A pyridoxal-phosphate protein. Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. Aspartate transaminase activity can be formed from the aromatic-amino-acid transaminase (EC 2.6.1.57) of Escherichia coli by controlled proteolysis , some EC 2.6.1.57 activity can be found in this enzyme from other sources ; indeed the enzymes are identical in Trichomonas vaginalis .
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SYSTEMATIC NAME
IUBMB Comments
L-aspartate:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. Aspartate transaminase activity can be formed from the aromatic-amino-acid transaminase (EC 2.6.1.57) of Escherichia coli by controlled proteolysis [7], some EC 2.6.1.57 activity can be found in this enzyme from other sources [8]; indeed the enzymes are identical in Trichomonas vaginalis [6].
anti-enzyme antibodies selectively inhibit the uptake of oleate in 3T3-L1 adipocytes by 31-55%, but only poorly in fibroblasts, the antibodies have no effect on uptake of 2-deoxyglucose and octanoate
hyperprolinemia type II is an autosomal recessive disorder caused by severe deficiency of enzyme DELTA1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Proline has direct inhibitory effect on aspartate transaminase activity of different brain regions leading to lesser synthesis of glutamate thereby causing neurological dysfunctions
anti-enzyme antibodies selectively inhibit the uptake of oleate in 3T3-L1 adipocytes by 31-55%, but only poorly in fibroblasts, the antibodies have no effect on uptake of 2-deoxyglucose and octanoate
hyperprolinemia type II is an autosomal recessive disorder caused by severe deficiency of enzyme DELTA1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Proline has direct inhibitory effect on aspartate transaminase activity of different brain regions leading to lesser synthesis of glutamate thereby causing neurological dysfunctions
inhibitor of C-S lyase, reduces renal injuries due to cisplatin in rats. On day 5 following a bolus cisplatin injection, in vivo nephrotoxic potentials are in the decreasing order of species rats > mice, rabbits, based on body surface. The levels of renal Pt residue at the nephrotoxic dose are in order of rabbits > rats > mice. The activity of endogenous basal mitochondrial aspartate aminotransferase, one of the C-S lyases, in the renal cortex of naive animals is rats > mice, rabbits. Expression of mitochondrial C-S lyase in the kidney is observed at approximately 37 kDa in all five species used. In in vitro studies, the cytotoxicity of cisplatin is dependent on the expression level of C-S lyase mRNA in the respective renal cells
hyperprolinemia type II is an autosomal recessive disorder caused by severe deficiency of enzyme DELTA1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Proline has direct inhibitory effect on aspartate transaminase activity of different brain regions leading to lesser synthesis of glutamate thereby causing neurological dysfunctions
cobalt nanoparticles with particle size less than 50 nm significantly activate the enzyme in the serum, liver, and kidney of rats at concentration-dependent order with a maximum activation of 175% at 10 mM
intramitochondrial chaperone homologues GroEL and GroES can facilitate the folding of nascent premature mitochondrial isoform pmAspAT in rabbit reticulocyte lysate under conditions where it otherwise would not, GroEL alone inhibits the import into mitochondria, which is reversed by GroES
cytosolic and mitochondrial isozymes bind differently to the 70 kDa heat shock protein Hsp70, which regulates the folding and aggregation process of polypeptides, sequence and distribution of Hsp70-binding regions allow the classification of isozymes into a phylogenetic tree, overview
cytosolic and mitochondrial isozymes bind differently to the 70 kDa heat shock protein Hsp70, which regulates the folding and aggregation process of polypeptides, sequence and distribution of Hsp70-binding regions allow the classification of isozymes into a phylogenetic tree, overview
cytosolic and mitochondrial isozymes bind differently to the 70 kDa heat shock protein Hsp70, which regulates the folding and aggregation process of polypeptides, sequence and distribution of Hsp70-binding regions allow the classification of isozymes into a phylogenetic tree, overview
cytosolic and mitochondrial isozymes bind differently to the 70 kDa heat shock protein Hsp70, which regulates the folding and aggregation process of polypeptides, sequence and distribution of Hsp70-binding regions allow the classification of isozymes into a phylogenetic tree, overview
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
guanidinium hydrochloride denatured premature form pmAspAT cannot refold at 30°C, but refolds rapidly in presence of the intramitochondrial chaperone homologues GroEL and GroES
unfolding in 6 M guanidine hydrochloride for different periods of time. Reactivation of equilibrium-unfolded mAAT is sigmoidal, reactivation of the short term unfolded protein displays a double exponential behavior consistent with the presence of fast and slow refolding species. The presence of coenzyme does not perturb the kinetics or pathway of refolding. Covalently attached PLP slows down the interconversion between fast and slow folding populations of unfolded states. Additional structural rearrangements occurring both in the unfolded state and in populations of folding intermediates along the folding pathway
the inhibitor of C-S lyase, aminooxyacetic acid hemihydrochloride, reduces renal injuries due to cisplatin in rats. On day 5 following a bolus cisplatin injection, in vivo nephrotoxic potentials are in the decreasing order of species rats > mice, rabbits, based on body surface. The levels of renal Pt residue at the nephrotoxic dose are in order of rabbits > rats > mice. The activity of endogenous basal mitochondrial aspartate aminotransferase, one of the C-S lyases, in the renal cortex of naive animals is rats > mice, rabbits. Expression of mitochondrial C-S lyase in the kidney is observed at approximately 37 kDa in all five species used. In in vitro studies, the cytotoxicity of cisplatin is dependent on the expression level of C-S lyase mRNA in the respective renal cells
Cysteine sulfinate aminotransferase and aspartate aminotransferase isoenzymes of rat brain. Purification, characterization, and further evidence for identity
Artigues, A.; Crawford, D.L.; Iriarte, A.; Martinez-Carrion, M.
Divergent Hsc70 binding properties of mitochondrial and cytosolic aspartate aminotransferase. Implications for their segregation to different cellular compartments
McKenna, M.C.; Hopkins, I.B.; Lindauer, S.L.; Bamford, P.
Aspartate aminotransferase in synaptic and nonsynaptic mitochondria: differential effect of compounds that influence transient hetero-enzyme complex (metabolon) formation