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Information on EC 2.5.1.27 - adenylate dimethylallyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q94ID3

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IUBMB Comments
Involved in the biosynthesis of cytokinins in plants. Some isoforms from the plant Arabidopsis thaliana are specific for AMP while others also have the activity of EC 2.5.1.112, adenylate dimethylallyltransferase (ADP/ATP-dependent).
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q94ID3
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
isopentenyl transferase, isopentenyltransferase, sag12-ipt, atipt3, atipt5, adenosine phosphate-isopentenyltransferase, ipt5b, atipt7, adenylate isopentenyltransferase, atipt4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DMAPP:ATP/ADP isppentenyltransferase
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2-isopentenyl-diphosphate:AMP DELTA2-isopentenyltransferase
-
-
-
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adenosine phosphate-isopentenyltransferase
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ATP/ADP isopentenyltransferase
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cytokinin synthase
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-
-
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dimethylallyl-diphosphate:AMP DELTA2-isopentenyltransferase
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-
-
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dimethylallylpyrophosphate:5'-AMP transferase
-
-
-
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DMA transferase
-
-
-
-
isopentenyltransferase
-
-
-
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isopentenyltransferase, adenylate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:AMP dimethylallyltransferase
Involved in the biosynthesis of cytokinins in plants. Some isoforms from the plant Arabidopsis thaliana are specific for AMP while others also have the activity of EC 2.5.1.112, adenylate dimethylallyltransferase (ADP/ATP-dependent).
CAS REGISTRY NUMBER
COMMENTARY hide
72840-95-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + ADP
dimethylallyl-ADP + diphosphate
show the reaction diagram
the enzyme preferrs ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
show the reaction diagram
dimethylallyl diphosphate + ATP
dimethylallyl-ATP + diphosphate
show the reaction diagram
the enzyme preferrs ADP and ATP rather than AMP
-
-
?
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + AMP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + ADP
?
show the reaction diagram
-
the enzyme prefers ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + ADP
isopentenyl-ADP + diphosphate
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
show the reaction diagram
dimethylallyl diphosphate + ATP
?
show the reaction diagram
-
the enzyme prefers ADP and ATP rather than AMP
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
show the reaction diagram
biosynthesis of cytokinins
-
-
?
additional information
?
-
-
AtIPT3 is upregulated within 1 h after an application of nitrate zo mineral-strved Arabidopsis plants
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0065 - 0.05
dimethylallyl diphosphate
0.0083
dimethylallyl diphosphate
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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fruit abscission zone
Manually annotated by BRENDA team
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upper part of younger inflorescences
Manually annotated by BRENDA team
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AtIPT3, upregulated within 1 h after an application of nitrate zo mineral-strved Arabidopsis plants
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
farnesylated protein
Manually annotated by BRENDA team
-
farnesylated protein
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IPT1_ARATH
357
0
40765
Swiss-Prot
Chloroplast (Reliability: 1)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkylation
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farnesylation
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as fusions protein in Escherichia coi and Saccharomyces cerevisiae strains CTY716 and SYY705
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expression in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling. The method is based on EC 2.5.1.27 from Arabidopsis thaliana, EC 3.1.3.1 from calf intestine and EC 2.4.2.1 from Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Takei, K.; Sakakibara, H.; Sugiyama, T.
Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana
J. Biol. Chem.
276
26405-26410
2001
Arabidopsis thaliana (Q94ID3), Arabidopsis thaliana
Manually annotated by BRENDA team
Takei, K.; Sakakibara, H.; Sugiyama, T.
Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana
J. Biol. Chem.
276
2405-26410
2001
Arabidopsis thaliana
-
Manually annotated by BRENDA team
Takei, K.; Dekishima, Y.; Eguchi, T.; Yamaya, T.; Sakakibara, H.
A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling
J. Plant Res.
116
259-263
2003
Arabidopsis thaliana
Manually annotated by BRENDA team
Kakimoto, T.
Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases
Plant Cell Physiol.
42
677-685
2001
Arabidopsis thaliana (Q94ID3)
Manually annotated by BRENDA team
Miyawaki, K.; Matsumoto-Kitano, M.; Kakimoto, T.
Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate
Plant J.
37
128-138
2004
Arabidopsis thaliana
Manually annotated by BRENDA team
Sakakibara, H.; Kasahara, H.; Ueda, N.; Kojima, M.; Takei, K.; Hishiyama, S.; Asami, T.; Okada, K.; Kamiya, Y.; Yamaya, T.; Yamaguchi, S.
Agrobacterium tumefaciens increases cytokinin production in plastids by modifying the biosynthetic pathway in the host plant
Proc. Natl. Acad. Sci. USA
102
9972-9977
2005
Agrobacterium tumefaciens, Arabidopsis thaliana
Manually annotated by BRENDA team
Galichet, A.; Hoyerova, K.; Kaminek, M.; Gruissem, W.
Farnesylation directs AtIPT3 subcellular localization and modulates cytokinin biosynthesis in Arabidopsis
Plant Physiol.
146
1155-1164
2008
Arabidopsis thaliana
Manually annotated by BRENDA team