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Information on EC 2.5.1.75 - tRNA dimethylallyltransferase and Organism(s) Homo sapiens and UniProt Accession Q9H3H1

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EC Tree
IUBMB Comments
Formerly known as tRNA isopentenyltransferase, but it is now known that prenyl diphosphate, rather than isopentenyl diphosphate, is the substrate.
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This record set is specific for:
Homo sapiens
UNIPROT: Q9H3H1
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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dimethylallyl diphosphate
+
adenine37 in tRNA
=
diphosphate
+
N6-dimethylallyladenine37 in tRNA
+
adenine37 in tRNA
=
+
N6-dimethylallyladenine37 in tRNA
Synonyms
prenyltransferase, trit1, trna isopentenyltransferase, trna-ipt, dmatase, trna ipt, mhipt3, noipt2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA2-isopentenyl pyrophosphate:transfer ribonucleic acid DELTA2-isopentenyltransferase
-
-
-
-
DELTA2-isopentenyl pyrophosphate:tRNA-DELTA2-isopentenyl transferase
-
-
-
-
isopentenyltransferase, transfer ribonucleate
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-
-
-
transfer ribonucleate isopentenyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate: tRNA dimethylallyltransferase
Formerly known as tRNA isopentenyltransferase, but it is now known that prenyl diphosphate, rather than isopentenyl diphosphate, is the substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-78-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + adenosine37 in tRNATrpCCA
N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
sequence contains a C2H2 Zn-finger-like motif
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
normal and tumor tissue
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Trit1 encodes an amino-terminal mitochondrial targeting sequence that directs mitochondrial import and modification of mitochondrial-tRNAs
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
Trit1 exhibits substrate-specific restriction against Schizosaccharomyces pombe cytosolic tRNATrpCCA-A37-A38. Position 32 of tRNATrpCCA may be a conditional determinant for substrate-specific i6A37 modification by Trit1
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MOD5_HUMAN
467
0
52725
Swiss-Prot
Mitochondrion (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R17E
mutation in the basic amphipathic helix component of the predicted mitochondrial targeting sequence, mutant accumulates mostly in nuclei and is not found to localize to mitochondria. Mutation decreases modification of mitochondrial but not cytosolic tRNAs upon expression in both Saccharomyces cerevisiae and Schizosaccharomyces pombe
R21E
mutation in the basic amphipathic helix component of the predicted mitochondrial targeting sequence, mutant accumulates mostly in nuclei and is not found to localize to mitochondria. Mutation decreases modification of mitochondrial but not cytosolic tRNAs upon expression in both Saccharomyces cerevisiae and Schizosaccharomyces pombe
T32A
mutant is inactive on both the mitochondrial and cytosolic tRNA substrates
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana tabacum cultivar Wisconsin 38
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
identification of compound heterozygous missense mutations, i.e. c.244A>G p.(Met82Val) in exon 2 in the maternally derived allele, and c.1034A>G p.(Tyr345Cys) in exon 9 in the paternally derived allele, in Trit1 in a girl with a developmental delay, frequent episodes of seizures induced by febrile illness, and myoclonic epilepsy. In peripheral blood and urine, a marked decrease in both i6A and ms2i6A modifications is observed. The mitochondrial disorder is caused by defective tRNA isopentenylation arising from a loss-of-function mutation in Trit1
medicine
expression of full-length transcript is downregulated 6-14fold in lung adenocarcinomas compared to normal tissue. A549-cells transfected to express the functional enzyme form significantly smaller colonies with reduced scattering on the edges and have only limited ability to induce tumors in nude mice
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Golovko, A.; Hjalm, G.; Sitbon, F.; Nicander, B.
Cloning of a human tRNA isopentenyl transferase
Gene
258
85-93
2000
Homo sapiens (Q9H3H1)
Manually annotated by BRENDA team
Spinola, M.; Galvan, A.; Pignatiello, C.; Conti, B.; Pastorino, U.; Nicander, B.; Paroni, R.; Dragani, T.A.
Identification and functional characterization of the candidate tumor suppressor gene TRIT1 in human lung cancer
Oncogene
24
5502-5509
2005
Homo sapiens (Q3T7C6), Homo sapiens
Manually annotated by BRENDA team
Golovko, A.; Sitbon, F.; Tillberg, E.; Nicander, B.
Expression of a human tRNA isopentenyltransferase in tobacco reveals a developmental role for tRNA isopentenyladenosine
Funct. Plant Biol.
34
654-661
2007
Homo sapiens (Q9H3H1)
Manually annotated by BRENDA team
Lamichhane, T.N.; Blewett, N.H.; Maraia, R.J.
Plasticity and diversity of tRNA anticodon determinants of substrate recognition by eukaryotic A37 isopentenyltransferases
RNA
17
1846-1857
2011
Saccharomyces cerevisiae (P07884), Saccharomyces cerevisiae, Homo sapiens (Q9H3H1), Homo sapiens, Schizosaccharomyces pombe (Q9UT75), Schizosaccharomyces pombe
Manually annotated by BRENDA team
Takenouchi, T.; Wei, F.Y.; Suzuki, H.; Uehara, T.; Takahashi, T.; Okazaki, Y.; Kosaki, K.; Tomizawa, K.
Noninvasive diagnosis of TRIT1-related mitochondrial disorder by measuring i6 A37 and ms2 i6 A37 modifications in tRNAs from blood and urine samples
Am. J. Med. Genet. A
179
1609-1614
2019
Homo sapiens (Q9H3H1), Homo sapiens
Manually annotated by BRENDA team
Khalique, A.; Mattijssen, S.; Haddad, A.F.; Chaudhry, S.; Maraia, R.J.
Targeting mitochondrial and cytosolic substrates of TRIT1 isopentenyltransferase Specificity determinants and tRNA-i6A37 profiles
PLoS Genet.
16
e1008330
2020
Homo sapiens (Q9H3H1), Homo sapiens
Manually annotated by BRENDA team