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Information on EC 2.5.1.36 - trihydroxypterocarpan dimethylallyltransferase and Organism(s) Glycine max and UniProt Accession B9A1Q4
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2.5.1.36 trihydroxypterocarpan dimethylallyltransferaseIUBMB Comments
Part of the glyceollin biosynthesis system in soy bean.
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Word Map
- 2.5.1.36
- soybean
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phytoalexins
- prenyltransferase
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prenylation
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isoflavonoids
- glyceollins
- plastid
- isoflavone
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isoprenoid
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phytyltransferase
The taxonomic range for the selected organisms is: Glycine max
The expected taxonomic range for this enzyme is: Glycine max
The expected taxonomic range for this enzyme is: Glycine max
Reaction Schemes
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Synonyms
glycinol 4-dimethylallyltransferase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(6aS,11aS)-3,9,6a-trihydroxypterocarpan [(2)-glycinol] 4-dimethylallyltransferase
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dimethylallyl-diphosphate:(6aS,11aS)-3,6a,9-trihydroxypterocarpan dimethylallyltransferase
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dimethylallylpyrophosphate:3,6a,9-trihydroxypterocarpan dimethylallyltransferase
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dimethylallylpyrophosphate:trihydroxypterocarpan dimethylallyl transferase
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dimethylallytransferase, trihydroxypterocarpan
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glyceollin synthase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
alkenyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:(6aS,11aS)-3,6a,9-trihydroxypterocarpan dimethylallyltransferase
Part of the glyceollin biosynthesis system in soy bean.
CAS REGISTRY NUMBER
COMMENTARY
70851-94-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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-
-
?
dimethylallyl diphosphate + (-)-maackiain
diphosphate + ?
poor substrate
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-
?
dimethylallyl diphosphate + (6aS,11aS)-3,6a,9-trihydroxypterocarpan
diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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-
-
?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
dimethylallyl diphosphate + (-)-glycinol
diphosphate + 2-dimethylallylglycinol
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-
-
?
dimethylallyl diphosphate + (-)-glycinol
diphosphate + 4-dimethylallylglycinol
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-
-
?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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-
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?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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-
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?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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-
-
?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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introduction of the dimethylallyl substituent into trihydroxypterocarpan increases fungitoxicity against Cladosporium cucumerinum
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?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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involved in biosynthesis of glyceollins
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?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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enzyme is involved in synthesis of 2-dimethylallyl-trihydroxypterocarpan and 4-dimethylallyl-trihydroxypterocarpan, putative precursor of the glyceollins
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?
additional information
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glycinol 4-dimethylallyltransferase G4DT is specific for pterocarpan substrates
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?
additional information
?
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glycinol 4-dimethylallyltransferase G4DT is specific for pterocarpan substrates
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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introduction of the dimethylallyl substituent into trihydroxypterocarpan increases fungitoxicity against Cladosporium cucumerinum
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-
?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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involved in biosynthesis of glyceollins
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-
?
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
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enzyme is involved in synthesis of 2-dimethylallyl-trihydroxypterocarpan and 4-dimethylallyl-trihydroxypterocarpan, putative precursor of the glyceollins
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
Mg2+
requirement for divalent cations, with Mg2+ being more effective than Mn2+
Mn2+
requirement for divalent cations, with Mg2+ being more effective than Mn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
45
(6aS,11aS)-3,6a,9-trihydroxypterocarpan
using microsomes of cultured soybean cells
68
(6aS,11aS)-3,6a,9-trihydroxypterocarpan
using recombinant yeast microsomes
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
protein sequence is led by a plastid targeting signal sequence
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
the prenyltransferase is the key enzyme in the biosynthesis of prenylated flavonoids by catalyzing a rate-limiting step, i.e. the coupling process of two major metabolic pathways, the isoprenoid pathway and shikimate/polyketide pathway
evolution
(-)-glycinol 4-dimethylallyltransferase (G4DT) and (-)-glycinol 2-dimethylallyltransferase (G2DT) are paralogs derived from whole genome duplications of the soybean genome
metabolism
metabolism
(-)-glycinol 4-dimethylallyltransferase (G4DT) is involved in glyceollin I biosynthesis
metabolism
changes in G2DT gene expression are correlated with the induction of glyceollins II, III, IV and V in elicitor-treated soybean cells and leaves, suggesting its involvement in glyceollin biosynthesis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). G4DT_SOYBN
409
9
46012
Swiss-Prot
other Location (Reliability: 4)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cell culture
expression of the G4DT gene is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cultured soybean cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Leube, J.; Grisebach, H.
Further studies on induction of enzymes of phytoalexin synthesis in soybean and cultured soybean cells
Z. Naturforsch. C
38
730-735
1983
Glycine max
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Zaehringer, U.; Schaller, E.; Grisebach, H.
Induction of phytoalexin synthesis in soybean. Structure and reactions of naturally occuring and enzymatically prepared prenylated pterocarpans from elicitor-treated cotyledons and cell cultures of soybean
Z. Naturforsch. C
36
234-241
1981
Glycine max
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Zaehringer, U.; Ebel, J.; Mulheirn, L.J.; Lyne, R.L.; Grisebach, H.
Induction of phytoalexin synthesis in soybean. Dimethylallylpyrophosphate:trihydroxypterocarpan dimethylallyl transferase from elicitor-induced cotyledons
FEBS Lett.
101
90-92
1979
Glycine max
Akashi, T.; Sasaki, K.; Aoki, T.; Ayabe, S.; Yazaki, K.
Molecular cloning and characterization of a cDNA for pterocarpan 4-dimethylallyltransferase catalyzing the key prenylation step in the biosynthesis of glyceollin, a soybean phytoalexin
Plant Physiol.
149
683-693
2009
Glycine max (B9A1Q4), Glycine max
Sasaki, K.; Tsurumaru, Y.; Yamamoto, H.; Yazaki, K.
Molecular characterization of a membrane-bound prenyltransferase specific for isoflavone from Sophora flavescens
J. Biol. Chem.
286
24125-24134
2011
Glycine max (B9A1Q4), Glycine max
Yoneyama, K.; Akashi, T.; Aoki, T.
Molecular characterization of soybean pterocarpan 2-dimethylallyltransferase in glyceollin biosynthesis local gene and whole-genome duplications of prenyltransferase genes led to the structural diversity of soybean prenylated isoflavonoids
Plant Cell Physiol.
57
2497-2509
2016
Glycine max (A0A1L7NRS0), Glycine max (I1NJA2), Glycine max
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