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Information on EC 2.6.1.42 - branched-chain-amino-acid transaminase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LE06

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EC Tree
     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.42 branched-chain-amino-acid transaminase
IUBMB Comments
Also acts on L-isoleucine and L-valine, and thereby differs from EC 2.6.1.6, leucine transaminase, which does not. It also differs from EC 2.6.1.66, valine---pyruvate transaminase.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9LE06
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
bcat1, bcatm, branched-chain aminotransferase, bcatc, bcat2, branched-chain amino acid aminotransferase, branched chain aminotransferase, hbcat, hbcatm, branched-chain amino acid transaminase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
At1g50110
locus name
branched-chain amino acid aminotransferase
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branched-chain amino acid-glutamate transaminase
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branched-chain aminotransferase
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branched-chain aminotransferase4
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glutamate-branched-chain amino acid transaminase
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L-branched chain amino acid aminotransferase
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transaminase B
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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SYSTEMATIC NAME
IUBMB Comments
branched-chain-amino-acid:2-oxoglutarate aminotransferase
Also acts on L-isoleucine and L-valine, and thereby differs from EC 2.6.1.6, leucine transaminase, which does not. It also differs from EC 2.6.1.66, valine---pyruvate transaminase.
CAS REGISTRY NUMBER
COMMENTARY hide
9054-65-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate + L-isoleucine
L-glutamate + 3-methyl-2-oxopentanoate
show the reaction diagram
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-
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r
2-oxoglutarate + L-leucine
L-glutamate + 4-methyl-2-oxopentanoate
show the reaction diagram
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-
-
r
3-methyl-2-oxobutanoate + L-glutamate
L-valine + 2-oxoglutarate
show the reaction diagram
-
-
-
r
4-methylthio-2-oxobutanoate + L-glutamate
L-methionine + 2-oxoglutarate
show the reaction diagram
-
-
-
r
L-2-aminobutanoate + 4-methylthio-2-oxo-butanoate
2-oxobutanoate + L-methionine
show the reaction diagram
-
-
-
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r
L-glutamate + 3-methyl-2-oxopentanoate
2-oxoglutarate + L-isoleucine
show the reaction diagram
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-
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r
L-glutamate + 4-methyl-2-oxopentanoate
2-oxoglutarate + L-leucine
show the reaction diagram
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-
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r
L-homomethionine + 2-oxoglutarate
5-methylthio-2-oxopentanoate + L-glutamate
show the reaction diagram
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-
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-
?
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
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-
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-
?
L-methionine + 2-oxobutanoate
4-methylthio-2-oxobutanoate + L-2-aminobutanoate
show the reaction diagram
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-
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r
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + L-glutamate
show the reaction diagram
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?
L-methionine + 2-oxoglutarate
4-methylthio-2-oxobutanoate + L-glutamate
show the reaction diagram
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r
L-valine + 2-oxoglutarate
3-methyl-2-oxobutanoate + L-glutamate
show the reaction diagram
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r
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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enzyme may influence methionine levels and play an important role in the metabolism of the nonprotein amino acid alpha-aminobutanoate
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.69
3-methyl-2-oxobutanoate
pH 8.3, 337°C
1.08
3-methyl-2-oxopentanoate
pH 8.3, 337°C
2.64
4-methyl-2-oxopentanoate
pH 8.3, 337°C
0.045
4-methylsulfanyl-2-oxobutanoate
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2.83
4-methylthio-2-oxobutanoate
pH 8.3, 337°C
3.27
L-isoleucine
pH 8.3, 337°C
3.34 - 4.86
L-leucine
0.93 - 6.58
L-methionine
4.41
L-valine
pH 8.3, 337°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
AtBCAT-4
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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predominant expression of isoform Atbcat-1
Manually annotated by BRENDA team
additional information
-
isoforms Atbcat-1, Atbcat-3 are expressed in all tissues, isoforms Atbcat-4, Atbcat-6 mainly in tissues associated with transport function and meristematic tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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isoforms Atbcat-4, Atbcat-6
Manually annotated by BRENDA team
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isoform Atbcat-1
Manually annotated by BRENDA team
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isoforms Atbcat-2, Atbcat-3, Atbcat-5
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
mutant plants lacking the activities of isoforms bcat3, Bcat4, Bcat6 exhibit a clear macroscopic phenotype with smaller plants and abnormal leaf morphology. The triple mutant shows a dramatic reduction of Met-derived glucosinolate species down to 32 and 14% of wild-type levels in plant foliage and seeds, respectively, accompanied by a 46fold increase of free Met. 5?-Deoxy-5?-methylthioadenosine, an intermediate of the Met recycling pathway, accumulates to relative high amounts in the absence of the cytosolic Bcat4 and Bcat6
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BCAT4_ARATH
354
0
39019
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51900
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predicted mass
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
S-protein agarose chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Diebold, R.; Schuster, J.; Daeschner, K.; Binder, S.
The branched-chain amino acid transaminase gene family in Arabidopsis encodes plastid and mitochondrial proteins
Plant Physiol.
129
540-550
2002
Arabidopsis sp., Arabidopsis thaliana (Q93Y32), Arabidopsis thaliana (Q9FYA6), Arabidopsis thaliana (Q9LE06), Arabidopsis thaliana (Q9LPM9), Arabidopsis thaliana (Q9M401), Arabidopsis thaliana (Q9M439)
Manually annotated by BRENDA team
Schuster, J.; Binder, S.
The mitochondrial branched-chain aminotransferase (AtBCAT-1) is capable to initiate degradation of leucine, isoleucine and valine in almost all tissues in Arabidopsis thaliana
Plant Mol. Biol.
57
241-254
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Schuster, J.; Knill, T.; Reichelt, M.; Gershenzon, J.; Binder, S.
Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis
Plant Cell
18
2664-2679
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Laechler, K.; Imhof, J.; Reichelt, M.; Gershenzon, J.; Binder, S.
The cytosolic branched-chain aminotransferases of Arabidopsis thaliana influence methionine supply, salvage and glucosinolate metabolism
Plant Mol. Biol.
88
119-131
2015
Arabidopsis thaliana (Q9LPM9), Arabidopsis thaliana
Manually annotated by BRENDA team