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Information on EC 2.5.1.42 - geranylgeranylglycerol-phosphate geranylgeranyltransferase and Organism(s) Saccharolobus solfataricus and UniProt Accession Q9UWY6

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IUBMB Comments
This enzyme is an integral-membrane protein that carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in Archaea. Requires a divalent metal cation, such as Mg2+ or Mn2+, for activity . 4-Hydroxybenzoate, 1,4-dihydroxy 2-naphthoate, homogentisate and alpha-glycerophosphate cannot act as prenyl-acceptor substrates . The other enzymes involved in the biosynthesis of polar lipids in Archaea are EC 1.1.1.261 (sn-glycerol-1-phosphate dehydrogenase), EC 2.5.1.41 (phosphoglycerol geranylgeranyltransferase), which, together with this enzyme, alkylates the hydroxy groups of glycerol 1-phosphate to yield unsaturated archaetidic acid, which is acted upon by EC 2.7.7.67 [CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase] to form CDP-unsaturated archaeol. The final step in the pathway involves the addition of L-serine, with concomitant removal of CMP, leading to the production of unsaturated archaetidylserine . Belongs in the UbiA prenyltransferase family .
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Saccharolobus solfataricus
UNIPROT: Q9UWY6
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The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The enzyme appears in selected viruses and cellular organisms
Synonyms
(s)-2,3-di-o-geranylgeranylglyceryl phosphate synthase, dgggps, dgggp synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase
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geranylgeranyltransferase II
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geranylgeranyltransferase, geranylgeranyloxyglycerol phosphate
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl diphosphate:sn-3-O-(geranylgeranyl)glycerol 1-phosphate geranylgeranyltransferase
This enzyme is an integral-membrane protein that carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in Archaea. Requires a divalent metal cation, such as Mg2+ or Mn2+, for activity [2]. 4-Hydroxybenzoate, 1,4-dihydroxy 2-naphthoate, homogentisate and alpha-glycerophosphate cannot act as prenyl-acceptor substrates [2]. The other enzymes involved in the biosynthesis of polar lipids in Archaea are EC 1.1.1.261 (sn-glycerol-1-phosphate dehydrogenase), EC 2.5.1.41 (phosphoglycerol geranylgeranyltransferase), which, together with this enzyme, alkylates the hydroxy groups of glycerol 1-phosphate to yield unsaturated archaetidic acid, which is acted upon by EC 2.7.7.67 [CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase] to form CDP-unsaturated archaeol. The final step in the pathway involves the addition of L-serine, with concomitant removal of CMP, leading to the production of unsaturated archaetidylserine [3]. Belongs in the UbiA prenyltransferase family [2].
CAS REGISTRY NUMBER
COMMENTARY hide
124650-68-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate
diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
show the reaction diagram
additional information
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate
diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
show the reaction diagram
the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
divalent metal ion required, optimal activity with 10 mM Mg2+. 40% of maximal activity is obtrained with 10 mM Ca2+. 1 mM Ca2+ hardly activates
Mg2+
requirement of a divalent metal ion for activity. 1 mM Mg2+ gives 40% of the activity with 10 mM Mg2+
Mn2+
divalent metal ion required, optimal activity with 10 mM Mg2+. 10 mM Mn2+ leads to severely decreased activity
Zn2+
divalent metal ion required, optimal activity with 10 mM Mg2+. 10 mM Zn2+ leads to severely decreased activity. 1 mM Zn2+ hardly activates
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
integral membrane protein
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
x * 28000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 28000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli with a hexameric histidine tag
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hemmi, H.; Shibuya, K.; Takahashi, Y.; Nakayama, T.; Nishino, T.
(S)-2,3-Di-O-geranylgeranylglyceryl phosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked membrane lipids
J. Biol. Chem.
279
50197-50203
2004
Saccharolobus solfataricus (Q9UWY6), Saccharolobus solfataricus
Manually annotated by BRENDA team