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Information on EC 2.5.1.75 - tRNA dimethylallyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P07884

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EC Tree
IUBMB Comments
Formerly known as tRNA isopentenyltransferase, but it is now known that prenyl diphosphate, rather than isopentenyl diphosphate, is the substrate.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P07884
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
+
adenine37 in tRNA
=
+
N6-dimethylallyladenine37 in tRNA
Synonyms
prenyltransferase, trit1, trna isopentenyltransferase, trna-ipt, dmatase, trna ipt, mhipt3, noipt2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA2-isopentenyl pyrophosphate:transfer ribonucleic acid DELTA2-isopentenyltransferase
-
-
-
-
DELTA2-isopentenyl pyrophosphate:tRNA-DELTA2-isopentenyl transferase
-
-
-
-
isopentenyltransferase, transfer ribonucleate
-
-
-
-
transfer ribonucleate isopentenyltransferase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + adenine37 in tRNA = diphosphate + N6-dimethylallyladenine37 in tRNA
show the reaction diagram
ordered substrate binding
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate: tRNA dimethylallyltransferase
Formerly known as tRNA isopentenyltransferase, but it is now known that prenyl diphosphate, rather than isopentenyl diphosphate, is the substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-78-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + adenosine 37 in tRNACys
N6-(dimethylallyl)adenosine37 in tRNACys + diphosphate
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + adenosine 37 in tRNASer
N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA
N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + adenosine 37 in tRNATyr
N6-(dimethylallyl)adenosine37 in tRNATyr + diphosphate
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
show the reaction diagram
-
biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
can partially replace Mg2+ in activation
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetamide
-
-
p-mercuribenzoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
-
pH 7: about 95% of maximum activity, pH 9.5: about 45% of maximum activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DMATase-tRNACys complex in four distinct forms, which provide snapshots of the RNA modification reaction catalyzed by DMATase
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K127D
activity similar to wild-type
K181H
selective disproportionate decrease in activity toward several substrates
Y84S
activity similar to wild-type
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kline, L.K.; Fittler, F.; Hall, R.H.
N6-(delta-2-isopentenyl) adenosine. Biosynthesis in transfer ribonucleic acid in vitro
Biochemistry
8
4361-4371
1969
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Zhou, C.; Huang, R.H.
Crystallographic snapshots of eukaryotic dimethylallyltransferase acting on tRNA: insight into tRNA recognition and reaction mechanism
Proc. Natl. Acad. Sci. USA
105
16142-16147
2008
Saccharomyces cerevisiae (P07884), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Lamichhane, T.N.; Blewett, N.H.; Maraia, R.J.
Plasticity and diversity of tRNA anticodon determinants of substrate recognition by eukaryotic A37 isopentenyltransferases
RNA
17
1846-1857
2011
Saccharomyces cerevisiae (P07884), Saccharomyces cerevisiae, Homo sapiens (Q9H3H1), Homo sapiens, Schizosaccharomyces pombe (Q9UT75), Schizosaccharomyces pombe
Manually annotated by BRENDA team