucleotide sequence
80000
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gel filtration
90000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 46600, deduced from nucleotide sequence; x * 46600, deduced from nucleotide sequence
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Butyl Toyopearl 650M, hydroxylapatite, DEAE-Sephacel
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Butyl toyopearl, hydroxylapatite, DEAE-Sepharose; Butyl toyopearl, hydroxylapatite, DEAE-Sepharose; Butyl toyopearl, hydroxylapatite, DEAE-Sepharose, NA-Sepharose
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Oryza sativa; expression in Oryza sativa
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expression in Saccharomyces cerevisiae; expression in Saccharomyces cerevisiae
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
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production of biofortified rice via introduction of the soybean ferritin gene (Soyfer H2) driven by two endosperm-specific promoters, along with the barley nicotianamine synthase gene NAS1, two nicotianamine aminotransferase genes Naat-A and Naat-B, and a mugineic acid synthase gene IDS3 to enhance mugineic acid production in rice plants. Lines expressing both ferritin and mugineic acid biosynthetic genes show signs of iron-deficiency tolerance in calcareous soil. The iron concentration in polished T3 seeds is increased by 4- and 2.5times, respectively; production of biofortified rice via introduction of the soybean ferritin gene (Soyfer H2) driven by two endosperm-specific promoters, along with the barley nicotianamine synthase gene NAS1, two nicotianamine aminotransferase genes Naat-A and Naat-B, and a mugineic acid synthase gene IDS3 to enhance mugineic acid production in rice plants. Lines expressing both ferritin and mugineic acid biosynthetic genes show signs of iron-deficiency tolerance in calcareous soil. The iron concentration in polished T3 seeds is increased by 4- and 2.5times, respectively
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LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.80)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)