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Information on EC 2.5.1.73 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase and Organism(s) Methanococcus maripaludis and UniProt Accession A4FWT8

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EC Tree
IUBMB Comments
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism . Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) and EC 2.5.1.73 .
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Methanococcus maripaludis
UNIPROT: A4FWT8
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The taxonomic range for the selected organisms is: Methanococcus maripaludis
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms
sepcyss, sep-trna:cys-trna synthase, sep-trna-cys-trna synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Sep-tRNA:Cys-tRNA synthase
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
O-phospho-L-seryl-tRNACys:hydrogen sulfide 2-aminopropanoate transferase
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) and EC 2.5.1.73 [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-phospho-L-seryl-tRNACys + sulfide
L-cysteinyl-tRNACys + phosphate
show the reaction diagram
O-phospho-L-seryl-tRNACys + sulfide
L-cysteinyl-tRNACys + phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O-phospho-L-seryl-tRNACys + sulfide
L-cysteinyl-tRNACys + phosphate
show the reaction diagram
the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism containing a dispensable cysteinyl-tRNACys synthase, EC 6.1.1.16, overview
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O-phospho-L-seryl-tRNACys + sulfide
L-cysteinyl-tRNACys + phosphate
show the reaction diagram
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the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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pyridoxal 5'-phosphate
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene pcsS
SwissProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sauerwald, A.; Zhu, W.; Major, T.A.; Roy, H.; Palioura, S.; Jahn, D.; Whitman, W.B.; Yates, J.R.; Ibba, M.; Soell, D.
RNA-dependent cysteine biosynthesis in archea
Science
307
1969-1972
2005
Methanocaldococcus jannaschii, Methanococcus maripaludis (A4FWT8), Methanococcus maripaludis
Manually annotated by BRENDA team
Yuan, J.; Sheppard, K.; Soell, D.
Amino acid modifications on tRNA
Acta Biochim. Biophys. Sin. (Shanghai)
40
539-553
2008
Archaeoglobus fulgidus, Archaeoglobus fulgidus (O30207), Methanococcus maripaludis, no activity in Methanobrevibacter smithii, no activity in Methanosphaera stadtmanae
Manually annotated by BRENDA team