Any feedback?
No. of entries
Information on EC 2.5.1.57 - N-acylneuraminate-9-phosphate synthase
for references in articles please use BRENDA:EC2.5.1.57
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.5 Transferring alkyl or aryl groups, other than methyl groups
2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
2.5.1.57 N-acylneuraminate-9-phosphate synthase
IUBMB Comments
Acts on N-glycoloyl and N-acetyl-derivatives.
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
- 2.5.1.57
- n-acetylmannosamine
-
sialylation
-
2-keto-3-deoxy-d-glycero-d-galacto-nononic
- mannac
- glycoconjugates
- kdn
-
bottleneck
- glcnac
- pep
-
cmp-sialic
- mannose
- n-glycans
-
neunacs
- frugiperda
-
spodoptera
-
cmp-n-acetylneuraminic
- udp-glcnac
-
n-acetylmannosamine-6-phosphate
- udp-n-acetylglucosamine
- synthesis
Reaction Schemes
showSynonyms
neu5ac-9-p synthase, dmsas, n-acetylneuraminic acid phosphate synthase, neunac-9-p synthase, sialic acid 9-phosphate synthase, n-acetylneuraminic acid 9-phosphate synthase, n-acetylneuraminate 9-phosphate synthase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
EC 4.1.3.20
-
-
formerly
-
N-acetylneuraminate-9-phosphate lyase
-
-
-
-
N-acetylneuraminate-9-phosphate sialic acid 9-phosphate synthase
-
-
-
-
N-acetylneuraminate-9-phosphate synthetase
-
-
-
-
N-acetylneuraminic acid phosphate synthase
N-acylneuraminate-9-phosphate pyruvate-lyase (pyruvate-phosphorylating)
-
-
-
-
neuac-9-phosphate synthase
NeuNAc-9-P synthase
SAS
sialic acid 9-phosphate synthase
-
-
-
-
sialic acid synthase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
phosphoenolpyruvate + N-acyl-D-mannosamine 6-phosphate + H2O = N-acylneuraminate 9-phosphate + phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
condensation
Phosphorylation
-
-
-
-
condensation
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
MetaCyc
CMP-N-acetylneuraminate biosynthesis I (eukaryotes)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
phosphoenolpyruvate:N-acyl-D-mannosamine-6-phosphate 1-(2-carboxy-2-oxoethyl)transferase
Acts on N-glycoloyl and N-acetyl-derivatives.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9031-58-7
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-mannose 6-phosphate + phosphoenolpyruvate + H2O
2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate
D-mannose 6-phosphate + phosphoenolpyruvate + H2O
2-keto-3-deoxy-D-glycero-D-galactononoic acid 9-phosphate + phosphate
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetylneuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetylneuraminic acid 9-phosphate + phosphate
Substrates: -
Products: -
Products: -
?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminate 9-phosphate + phosphate
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminic acid 9-phosphate + phosphate
N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-glycolyl-neuraminate 9-phosphate + phosphate
D-mannose 6-phosphate + phosphoenolpyruvate + H2O
2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
D-mannose 6-phosphate + phosphoenolpyruvate + H2O
2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
Substrates: sialic acid biosynthesis
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
Q9JJH0
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: sialic acid metabolism, biosynthesis of N-acetyl-D-neuraminic acid via its 9-phosphate
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: final steps of the biosynthetic pathway of N-acetylneuraminic acid
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: sialic acid metabolism, biosynthesis of N-acetyl-D-neuraminic acid via its 9-phosphate
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: final steps of the biosynthetic pathway of N-acetylneuraminic acid
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: N-acetylneuraminic acid biosynthesis
Products: -
Products: -
?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminate 9-phosphate + phosphate
Substrates: -
Products: -
Products: -
?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminic acid 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminic acid 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acylneuraminic acid 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-glycolyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-glycolyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-glycolyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
Substrates: sialic acid biosynthesis
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
Q9JJH0
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: sialic acid metabolism, biosynthesis of N-acetyl-D-neuraminic acid via its 9-phosphate
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: final steps of the biosynthetic pathway of N-acetylneuraminic acid
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: sialic acid metabolism, biosynthesis of N-acetyl-D-neuraminic acid via its 9-phosphate
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: final steps of the biosynthetic pathway of N-acetylneuraminic acid
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
N-acetyl-neuraminate 9-phosphate + phosphate
-
Substrates: N-acetylneuraminic acid biosynthesis
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Mg2+
Mn2+
Ni2+
additional information
Mn2+
activity is favored by the presence of Mn2+ ions at a concentration of 12.5 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Mg2+
12.5 mM Mg2+ decrease the ability for N-acetylneuraminate 9-phosphate synthesis by almost half, while the 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthetic activity drops by 20%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.22
in the presence of 12.5 mM MnCl2, 8.3 mM phosphoenolpyruvate and 12.5 mM D-mannose 6-phosphate
11.7
in the presence of 12.5 mM MnCl2, 8.3 mM phosphoenolpyruvate and 2.5 mM N-acyl-D-mannosamine 6-phosphate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
neuB gene product amino acid sequence accession. no.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line Links Show AA Sequence and Transmembrane Helices (124 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40000
41000
42360
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
dimer or trimer
-
the molecular weight is more than the size of a dimer, but smaller than a trimer