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Information on EC 2.5.1.74 - 1,4-dihydroxy-2-naphthoate polyprenyltransferase and Organism(s) Escherichia coli and UniProt Accession P32166

for references in articles please use BRENDA:EC2.5.1.74
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IUBMB Comments
This enzyme catalyses a step in the synthesis of menaquinone, in which the prenyl chain synthesized by polyprenyl diphosphate synthase is transferred to 1,4-dihydroxy-2-naphthoate (DHNA). The bacterial enzyme is an inner membrane protein , with the C-terminus located in the periplasm . It is highly specific for DHNA but not for a specific length of the prenyl chain .
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Escherichia coli
UNIPROT: P32166
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
1,4-dihydroxy-2-naphthoate octaprenyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,4-dihydroxy-2-naphthoate octaprenyltransferase
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SYSTEMATIC NAME
IUBMB Comments
all-trans-polyprenyl diphosphate:1,4-dihydroxy-2-naphthoate polyprenyltransferase
This enzyme catalyses a step in the synthesis of menaquinone, in which the prenyl chain synthesized by polyprenyl diphosphate synthase is transferred to 1,4-dihydroxy-2-naphthoate (DHNA). The bacterial enzyme is an inner membrane protein [1], with the C-terminus located in the periplasm [3]. It is highly specific for DHNA but not for a specific length of the prenyl chain [2].
CAS REGISTRY NUMBER
COMMENTARY hide
60063-82-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
farnesyl diphosphate + 1,4-dihydroxy-2-naphthoate
? + phosphate + CO2
show the reaction diagram
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-
?
all-trans-nonaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H+
demethylmenaquinol-9 + diphosphate + CO2
show the reaction diagram
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all-trans-nonaprenyl diphosphate = solanesyl pyrophosphate
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-
?
solanesyl diphosphate + 1,4-dihydroxy-2-naphthoate
demethylmenaquinone + phosphate + CO2
show the reaction diagram
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-
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?
additional information
?
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no substrate: solanesyl monophosphate
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shineberg, B.; Young, I.G.
Biosynthesis of bacterial menaquinones: the membrane-associated 1,4-dihydroxy-2-naphthoate octaprenyltransferase of Escherichia coli
Biochemistry
15
2754-2758
1976
Escherichia coli
Manually annotated by BRENDA team
Suvarna, K.; Stevenson, D.; Meganathan, R.; Hudspeth, M.E.S.
Menaquinone (vitamin K2) biosynthesis: localization and characterization of the menA gene from Escherichia coli
J. Bacteriol.
180
2782-2787
1998
Escherichia coli (P32166)
Manually annotated by BRENDA team