We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.6.1.114 - 8-demethyl-8-aminoriboflavin-5'-phosphate synthase for references in articles please use BRENDA:EC2.6.1.114
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme, characterized from the bacterium Streptomyces davawensis, has the activities of an oxidoreductase, a decarboxylase, and an aminotransferase. Its combined actions result in the replacement of a methyl substituent of one of the aromatic rings of FMN by an amino group, a step in the biosynthetic pathway of roseoflavin. The reaction requires thiamine for completion.
The expected taxonomic range for this enzyme is: Streptomyces davaonensis
Synonyms
8-amino-flavin synthase, 8-demethyl-8-aminoriboflavin-5’-phosphate synthase, AFP synthase,
BN159_7989 ,
rosB ,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8-amino-flavin synthase
-
8-demethyl-8-aminoriboflavin-5’-phosphate synthase
-
-
BN159_7989
-
-
rosB
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor = 8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
(1b)
-
-
-
FMN + O2 = 8-demethyl-8-formylriboflavin 5'-phosphate + H2O
(1a)
-
-
-
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + 2 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 2 reduced acceptor
(1c)
-
-
-
L-glutamate + FMN + O2 + H2O + 3 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 3 reduced acceptor
overall reaction
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-glutamate:FMN aminotransferase (oxidizing, decarboxylating)
The enzyme, characterized from the bacterium Streptomyces davawensis, has the activities of an oxidoreductase, a decarboxylase, and an aminotransferase. Its combined actions result in the replacement of a methyl substituent of one of the aromatic rings of FMN by an amino group, a step in the biosynthetic pathway of roseoflavin. The reaction requires thiamine for completion.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
-
-
-
-
?
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
-
-
-
?
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
-
-
-
-
?
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
-
-
-
?
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
-
?
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
?
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
-
?
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
-
-
-
-
?
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
-
-
-
?
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
-
-
-
-
?
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
-
-
-
?
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
-
?
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
?
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
-
?
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ROSB_STRDJ
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
257
0
28865
Swiss-Prot
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
homotetramer
-
4 * 29000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ni-NTA column chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Schwarz, J.; Konjik, V.; Jankowitsch, F.; Sandhoff, R.; Mack, M.
Identification of the key enzyme of roseoflavin biosynthesis
Angew. Chem. Int. Ed. Engl.
55
6103-6106
2016
Streptomyces davaonensis
brenda
Konjik, V.; Bruenle, S.; Demmer, U.; Vanselow, A.; Sandhoff, R.; Ermler, U.; Mack, M.
The crystal structure of RosB insights into the reaction mechanism of the first member of a family of flavodoxin-like enzymes
Angew. Chem. Int. Ed. Engl.
56
1146-1151
2017
Streptomyces davaonensis
brenda
Jhulki, I.; Chanani, P.; Abdelwahed, S.; Begley, T.
A remarkable oxidative cascade that replaces the riboflavin C8 methyl with an amino group during roseoflavin biosynthesis
J. Am. Chem. Soc.
138
8324-8327
2016
Streptomyces davaonensis (K4REZ6)
brenda
Select items on the left to see more content.
html completed