enzyme activity gradually increases in the temperature range from 10 to 40°C. The enzyme activity is less than half of the highest activity when the temperature is not more than 30°C and higher than 60°C. The enzyme activity is completely absent at 70°C
mucopolysaccharidose, upon silencing significant increase of sulfated substrates, due to overexpression significant decline of all substrates and up-regulation of chondroitin sulfate-containing proteoglycan (syndecan-1 and decorin) expression, increase of soluble syndecan-1 protein
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the addition of 5% (w/v) sucrose and trehalose prior to lyophilization can retain nearly 100% enzymatic activity, compared to 19% for the lyophilized control. Polyol sucrose and amino acid glycine enhance the thermal stability of the enzyme, while polyol mannitol decreases the thermal stability