Information on EC 2.5.1.51 - beta-pyrazolylalanine synthase

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The expected taxonomic range for this enzyme is: Citrullus lanatus subsp. vulgaris

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EC NUMBER
COMMENTARY hide
2.5.1.51
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RECOMMENDED NAME
GeneOntology No.
beta-pyrazolylalanine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-acetyl-L-serine + pyrazole = 3-(pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminopropanoyl-group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-pyrazole-1-ylalanine biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
O3-acetyl-L-serine:pyrazole 1-(2-amino-2-carboxyethyl)transferase
The enzyme is highly specific for acetylserine and pyrazole. Not identical with EC 2.5.1.52 L-mimosine synthase.
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CAS REGISTRY NUMBER
COMMENTARY hide
37290-81-6
not distinguished from EC 4.2.1.50 in Chemical Abstracts
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-succinyl-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + succinate
show the reaction diagram
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at 16% the rate of O-acetyl-L-serine
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-
?
O-sulfo-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + SO42-
show the reaction diagram
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at 2.5% the rate of O-acetyl-L-serine
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-
?
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
show the reaction diagram
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at 11% the rate of pyrazole
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-
O3-acetyl-L-serine + N-hydroxyurea
O-ureidoserine + acetate
show the reaction diagram
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at 9% the rate of pyrazole
-
?
O3-acetyl-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O3-acetyl-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
-
involved in biosynthesis of heterocyclic beta-substituted alanines
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-
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
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50 mM, almost complete inhibition
KCN
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10 mM, almost complete inhibition
additional information
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no inhibition by pyridoxal phosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
O-acetylserine
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
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about half-maximal activity at pH 6.5 and 8.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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barely detectable in dry seeds
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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2 * 32000, SDS-PAGE
58000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 32000, SDS-PAGE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freeze-thawing or lyophilization inactivates
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sensitive to freezing even in the presence of 20% glycerol
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-2°C, stable for at least 1 month
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0°C, crude enzyme preparation, 25% loss of activity within 22 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.5.1.51)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)