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EC Tree
IUBMB Comments The enzyme is responsible for pyruvylation of the subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium leguminosarum (bv. viciae strain VF39) which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa.
The enzyme appears in viruses and cellular organisms
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phosphoenolpyruvate + [beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2(or 3)-O-Ac-alpha-D-Glc-(1->6)]n = [beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->3)-4,6-CH3(COO-)C-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2(or 3)-O-Ac-alpha-D-Glc-(1->6)]n + phosphate
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phosphoenolpyruvate:[beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-4,6-CH3(COO-)C-beta-D-Gal-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2(or 3)-O-Ac-alpha-D-Glc-(1->6)]n 4,6-O-(1-carboxyethan-1,1-diyl)transferase
The enzyme is responsible for pyruvylation of the subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium leguminosarum (bv. viciae strain VF39) which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa.
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phosphoenolpyruvate + [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n
[D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->3)-4,6-CH3(COO-)C-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n + phosphate
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phosphoenolpyruvate + [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n
[D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->3)-4,6-CH3(COO-)C-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n + phosphate
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UniProt
brenda
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malfunction
knock-out of pssM leads to the absence of the pyruvic acid ketal group at the subterminal glucose in the repeating unit of exopolysaccharide (shown by 13C and 1H nuclear magnetic resonance analysis)
physiological function
Rhizobium leguminosarum bv. viciae strain VF39 synthesizes an acidic exopolysaccharide, which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa. This exopolysaccharide is a polymer of an octasaccharide repeating unit consisting of a backbone of two glucose and two glucuronic acid residues, a side chain of three glucose and one gaThe pssM gene encodes ketal pyruvate transferase, which is responsible for pyruvylation of subterminal glucose in the EPS repeating unit
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PSSM_RHILV
300
0
34182
Swiss-Prot
-
A0A1R3TGI2_9RHIZ
316
0
35335
TrEMBL
-
A0A644VM22_9ZZZZ
287
0
32386
TrEMBL
other Location (Reliability: 2 )
A0A418SD32_9RHOB
849
0
93801
TrEMBL
-
A0A1Z3HH05_9CYAN
155
0
17730
TrEMBL
-
A0A2S0Q6V3_NODSP
287
0
32927
TrEMBL
-
A0A5B9QAZ3_9BACT
278
0
31207
TrEMBL
-
A0A653T208_9MICO
295
0
31907
TrEMBL
-
A0A1Z4BXK7_9GAMM
288
0
32362
TrEMBL
-
A0A2Z2NR85_9GAMM
308
0
35163
TrEMBL
-
A0A1Y5SK01_9RHOB
296
0
31773
TrEMBL
-
A0A238D222_THIDL
300
0
33769
TrEMBL
-
A0A1J5RGF6_9ZZZZ
300
0
33782
TrEMBL
other Location (Reliability: 2 )
A0A5J4F3P5_MICAE
240
0
27267
TrEMBL
-
A0A085EUA5_9RHIZ
297
0
32449
TrEMBL
-
A0A2R8C955_9RHOB
232
0
24980
TrEMBL
-
A0A2K9Z6W4_RHILE
300
0
34265
TrEMBL
-
A0A2K4XA33_PSEVC
293
0
33133
TrEMBL
-
A0A1C3JC64_9VIBR
278
0
31315
TrEMBL
-
A0A0M6YIN9_9RHOB
343
0
37692
TrEMBL
-
A0A3Q9J1Q1_9MICO
248
0
27295
TrEMBL
-
A0A1X7BTN1_9RHOB
301
0
33065
TrEMBL
-
A0A2S5CMQ0_9GAMM
294
0
33100
TrEMBL
-
A0A1R3TQ32_9RHIZ
316
0
35335
TrEMBL
-
A0A011PFI1_9PROT
266
0
29257
TrEMBL
-
A0A395LDV8_9RHIZ
274
0
30837
TrEMBL
-
A0A5A5RXF9_MICAE
208
0
23170
TrEMBL
-
A0A1Q9R5H6_PSEPU
322
0
35640
TrEMBL
-
A0A518HR15_9BACT
290
0
32231
TrEMBL
-
A0A5P9JRB4_9PSED
263
0
29203
TrEMBL
-
A0A1E7X174_9BURK
301
0
33825
TrEMBL
-
A0A518L3L1_9BACT
284
0
31470
TrEMBL
-
A0A2H6HS49_9BACT
256
1
28955
TrEMBL
-
A0A508SW60_9BRAD
293
0
33442
TrEMBL
-
A0A143Y391_9FIRM
289
0
33235
TrEMBL
-
A0A518BTL4_9BACT
298
0
33969
TrEMBL
-
A0A238JKI2_9RHOB
306
0
34467
TrEMBL
-
A0A7M4DI78_9MICO
302
0
32860
TrEMBL
-
A0A395KX69_9RHIZ
316
0
35402
TrEMBL
-
A0A0F0KZN5_9MICO
295
1
31852
TrEMBL
-
A0A7M1WRH1_VIBPH
276
0
31544
TrEMBL
-
W2UHB8_9GAMM
276
0
30698
TrEMBL
-
A0A1X7A838_9RHOB
267
0
28450
TrEMBL
-
A0A517M0E3_9BACT
217
0
24852
TrEMBL
-
A0A143QPK8_9NOCA
270
0
30213
TrEMBL
-
A0A447ITE0_9RHOB
329
0
36138
TrEMBL
-
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Ivashina, T.V.; Fedorova, E.E.; Ashina, N.P.; Kalinchuk, N.A.; Druzhinina, T.N.; Shashkov, A.S.; Shibaev, V.N.; Ksenzenko, V.N.
Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis
J. Appl. Microbiol.
109
731-742
2010
Rhizobium leguminosarum (Q9FCP2)
brenda
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