Any feedback?
Information on EC 2.5.1.43 - nicotianamine synthase and Organism(s) Hordeum vulgare and UniProt Accession Q9ZQV7
for references in articles please use BRENDA:EC2.5.1.43Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.5.1.43 nicotianamine synthaseSpecify your search results
Word Map
- 2.5.1.43
-
abstinence
- infant
-
opioids
- women
-
nursing
-
nucleostide
-
naphthenic
- nafld
- steatohepatitis
- withdrawal
-
sands
- methadone
-
nonalcoholic
- steatosis
- morphine
-
services
- accumbens
- nurses
-
entecavir
- n-acetylserotonin
- buprenorphine
-
virological
-
policy
- tenofovir
-
hbeag
- oseltamivir
-
opiate
-
academy
- lamivudine
-
adefovir
- zanamivir
-
strictures
-
hbeag-positive
-
ukraine
-
nonpharmacologic
-
alberta
-
seroconversion
- neuraminidases
-
caring
-
telbivudine
-
mother-infant
-
dipivoxil
-
fe-deficient
- lilacs
-
staffing
-
nanoassemblies
-
medicaid
-
peg-ifn
-
disoproxil
-
hbeag-negative
- agriculture
The taxonomic range for the selected organisms is: Hordeum vulgare
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
nas, nicotianamine synthase, osnas2, osnas1, osnas3, chloronerva, atnas4, hvnas1, na synthase, atnas1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AtNAS1
-
-
-
-
AtNAS2
-
-
-
-
AtNAS3
-
-
-
-
chloronerva
-
-
-
-
HvNAS1
HvNAS2
-
-
-
-
HvNAS3
HvNAS4
HvNAS6
HvNAS7
-
-
-
-
NA synthase
NAS
NASHOR1
nicotianamine synthase
OsNAS1
-
-
-
-
OsNAS2
-
-
-
-
OsNAS3
-
-
-
-
S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase
-
-
-
-
synthase, nicotianamine
-
-
-
-
HvNAS1
-
-
-
-
HvNAS3
-
-
-
-
HvNAS4
-
-
-
-
HvNAS6
-
-
-
-
NA synthase
-
-
-
-
NAS
-
-
-
-
nicotianamine synthase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
alkenyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-Lmethionine 3-amino-3-carboxypropyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY
161515-44-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine
5'-S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
important role in production of nicotianamine under Fe-deficient conditions
-
-
?
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
important role in production of nicotianamine under Fe-deficient conditions
-
-
?
S-adenosyl-L-methionine
5'-S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
S-adenosyl-L-methionine
5'-S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
S-adenosyl-L-methionine
5'-S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
S-adenosyl-L-methionine
5'-S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
S-adenosyl-L-methionine
5'-S-methyl-5'-thioadenosine + nicotianamine
-
the product nictotinamine plays a critical role in metal detoxification
-
-
?
S-adenosyl-L-methionine
?
-
enzyme activity is induced at the 3rd day after withholding Fe supply and declines within one day following the supply of Fe3+-epihydroxymugineic acid
-
-
?
S-adenosyl-L-methionine
?
-
synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores
-
-
?
S-adenosyl-L-methionine
?
-
key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores
-
-
?
S-adenosyl-L-methionine
?
-
strongly induced by Fe-deficiency treatment
-
-
?
S-adenosyl-L-methionine
?
-
enzyme in the biosynthetic pathway of mugineic acids
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
important role in production of nicotianamine under Fe-deficient conditions
-
-
?
S-adenosyl-L-methionine
5'-S-methyl-5'-thioadenosine + nicotianamine
-
the product nictotinamine plays a critical role in metal detoxification
-
-
?
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
-
-
-
?
3 S-adenosyl-L-methionine
3 S-methyl-5'-thioadenosine + nicotianamine
important role in production of nicotianamine under Fe-deficient conditions
-
-
?
S-adenosyl-L-methionine
?
-
enzyme activity is induced at the 3rd day after withholding Fe supply and declines within one day following the supply of Fe3+-epihydroxymugineic acid
-
-
?
S-adenosyl-L-methionine
?
-
synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores
-
-
?
S-adenosyl-L-methionine
?
-
key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores
-
-
?
S-adenosyl-L-methionine
?
-
strongly induced by Fe-deficiency treatment
-
-
?
S-adenosyl-L-methionine
?
-
enzyme in the biosynthetic pathway of mugineic acids
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 10
-
pH 7.5: about 20% of maximal activity, pH 10.0: about 50% of maximal activity
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
enzyme activity is induced at the 3rd day after withholding Fe supply and decline within one day following the supply of Fe3+-epihydroxymugineic acid
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
important role in production of nicotianamine under Fe-deficient conditions
physiological function
intercellular transport of iron (Fe) in dicotyledonous plants
metabolism
physiological function
intercellular transport of iron (Fe) in dicotyledonous plants
metabolism
-
biosynthesis of nicotianamine, nicotianamine synthase catalyses the trimerization of S-adenosylmethionine and azetidine ring formation
metabolism
important role in production of nicotianamine under Fe-deficient conditions
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NAS2_HORVU
335
0
35841
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4
-
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 90% loss of activity
639734
5.5
-
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 80% loss of activity
639734
7
-
activity is almost completely recovered after overnight exposure in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl
639734
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
thiol proteases such as papain might digest nicotianamide synthase in crude extracts
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, stable for about 1 week
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in rice. Overexpression of the nicotianamine synthase gene enhances nicotianamine level, enhances the Fe and Zn concentrations and confers tolerance to Fe deficiency in calcareous soil
complementation of the Lycopersicon esculentum mutant chloronerva that is free of nicotinamine due to a defect in nicotinamide synthase
-
expressed in Nicotiana tabacum, expression is highly induced by Fe-deficiency in roots
-
expressed in Oryza sativa ssp. japonica cv. Taipei 309 under control of the 35S promoter from cauliflower mosaic virus after transformation by Agrobacterium tumefaciens
-
expression in rice. Overexpression of the nicotianamine synthase gene enhances nicotianamine level, enhances the Fe and Zn concentrations and confers tolerance to Fe deficiency in calcareous soil
expression in soybean, sweet potato, tobacco or rice. Overexpression of the nicotianamine synthase gene enhances nicotianamine level, enhances the Fe and Zn concentrations and confers tolerance to Fe deficiency in calcareous soil
expression of a barley HvNAS1 nicotinamine synthase gene promoter-gus fusion gene in transgenic tobacco is induced by Fe-deficiency in root
-
sweet potato plants (Ipomoea batatas) expressing the nicotianamine synthase 1 (HvNAS1) gene under the control of CaMV 35S promoter are produced by Agrobacterium-mediated transformation
transgenic Arabidopsis and tobacco plants constitutively overexpress the enzyme from Hordeum vulgare. Nicotianamine synthase overexpression results in increased biosynthesis of nicotianamine in transgenic plants, which conferrs enhanced tolerance of high levels of metals, particularly nickel, to plants. Promoter activities of four nicotianamine synthase genes in Arabidopsis are all increased in response to excess nickel, suggesting that nicotianamine plays an important role in the detoxification of nickel in plants. Transgenic tobacco plants with a high level of nicotianamine grew well in a nickel-enriched serpentine soil without developing any symptoms of nickel toxicity
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
overexpression of the nicotianamine synthase gene enhances nicotianamine levels in several crops, including crops in which endogenous nicotianamine is already high, such as soybean and sweet potato. Additionally, nicotianamine synthase overexpression enhances the Fe and Zn concentrations and confers tolerance to Fe deficiency in calcareous soil
agriculture
agriculture
-
the nicotianamine synthase gene may be a suitable candidate for making a transgenic plant tolerant to Fe-deficiency
agriculture
increasing the nicotianamine concentration of sweet potato by overexpression of the enzyme (HvNAS1) can significantly improve agricultural productivity and energy source. The transgenic sweet potato contains increased levels of Fe, Zn and nicotianamine, in the leaves
agriculture
overexpression of the nicotianamine synthase gene enhances nicotianamine levels in several crops, including crops in which endogenous nicotianamine is already high, such as soybean and sweet potato. Additionally, nicotianamine synthase overexpression enhances the Fe and Zn concentrations and confers tolerance to Fe deficiency in calcareous soil
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Higuchi, K.; Kanazawa, K.; Nishizawa, N.K.; Mori, S.
The role of nicotianamine synthase in response to Fe nutrition status in Gramineae
Plant Soil
178
171-177
1996
Hordeum vulgare, Oryza sativa, Zea mays
-
Higuchi, K.; Kanazawa, K.; Nishizawa, N.K.; Chino, M.; Mori, S.
Purification and characterization of nicotianamine synthase from Fe-deficient barley roots
Plant Soil
165
173-179
1994
Hordeum vulgare
-
Higuchi, K.; Suzuki, K.; Nakanishi, H.; Yamaguchi, H.; Nishizawa, N.K.; Mori, S.
Cloning of nicotianamine synthase genes, novel genes involved in the biosynthesis of phytosiderophores
Plant Physiol.
119
471-479
1999
Hordeum vulgare
Higuchi, K.; Watanabe, S.; Takahashi, M.; Kawasaki, S.; Nakanishi, H.; Nishizawa, N.K.; Mori, S.
Nicotianamine synthase gene expression differs in barley and rice under Fe-deficient conditions
Plant J.
25
159-167
2001
Hordeum vulgare, Oryza sativa
Higuchi, K.; Tani, M.; Nakanishi, H.; Yoshiwara, T.; Goto, F.; Nishizawa, N.K.; Mori, S.
The expression of a barley HvNAS1 nicotinamine synthase gene promoter-gus fusion gene in transgenic tobacco is induced by Fe-deficiency in root
Biosci. Biotechnol. Biochem.
65
1692-1696
2001
Hordeum vulgare
Schmiedeberg, L.; Krueger, C.; Stephan, U.W.; Baeumlein, H.; Hell, R.
Synthesis and proof-of-function of a [14C]-labelled form of the plant iron chelator nicotianamine using recombinant nicotianamine synthase from barley
Physiol. Plant.
118
430-438
2003
Hordeum vulgare
-
Kim, S.; Takahashi, M.; Higuchi, K.; Tsunoda, K.; Nakanishi, H.; Yoshimura, E.; Mori, S.; Nishizawa, N.K.
Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants
Plant Cell Physiol.
46
1809-1818
2005
Hordeum vulgare
Perovic, D.; Tiffin, P.; Douchkov, D.; Baeumlein, H.; Graner, A.
An integrated approach for the comparative analysis of a multigene family: the nicotianamine synthase genes of barley
Funct. Integr. Genomics
7
169-179
2007
Hordeum vulgare (Q9XFB6), Hordeum vulgare (Q9XFB7), Hordeum vulgare
Zhang, Z.; Zheng, Y.
Overexpression of nicotianamine synthase (NAS) gene results in enhanced drought tolerance in perennial ryegrass
Biotechnol. Biotechnol. Equip.
22
938-941
2008
Hordeum vulgare
-
Usuda, K.; Wada, Y.; Ishimaru, Y.; Kobayashi, T.; Takahashi, M.; Nakanishi, H.; Nagato, Y.; Mori, S.; Nishizawa, N.K.
Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect
Plant Biotechnol. J.
7
87-95
2009
Hordeum vulgare, Oryza sativa
Hakoyama, T.; Watanabe, H.; Tomita, J.; Yamamoto, A.; Sato, S.; Mori, Y.; Kouchi, H.; Suganuma, N.
Nicotianamine synthase specifically expressed in root nodules of Lotus japonicus
Planta
230
309-317
2009
Medicago truncatula (A2Q2X7), Oryza sativa Indica Group (A2XFU4), Oryza sativa Indica Group (A2XFU5), Oryza sativa Indica Group (A2YQ58), Lotus japonicus (B9A9Z1), Lotus japonicus (B9A9Z2), Lotus japonicus, Arabidopsis thaliana (O80483), Arabidopsis thaliana (Q6L5P3), Arabidopsis thaliana (Q9FF79), Arabidopsis thaliana (Q9FKT9), Zea mays (Q8LT22), Zea mays (Q8S9C5), Hordeum vulgare (Q9XFB6), Hordeum vulgare (Q9XFB7), Hordeum vulgare (Q9ZQV3), Hordeum vulgare (Q9ZQV6), Hordeum vulgare (Q9ZQV7), Hordeum vulgare (Q9ZQV8), Hordeum vulgare (Q9ZQV9), Solanum lycopersicum (Q9XGI7)
Ito, S.; Inoue, H.; Kobayashi, T.; Yoshiba, M.; Mori, S.; Nishizawa, N.; Higuchi, K.
Comparison of the functions of the barley nicotianamine synthase gene HvNAS1 and rice nicotianamine synthase gene OsNAS1 promoters in response to iron deficiency in transgenic tobacco
Soil Sci. Plant Nutr.
55
277-282
2009
Hordeum vulgare, Oryza sativa
-
Nozoye, T.
The nicotianamine synthase gene is a useful candidate for improving the nutritional qualities and Fe-deficiency tolerance of various crops
Front. Plant Sci.
9
340
2018
Oryza sativa (Q0D3F2), Oryza sativa (Q0DSH9), Oryza sativa (Q10MI9), Oryza sativa, Hordeum vulgare (Q9ZQV7), Hordeum vulgare (Q9ZQV8), Hordeum vulgare (Q9ZQV9)
Nozoye, T.; Otani, M.; Senoura, T.; Nakanishi, H.; Nishizawa, N.
Overexpression of barley nicotianamine synthase 1 confers tolerance in the sweet potato to iron deficiency in calcareous soil
Plant Soil
418
75-88
2017
Hordeum vulgare (Q9ZQV9)
-
EXTERNAL LINKS (specific for EC-Number 2.5.1.43)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
