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Information on EC 2.5.1.46 - deoxyhypusine synthase and Organism(s) Bos taurus and UniProt Accession Q6EWQ6

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IUBMB Comments
The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity. This enzyme catalyses the first reaction of hypusine formation from one specific lysine residue of the eIF5A precursor. The reaction occurs in four steps: NAD+-dependent dehydrogenation of spermidine (1a), formation of an enzyme-imine intermediate by transfer of the 4-aminobutylidene group from dehydrospermidine to the active site lysine residue (Lys329 for the human enzyme; 1b), transfer of the same 4-aminobutylidene group from the enzyme intermediate to the e1F5A precursor (1c), reduction of the e1F5A-imine intermediate to form a deoxyhypusine residue (1d). Hence the overall reaction is transfer of a 4-aminobutyl group. For the plant enzyme, homospermidine can substitute for spermidine and putrescine can substitute for the lysine residue of the eIF5A precursor. Hypusine is formed from deoxyhypusine by the action of EC 1.14.99.29, deoxyhypusine monooxygenase.
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This record set is specific for:
Bos taurus
UNIPROT: Q6EWQ6
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota
Synonyms
deoxyhypusine synthase, dhs34, cpdhs, pf3d7_1412600, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
deoxyhypusine synthase (Caulobacter crescentus gene CC0359)
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deoxyhypusine synthase (Halobacterium strain NRC-1 gene dhs)
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deoxyhypusine synthase (human clone 30649 gene DHPS subunit reduced)
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deoxyhypusine synthase (Nicotiana tabacum gene DHS1)
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deoxyhypusine synthase (Senecio vernalis gene DHS1)
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DHS
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synthase, deoxyhypusine
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminobutyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
[eIF5A-precursor]-lysine:spermidine 4-aminobutyltransferase (propane-1,3-diamine-forming)
The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity. This enzyme catalyses the first reaction of hypusine formation from one specific lysine residue of the eIF5A precursor. The reaction occurs in four steps: NAD+-dependent dehydrogenation of spermidine (1a), formation of an enzyme-imine intermediate by transfer of the 4-aminobutylidene group from dehydrospermidine to the active site lysine residue (Lys329 for the human enzyme; 1b), transfer of the same 4-aminobutylidene group from the enzyme intermediate to the e1F5A precursor (1c), reduction of the e1F5A-imine intermediate to form a deoxyhypusine residue (1d). Hence the overall reaction is transfer of a 4-aminobutyl group. For the plant enzyme, homospermidine can substitute for spermidine and putrescine can substitute for the lysine residue of the eIF5A precursor. Hypusine is formed from deoxyhypusine by the action of EC 1.14.99.29, deoxyhypusine monooxygenase.
CAS REGISTRY NUMBER
COMMENTARY hide
127069-31-2
deoxyhypusine synthase
171041-87-5
human clone 30649 gene DHPS subunit reduced
253180-41-5
Nicotiana tabacum gene DHS1
259168-76-8
Senecio vernalis gene DHS1
302492-11-1
Halobacterium strain NRC-1 gene dhs
332963-67-4
Caulobacter crescentus gene CC0359
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
show the reaction diagram
deoxyhypusine synthase is the first of the two enzymes that catalyze the maturation of eukaryotic initiation factor 5A. Synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DHYS_BOVIN
369
0
41029
Swiss-Prot
other Location (Reliability: 3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
subcloning of bovine and human DHS gene, overexpression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Huang, J.K.; Tsai, S.; Huang, G.H.; Sershon, V.C.; Alley, A.M.; Wen, L.
Molecular cloning of bovine eIF5A and deoxyhypusine synthase cDNA
DNA Seq.
15
26-32
2004
Bos taurus (Q6EWQ6), Bos taurus
Manually annotated by BRENDA team
Huang, J.K.; Tsai, S.; Huang, G.H.; Gowda, P.G.; Walzer, A.M.; Wen, L.
Higher activity of recombinant bovine deoxyhypusine synthase vs. human deoxyhypusine synthase
Protein Expr. Purif.
35
32-38
2004
Bos taurus (Q6EWQ6), Bos taurus, Homo sapiens
Manually annotated by BRENDA team