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Information on EC 2.6.1.4 - glycine transaminase and Organism(s) Homo sapiens and UniProt Accession Q9Y617

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EC Tree
     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.4 glycine transaminase
IUBMB Comments
A pyridoxal-phosphate protein.
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This record set is specific for:
Homo sapiens
UNIPROT: Q9Y617
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
psat1, glutamate:glyoxylate aminotransferase, glyoxylate-glutamate aminotransferase, glycine transaminase, l-glutamate:glyoxylate aminotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminotransferase, glycine
-
-
-
-
glutamate-glycine transaminase
-
-
glutamic-glyoxylic transaminase
-
-
-
-
glycine aminotransferase
-
-
-
-
glyoxylate-glutamate aminotransferase
-
-
-
-
glyoxylate-glutamic transaminase
-
-
-
-
L-glutamate:glyoxylate aminotransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-99-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-alanine + glyoxylate
pyruvate + glycine
show the reaction diagram
-
-
-
?
L-asparagine + glyoxylate
4-amino-2,4-dioxobutanoate + glycine
show the reaction diagram
-
-
-
?
L-glutamate + glyoxylate
2-oxoglutarate + glycine
show the reaction diagram
L-Glu is the preferred amino-group donor both at 25°C and at 37°C
-
-
?
L-homocysteate + glyoxylate
? + glycine
show the reaction diagram
-
-
-
?
L-serine + glyoxylate
3-hydroxy-2-oxopropanoate + glycine
show the reaction diagram
-
-
-
?
glyoxylate + L-alanine
glycine + pyruvate
show the reaction diagram
-
-
-
-
ir
glyoxylate + L-glutamate
glycine + 2-oxoglutarate
show the reaction diagram
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
-
-
-
-
ir
L-alanine + 2-oxoglutarate
pyruvate + L-glutamate
show the reaction diagram
-
-
-
?
L-alanine + glyoxylate
pyruvate + glycine
show the reaction diagram
L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate
-
-
?
L-glutamate + glyoxylate
2-oxoglutarate + glycine
show the reaction diagram
L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glyoxylate + L-glutamate
glycine + 2-oxoglutarate
show the reaction diagram
-
may be involved in control of human oxalate synthesis by providing alternate pathway for glyoxylate metabolism
-
ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
pyridoxal 5'-phosphate
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
strong
hydroxylamine
-
no inhibition
Isonicotinic acid hydrazide
-
no inhibition
Maleic acid
-
no inhibition
PCMB
-
-
phenylhydrazine
-
pyridoxal phosphate restores activity
additional information
-
no inhibition by CN-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.5
glyoxylate
pH 7.4, 37°C, cosubstrate: L-glutamate
2
glutamate
-
pH 7.7, 37°C
0.75 - 2
glyoxylate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5
pH 7.4, 25°C, L-glutamate (1 mM) + glyoxylate
0.185
-
purified enzyme
2.1
pH 7.4, 25°C, L-alanine (10 mM) + glyoxylate
2.5
pH 7.4, 25°C, L-glutamate (10 mM) + glyoxylate
87
pH 7.5, 25°C, L-alanine + 2-oxoglutarate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 8.5
-
about half-maximal activity at pH 6.3 and about 60% of maximal activity at pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SERC_HUMAN
370
0
40423
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
recombinant enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
290fold
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpressed as His6-tagged protein in Escherichia coli cells
overexpressed as His6-tagged protein in Escherichia coli cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Thompson, J.S.; Richardson, K.E.
Isolation and characterization of a glutamate-glycine transaminase from human liver
Arch. Biochem. Biophys.
117
599-603
1966
Homo sapiens
-
Manually annotated by BRENDA team
Donini, S.; Ferrari, M.; Fedeli, C.; Faini, M.; Lamberto, I.; Marletta, A.S.; Mellini, L.; Panini, M.; Percudani, R.; Pollegioni, L.; Caldinelli, L.; Petrucco, S.; Peracchi, A.
Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism
Biochem. J.
422
265-272
2009
Homo sapiens (P24298), Homo sapiens (Q9Y617), Homo sapiens
Manually annotated by BRENDA team