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Information on EC 2.5.1.29 - geranylgeranyl diphosphate synthase and Organism(s) Sinapis alba and UniProt Accession Q43133

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IUBMB Comments
Some forms of this enzyme will also use geranyl diphosphate and dimethylallyl diphosphate as donors; it will not use larger prenyl diphosphates as efficient donors.
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This record set is specific for:
Sinapis alba
UNIPROT: Q43133
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The taxonomic range for the selected organisms is: Sinapis alba
The enzyme appears in selected viruses and cellular organisms
Synonyms
geranylgeranyl diphosphate synthase, ggpp synthase, ggdps, ggps1, ggpps1, cotb1, tgfpps, geranylgeranyl diphosphate synthase 1, geranylgeranyl pyrophosphate synthetase, ggppase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
geranylgeranyl diphosphate synthase
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GGPP synthase
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farnesyltransferase
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-
-
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geranylgeranyl diphosphate synthase
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-
-
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geranylgeranyl pyrophosphate synthase
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-
-
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geranylgeranyl pyrophosphate synthetase
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-
-
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geranylgeranyl-PP synthetase
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-
-
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synthetase, geranylgeranyl pyrophosphate
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-
-
-
additional information
the enzyme belongs to the FPP synthase family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate farnesyltranstransferase
Some forms of this enzyme will also use geranyl diphosphate and dimethylallyl diphosphate as donors; it will not use larger prenyl diphosphates as efficient donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-58-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans,trans-farnesyl diphosphate + isopentenyl diphosphate
diphosphate + geranylgeranyl diphosphate
show the reaction diagram
-
enzyme-product complex structure
-
?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
geranylgeranyl diphosphate + diphosphate
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
geranylgeranyl diphosphate + 3 diphosphate
show the reaction diagram
-
-
-
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
geranylgeranyl diphosphate + 2 diphosphate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trans,trans-farnesyl diphosphate + isopentenyl diphosphate
diphosphate + geranylgeranyl diphosphate
show the reaction diagram
-
-
-
?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
geranylgeranyl diphosphate + diphosphate
show the reaction diagram
-
-
-
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
geranylgeranyl diphosphate + 2 diphosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
the enzyme structure contains two DDxxD motifs and three Mg2+ ions
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
nitrogen-containing bisphosphonates
bind to the A-site, structure, overview
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Aminophenylethyl diphosphate
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
dimethylallyl diphosphate
-
-
0.033
isopentenyl diphosphate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
4300000 cpm/mg
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GGPPS_SINAL
366
0
39432
Swiss-Prot
Chloroplast (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
2 * 38000, SDS-PAGE
74000
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gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 38000, SDS-PAGE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type of selenomethionine-labeled enzyme, 20 mg/mL protein in 10 mM Tris-HCl, pH 7.9, 10 mM MgCl2, 100 mM NaCl, and 0.5 mM tris(2-carboxyethyl)phosphine, crystals of SeMet-labeled enzyme are obtained by vapor diffusion with hanging drops that contain a 1:1 mixture of protein solution and reservoir buffer D, the latter containing 100 mM sodium acetate, pH 4.4, 40% v/v propanediol, 10 mM MgCl2, and 0.5 mM TCEP, liquid nitrogen freezing without the addition of a cryo protectant, a second crystal form is obtained with native enzyme using the same procedure but with reservoir buffer E containing 100 mM N-cyclohexyl-3-aminopropane sulfonic acid, pH 10.0, 200 mM NaCl, 9-11% PEG 8000, 10 mM MgCl2, and 0.5 mM TCEP, mixing with reservoir buffer E containing 30% v/v glycerol and flash-freezing in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.8-2.0 A resolution, multiwavelength anomalous diffraction, soaking of native enzyme with FPP and Mg2+ fails, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
anion exchange chromatography, aminophenylethyl diphosphate affinity column
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of the His-tagged nezym ein Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Laferriere, A.; Beyer, P.
Purification of geranylgeranyl diphosphate synthase from Sinapis alba etioplasts
Biochim. Biophys. Acta
1077
167-172
1991
Sinapis alba
-
Manually annotated by BRENDA team
Kloer, D.P.; Welsch, R.; Beyer, P.; Schulz, G.E.
Structure and reaction geometry of geranylgeranyl diphosphate synthase from Sinapis alba
Biochemistry
45
15197-15204
2006
Sinapis alba (Q43133), Sinapis alba
Manually annotated by BRENDA team