compounds showing strong competition/inhibition are simple phenols having phenolic OH residues, while carboxylic acid does not seem to influence the enzyme activity. Phenylpropanoid gives no apparent inhibition. No or poor inhibition by benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, ferulic acid, and 4-coumaric acid
the mRNA of the LePGT-1 gene is undetectable in aerial plant tissues, and is exclusively detected in root tissues, similar to shikonin accumulation; the mRNA of the LePGT-2 gene is undetectable in aerial plant tissues, and is exclusively detected in root tissues, similar to shikonin accumulation
the enzyme is part of a metabolic strategy to further controll the respiratory levels in CoQ8 biosynthesis-deficient Escherichia coli strains, this metabolic strategy can lead to fine-tuning of aerobic lactate accumulation. The lactate and acetate concentrations increase with increasing enzyme levels, as does the cell density, while glucose concentration decreases, overview
the amino acid residues of LePGT1 critical for the enzymatic activity and the region responsible for the binding of the substrates are elucidated by mutational analysis. Substrate specificity is analysed using chimeric enzymes derived from LePGT1 and UbiA (EC 22.214.171.124). In vitro and in vivo analysis of the chimeras suggests that the determinant region for this specificity is within 130 amino acids of the N-terminal
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-20°C, in the presence of 10% glycerol, frozen enzyme preparations are stable upon storage for at least two months. Protease inhibitors directed against serin-, carboxyl-, and metalloproteases such as PMSF, 1,2-epoxy-3-(4-nitrophenoxy)-propane or EGTA do not increase the stability of the enzyme solution.
recombiant expression in Escherichia coli coenzyme Q8 biosynthesis-deficient strains, HW108 and HW109, which contain mutations in ubiE and ubiG, respectively. Gene lePGT-1 gene is amplified and cloned into the expression vector, pBAD33, to form pBlePGT, the fine-tuning of the expression of lePGT-1 in the ubiquinone-deficient strains, HW108 and HW109, to control the electron transfer chain is investigated by adding different concentrations of the inducer arabinose at 0-20 mM under fully aerobic conditions
Singh, R.S.; Gara, R.K.; Bhardwaj, P.K.; Kaachra, A.; Malik, S.; Kumar, R.; Sharma, M.; Ahuja, P.S.; Kumar, S.
Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]