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Information on EC 2.5.1.3 - thiamine phosphate synthase
for references in articles please use BRENDA:EC2.5.1.3
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EC Tree 2 Transferases
2.5 Transferring alkyl or aryl groups, other than methyl groups
2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
2.5.1.3 thiamine phosphate synthase
IUBMB Comments
The enzyme catalyses the penultimate reaction in thiamine de novo biosynthesis, condensing the pyrimidine and thiazole components. The enzyme is thought to accept the product of EC 2.8.1.10, thiazole synthase, as its substrate. However, it has been shown that in some bacteria, such as Bacillus subtilis, an additional enzyme, thiazole tautomerase (EC 5.3.99.10) converts that compound into its tautomer 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate, and that it is the latter that serves as the substrate for the synthase. In addition to this activity, the enzyme participates in a salvage pathway, acting on 4-methyl-5-(2-phosphooxyethyl)thiazole, which is produced from thiamine degradation products. In yeast this activity is found in a bifunctional enzyme (THI6) and in the plant Arabidopsis thaliana the activity is part of a trifunctional enzyme (TH1).
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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Synonyms
thin protein, thiamin phosphate synthase, tmp-ppase, tmppase, thiamine phosphate synthase, thiamine-phosphate pyrophosphorylase, thiamin-phosphate pyrophosphorylase, thiamine phosphate pyrophosphorylase, tmp pyrophosphorylase, thiamin phosphate pyrophosphorylase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-methyl-4-amino-5-hydroxymethylpyrimidinepyrophosphate:4-methyl-5-(2'-phosphoethyl)-thiazole 2-methyl-4-aminopyrimidine-5-methenyltransferase
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hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase
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bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
pyrophosphorylase, thiamin phosphate
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SSO2532
thiamin phosphate diphosphorylase
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bifunctional TMP diphosphorylase/HET kinase enzyme
thiamin phosphate pyrophosphorylase
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thiamin phosphate synthase
thiamin-phosphate pyrophosphorylase
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thiamine monophosphate pyrophosphorylase
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thiamine phosphate pyrophosphorylase
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thiamine phosphate synthase
thiamine-phosphate diphosphorylase
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thiamine-phosphate pyrophosphorylase
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thiamine-phosphate synthase
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thiaminephosphate pyrophosphorylase
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ThiE
ThiN protein
TM0723
TM1872
TMP pyrophosphorylase
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TMP-PPase
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TMPS
TPS
YjbQ
thiamin phosphate synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
4-amino-2-methyl-5-(diphosphomethyl)pyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2
(2)
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-
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4-amino-2-methyl-5-(diphosphomethyl)pyrimidine + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate = diphosphate + thiamine phosphate + CO2
4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)thiazole = diphosphate + thiamine phosphate
(3)
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4-amino-2-methyl-5-(diphosphomethyl)pyrimidine + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate = diphosphate + thiamine phosphate + CO2
dissociative SN1 mechanism
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4-amino-2-methyl-5-(diphosphomethyl)pyrimidine + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate = diphosphate + thiamine phosphate + CO2
(1)
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydroxymethylpyrimidine group transfer
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
MetaCyc
thiamine diphosphate biosynthesis I (E. coli), thiamine diphosphate biosynthesis II (Bacillus), thiamine diphosphate biosynthesis III (Staphylococcus), thiamine diphosphate biosynthesis IV (eukaryotes), thiamine diphosphate salvage II, thiamine diphosphate salvage IV (yeast), thiamine phosphate formation from pyrithiamine and oxythiamine (yeast)
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SYSTEMATIC NAME
IUBMB Comments
4-amino-2-methyl-5-diphosphomethylpyrimidine:2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate 4-amino-2-methylpyrimidine-5-methenyltransferase (decarboxylating)
The enzyme catalyses the penultimate reaction in thiamine de novo biosynthesis, condensing the pyrimidine and thiazole components. The enzyme is thought to accept the product of EC 2.8.1.10, thiazole synthase, as its substrate. However, it has been shown that in some bacteria, such as Bacillus subtilis, an additional enzyme, thiazole tautomerase (EC 5.3.99.10) converts that compound into its tautomer 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate, and that it is the latter that serves as the substrate for the synthase. In addition to this activity, the enzyme participates in a salvage pathway, acting on 4-methyl-5-(2-phosphooxyethyl)thiazole, which is produced from thiamine degradation products. In yeast this activity is found in a bifunctional enzyme (THI6) and in the plant Arabidopsis thaliana the activity is part of a trifunctional enzyme (TH1).
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CAS REGISTRY NUMBER
COMMENTARY
9030-30-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate
diphosphate + thiamine phosphate + CO2
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
diphosphate + thiamine phosphate + CO2
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
?
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Substrates: very low activity, almost 400-fold lower than the activity shown by the thiE-encoded thiamine-phosphate diphosphatase of Escherichia coli
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine phosphate + diphosphate
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-beta-hydroxyethylthiazole phosphate
thiamine phosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
4-amino-5-(hydroxymethyl)-2-(trifluoromethyl)pyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
?
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Substrates: poor substrate
Products: -
Products: -
?
4-amino-5-(hydroxymethyl)-2-methoxypyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
?
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Substrates: good substrate
Products: -
Products: -
?
4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate + 4-methyl-5-(2-hydroxyethyl)thiazole phosphate
thiamine monophosphate + diphosphate + phosphate
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
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Substrates: involved in biosynthesis of thiamine
Products: -
Products: -
?
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
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Substrates: expression is negatively regulated by thiamin
Products: -
Products: -
?
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
-
Substrates: involved in biosynthesis of thiamine
Products: -
Products: -
?
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
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Substrates: the bifunctional enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
r
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine phosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine phosphate + diphosphate
-
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
Substrates: thiamin biosynthesis
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
Substrates: -
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
Substrates: thiamin biosynthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: the condensation of 5-(2-hydroxyethyl)-4-methylthiazole phosphate and 4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate into thiamine monophosphate is catalyzed by the bifunctional TMP diphosphorylase/HET kinase encoded by the THI6 gene
Products: -
Products: -
?
additional information
?
-
Substrates: substrate 4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate cannot be replaced by 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate or 4-amino-5-hydroxymethyl-2-methylpyrimidine, nor substrate 4-methyl-5-(2-hydroxyethyl)thiazole phosphate by 4-methyl-5-(2-hydroxyethyl)thiazole
Products: -
Products: -
?
additional information
?
-
-
Substrates: the N-terminal domain of the bifunctional enzyme THI6 catalyzes the ligation of the thiamin thiazole and pyrimidine moieties to form thiamine phosphate, and the C-terminal domain catalyzes the phosphorylation of 4-methyl-5-hydroxyethylthiazole in a salvage pathway. Adenosine diphospho-5beta-ethyl-4-methylthiazole-2-carboxylic acid is not a substrate
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate
diphosphate + thiamine phosphate + CO2
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
diphosphate + thiamine phosphate + CO2
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
-
Substrates: involved in biosynthesis of thiamine
Products: -
Products: -
?
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
-
Substrates: expression is negatively regulated by thiamin
Products: -
Products: -
?
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
-
Substrates: involved in biosynthesis of thiamine
Products: -
Products: -
?
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
diphosphate + ?
-
Substrates: the bifunctional enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
Products: the enzyme catalyzes the tautomerization and decarboxylation of the intermediate (2R,5Z)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethylidene]-2,5-dihydro-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
diphosphate + thiamine phosphate + CO2
Substrates: -
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
Products: the enzyme catalyzes the decarboxylation of the intermediate 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxy-4-methyl-5-[2-(phosphonooxy)ethyl]-1,3-thiazol-3-ium
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
thiamine monophosphate + diphosphate
-
Substrates: contributes significantly to the balance of thiamine compounds during seed germination
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
Substrates: thiamin biosynthesis
Products: -
Products: -
?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
diphosphate + thiamine phosphate
Substrates: thiamin biosynthesis
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
Mg2+
an absolute requirement of Mg2+ for the activity is replaced to some extent by Co2+, Ca2+ or Zn2+. When Mg2+ is replaced by Mn2+, Ni2+ or Fe2+, however, the activity is much lower or not detected
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-methyl-4-amino-5-hydroxymethylpyrimidine
0.1 mM, 6.3% inhibition
2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate
0.1 mM, 18.2% inhibition
3-benzylsulfanyl-phenanthro[9,10-e][1,2,4]triazine
-
IC50 of 0.1 mg/ml
4-[[(2-hydroxy-5-nitrophenyl)methylidene]amino]-5-methyl-2-(propan-2-yl)phenol
-
IC50 of 0.034 mg/ml
7-[[4-chloro-6-(diethylamino)-s-triazin-2-yl]amino]-3-phenyl-coumarin
-
IC50 of more than 0.1 mg/ml
PCMB
-
0.01 mM, 98.5% inhibition, inhibition prevented by addition of 2-mercaptoethanol in 10fold molar excess
acetyl phosphate
-
0.5 mM, 50% inhibition, uncompetitive with respect to both substrates
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Tuberculosis
Identification of Inhibitors against Mycobacterium tuberculosis Thiamin Phosphate Synthase, an Important Target for the Development of Anti-TB Drugs.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0012
4-methyl-5-beta-hydroxyethylthiazole phosphate
-
wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C
0.0007
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate
-
wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C
0.001
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate
-
pH 9.2, 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.7
-
wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C
additional information
additional information
specific activity of 2.1 nmol thiamin phosphate formed/mg min
additional information
-
specific activity of 2.1 nmol thiamin phosphate formed/mg min
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 8.5
the optimum pH is pH 8.5, and the activity at pH 7.5 is about 60% of that at pH 8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
80 - 120
activity increases with an increase in temperature from 80°C to 120°C, and the highest activity is observed around 110°C. The activity at 100°C is about 75% of the highest activity
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
-
-
brenda
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
-
-
brenda
mutant resistant to 2-amino-4-methyl-5-beta-hydroxyethylthiazole, an antimetabolite of 4-methyl-5-beta-hydroxyethylthiazole, deficient in activity of both EC 2.5.1.3 and EC 2.7.1.50
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
2.5fold increase of activity over the resting cell level between the first and second day of germination
brenda
-
5fold increase of activity over the resting cell level between the first and second day of germination
brenda
-
progressive increase of activity towards the end of the 6-day germination period, 5fold
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction