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Information on EC 2.5.1.21 - squalene synthase and Organism(s) Panax ginseng and UniProt Accession O48666

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EC Tree
IUBMB Comments
This microsomal enzyme catalyses the first committed step in the biosynthesis of sterols. The enzyme from yeast requires either Mg2+ or Mn2+ for activity. In the absence of NAD(P)H, presqualene diphosphate (PSPP) is accumulated. When NAD(P)H is present, presqualene diphosphate does not dissociate from the enzyme during the synthesis of squalene from farnesyl diphosphate (FPP) . High concentrations of FPP inhibit the production of squalene but not of PSPP .
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Panax ginseng
UNIPROT: O48666
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Word Map
The taxonomic range for the selected organisms is: Panax ginseng
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
farnesyltransferase, squalene synthase, sqs, fdft1, squalene synthetase, farnesyl-diphosphate farnesyltransferase, sgsqs, ssase, sqase, tksqs1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
farnesyl-diphosphate farnesyltransferase
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-
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farnesyldiphosphate:farnesyldiphosphate farnesyltransferase
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farnesyltransferase
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presqualene synthase
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presqualene-diphosphate synthase
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squalene synthase
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squalene synthetase
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synthase, squalene
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate:(2E,6E)-farnesyl-diphosphate farnesyltransferase
This microsomal enzyme catalyses the first committed step in the biosynthesis of sterols. The enzyme from yeast requires either Mg2+ or Mn2+ for activity. In the absence of NAD(P)H, presqualene diphosphate (PSPP) is accumulated. When NAD(P)H is present, presqualene diphosphate does not dissociate from the enzyme during the synthesis of squalene from farnesyl diphosphate (FPP) [8]. High concentrations of FPP inhibit the production of squalene but not of PSPP [8].
CAS REGISTRY NUMBER
COMMENTARY hide
9077-14-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
induction of enzyme by methyl jasmonate treatment, enhanced activity results in increase in phytosterols as well as ginsenoside contents
Manually annotated by BRENDA team
shoot apex
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SQS1_PANGI
415
2
47056
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
overexpression of enzyme in Eleutherococcus senticosus, results in enzyme activity up to 3fold higher than wild-type and increase in phytosterols beta-sitosterol and stigmasterol as well as in triterpene saponin levels
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Seo, J.W.; Jeong, J.H.; Shin, C.G.; Lo, S.C.; Han, S.S.; Yu, K.W.; Harada, E.; Han, J.Y.; Choi, Y.E.
Overexpression of squalene synthase in Eleutherococcus senticosus increases phytosterol and triterpene accumulation
Phytochemistry
66
869-877
2005
Panax ginseng (O48666), Panax ginseng
Manually annotated by BRENDA team
Lee, M.H.; Jeong, J.H.; Seo, J.W.; Shin, C.G.; Kim, Y.S.; In, J.G.; Yang, D.C.; Yi, J.S.; Choi, Y.E.
Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene
Plant Cell Physiol.
45
976-984
2004
Panax ginseng (O48666), Panax ginseng
Manually annotated by BRENDA team